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Quantitative evaluation of peptide-extraction methods by HPLC–triple-quad MS–MS

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Abstract

In this study, the efficiency of five peptide-extraction methods—acetonitrile (ACN) precipitation, ultrafiltration, C18 solid-phase extraction (SPE), dispersed SPE with mesoporous carbon CMK-3, and mesoporous silica MCM-41—was quantitatively investigated. With 28 tryptic peptides as target analytes, these methods were evaluated on the basis of recovery and reproducibility by using high-performance liquid chromatography–triple-quad tandem mass spectrometry in selected-reaction-monitoring mode. Because of the distinct extraction mechanisms of the methods, their preferences for extracting peptides of different properties were revealed to be quite different, usually depending on the pI values or hydrophobicity of peptides. When target peptides were spiked in bovine serum albumin (BSA) solution, the extraction efficiency of all the methods except ACN precipitation changed significantly. The binding of BSA with target peptides and nonspecific adsorption on adsorbents were believed to be the ways through which BSA affected the extraction behavior. When spiked in plasma, the performance of all five methods deteriorated substantially, with the number of peptides having recoveries exceeding 70 % being 15 for ACN precipitation, and none for the other methods. Finally, the methods were evaluated in terms of the number of identified peptides for extraction of endogenous plasma peptides. Only ultrafiltration and CMK-3 dispersed SPE performed differently from the quantitative results with target peptides, and the wider distribution of the properties of endogenous peptides was believed to be the main reason.

Distribution of the recoveries of target peptides after treatment by the five peptide-extraction methods

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Abbreviations

DSPE:

Dispersed solid-phase extraction

FA:

Formic acid

MW:

Molecular weight

pI:

Isoelectric point

QQQ:

Triple quadrupole

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Acknowledgments

The financial support by the National Nature Science Foundation of P. R. China (grant Nos. 21005080, 21275144, 21475131, 91317313, 21235005, 21321064) is gratefully acknowledged.

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Authors and Affiliations

  1. Key Laboratory of Separation Science for Analytical Chemistry, Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China

    Yan Du, Dapeng Wu, Qian Wu & Yafeng Guan

  2. Dalian Institute of Chemical Physics, University of the Chinese Academy of Sciences, Beijing, 100039, China

    Yan Du & Qian Wu

Authors
  1. Yan Du
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  2. Dapeng Wu
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  3. Qian Wu
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  4. Yafeng Guan
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Correspondence to Dapeng Wu or Yafeng Guan.

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Du, Y., Wu, D., Wu, Q. et al. Quantitative evaluation of peptide-extraction methods by HPLC–triple-quad MS–MS. Anal Bioanal Chem 407, 1595–1605 (2015). https://doi.org/10.1007/s00216-014-8389-0

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  • DOI: https://doi.org/10.1007/s00216-014-8389-0

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