Abstract
A novel Faraday cage-type electrochemiluminescence (ECL) immunosensor devoted to the detection of Vibrio vulnificus (VV) was fabricated. The sensing strategy was presented by a unique Faraday cage-type immunocomplex based on immunomagnetic beads (IMBs) and multi-functionalized graphene oxide (GO) labeled with (2,2′-bipyridine)(5-aminophenanthroline)ruthenium (Ru-NH2). The multi-functionalized GO could sit on the electrode surface directly due to the large surface area, abundant functional groups, and good electronic transport property. It ensures that more Ru-NH2 is entirely caged and become “effective,” thus improving sensitivity significantly, which resembles extending the outer Helmholtz plane (OHP) of the electrode. Under optimal conditions, the developed immunosensor achieves a limit of detection as low as 1 CFU/mL. Additionally, the proposed immunosensor with high sensitivity and selectivity can be used for the detection of real samples. The novel Faraday cage-type method has shown potential application for the diagnosis of VV and opens up a new avenue in ECL immunoassay.
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Abbreviations
- Ab1 :
-
Anti-VV1
- Ab2 :
-
Anti-VV2
- APW:
-
Alkaline peptone water
- BSA:
-
Bovine serum albumin
- EC:
-
Enterobacter cloacae
- ECL:
-
Electrochemiluminescence
- ECLIA:
-
Electrochemiluminescence immunoassay
- EDC:
-
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride
- EIS:
-
Electrochemical impedance spectroscopy
- ELISA:
-
Enzyme-linked immunosorbent assay
- GLD:
-
Glutaraldehyde
- GO:
-
Graphene oxide
- IHP:
-
Interior Helmholtz plane
- IMBs:
-
Immunomagnetic beads
- LAMP:
-
Loop-mediated isothermal amplification
- MGCE:
-
Modified magnetic glass carbon electrode
- MPN:
-
Most probable number
- NHS:
-
N-Hydroxysuccinimide
- OHP:
-
Outer Helmholtz plane
- PCR:
-
Polymerase chain reaction
- Ru-NH2 :
-
(2,2′-Bipyridine)(5-aminophenanthroline)ruthenium
- SM:
-
Shewanella marisflavi
- TPrA:
-
Tripropylamine
- VH:
-
Vibrio harveyi
- VP:
-
Vibrio parahaemolyticus
- VV:
-
Vibrio vulnificus
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Financial supports from the National Natural Science Foundation of China (81273130, 41576098, 21571110), State Oceanic Administration of the People’s Republic of China (201105007), and Science and Technology Department of Zhejiang Province of China (2016C33176) are gratefully acknowledged. This work was also sponsored by K.C. Wong Magna Fund in Ningbo University.
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Published in the topical collection Analytical Electrochemiluminescence with guest editors Hua Cui, Francesco Paolucci, Neso Sojic, and Guobao Xu.
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Guo, Z., Sha, Y., Hu, Y. et al. Faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of Vibrio vulnificus based on multi-functionalized graphene oxide. Anal Bioanal Chem 408, 7203–7211 (2016). https://doi.org/10.1007/s00216-016-9851-y
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DOI: https://doi.org/10.1007/s00216-016-9851-y