Abstract
A rapid analytical method of human whole blood viscosity with low, medium, and high shear rates [WBV(L), WBV(M), and WBV(H), respectively] was developed using visible and near-infrared (Vis-NIR) spectroscopy combined with a moving-window partial least squares (MW-PLS) method. Two groups of peripheral blood samples were collected for modeling and validation. Separate analytical models were established for male and female groups to avoid interference in different gender groups and improve the homogeneity and prediction accuracy. Modeling was performed for multiple divisions of calibration and prediction sets to avoid over-fitting and achieve parameter stability. The joint analysis models for three indicators were selected through comprehensive evaluation of MW-PLS. The selected joint analysis models were 812–1278 nm for males and 670–1146 nm for females. The root-mean-square errors (SEP) and the correlation coefficients of prediction (RP) for all validation samples were 0.54 mPa•s and 0.91 for WBV(L), 0.25 mPa•s and 0.92 for WBV(M), and 0.22 mPa•s and 0.90 for WBV(H). Results indicated high prediction accuracy, with prediction values similar to the clinically measured values. Overall, the findings confirmed the feasibility of whole blood viscosity quantification based on Vis-NIR spectroscopy with MW-PLS. The proposed rapid and simple technique is a promising tool for surveillance, control, and treatment of cardio-cerebrovascular diseases in large populations.
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Abbreviations
- MW-PLS:
-
Moving-window partial least squares
- PLS:
-
Partial least squares
- RP :
-
Correlation coefficients for prediction
- RPD:
-
Ratio of standard error of performance to standard deviation
- SEP:
-
Root-mean-square errors for prediction
- Vis-NIR:
-
Visible and near-infrared
- WBV:
-
Whole blood viscosity
- WBV(H):
-
Whole blood viscosity at high shear rate
- WBV(L):
-
Whole blood viscosity at low shear rate
- WBV(M):
-
Whole blood viscosity at medium shear rate
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Acknowledgments
This work was supported by the Science and Technology Project of Guangdong Province of China (no. 2014A020212445, no. 2014A020213016).
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As the blood samples were collected and used in this study, the informed consent of all individual participants was obtained.
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Experiments were performed in compliance with the relevant laws and institutional guidelines and approved by local medical institution.
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Jiemei Chen, Zhiwei Yin, Yi Tang, Tao Pan declare that they have no conflict of interest.
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Chen, J., Yin, Z., Tang, Y. et al. Vis-NIR spectroscopy with moving-window PLS method applied to rapid analysis of whole blood viscosity. Anal Bioanal Chem 409, 2737–2745 (2017). https://doi.org/10.1007/s00216-017-0218-9
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DOI: https://doi.org/10.1007/s00216-017-0218-9