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A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

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Abstract

Analytical techniques for detection of ochratoxin A (OTA) in food products and blood serum are of great significance. In this study, a fluorescent aptasensor was developed for sensitive and specific detection of OTA, based on a DNA pyramid nanostructure (DPN) and PicoGreen (PG) dye. The designed aptasensor inherits characteristics of DPN, such as high stability and capacity for PG loading. PG, as a fluorescent dye, could bind to double-stranded DNA (dsDNA). In the absence of OTA, the pyramid structure of DPN remains intact, leading to a very strong fluorescence emission. Because of higher affinity of aptamer for its target relative to its complementary strand, upon addition of target, the pyramid structure of DPN is disassembled, leading to a weak fluorescence emission. The presented aptasensor showed high specificity toward OTA with a limit of detection (LOD) as low as 0.135 nM. Besides, the designed sensing strategy was successfully utilized to recognize OTA in serum and grape juice with LODs of 0.184 and 0.149 nM, respectively.

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Acknowledgments

Financial support of this study was provided by Mashhad University of Medical Sciences.

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    Authors and Affiliations

    1. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Razavi Khorasan, Iran

      Morteza Alinezhad Nameghi & Faezeh Vahdati Hassani

    2. Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Razavi Khorasan, Iran

      Noor Mohammad Danesh & Mohammad Ramezani

    3. Research Institute of Sciences and New Technology, Mashhad, Razavi Khorasan, Iran

      Noor Mohammad Danesh

    4. Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Razavi Khorasan, Iran

      Khalil Abnous

    5. Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Razavi Khorasan, Iran

      Seyed Mohammad Taghdisi

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    1. Morteza Alinezhad Nameghi
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    2. Noor Mohammad Danesh
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    3. Mohammad Ramezani
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    Correspondence to Khalil Abnous or Seyed Mohammad Taghdisi.

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    Morteza Alinezhad Nameghi and Noor Mohammad Danesh contributed equally to this work.

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    Nameghi, M.A., Danesh, N.M., Ramezani, M. et al. A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A. Anal Bioanal Chem 408, 5811–5818 (2016). https://doi.org/10.1007/s00216-016-9693-7

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    • DOI: https://doi.org/10.1007/s00216-016-9693-7

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