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Detection of the food allergen celery via loop-mediated isothermal amplification technique

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Abstract

Since 2005, celery and celery products have to be labeled according to Directive 2003/89/EC due to their allergenic potential. In order to provide a DNA-based, rapid and simple detection method suitable for high-throughput analysis, a loop-mediated isothermal amplification (LAMP) assay for the detection of celery (Apium graveolens) was developed. The assay was tested for specificity for celery since closely related species also hold food relevance. The limit of detection (LOD) for spiked food samples was found to be as low as 7.8 mg of dry celery powder per kilogram. An evaluation of different amplification and detection platforms was performed to show reliable detection independent from the instrument used for amplification (thermal cycler or heating block) and detection mechanisms (real-time fluorescence detection, agarose gel electrophoresis or nucleic acid staining). The analysis of 10 commercial food samples representing diverse and complex food matrices, and a false-negative rate of 0 % for approximately 24 target copies or 0.08 ng celery DNA for three selected food matrices show that LAMP has the potential to be used as an alternative strategy for the detection of allergenic celery. The performance of the developed LAMP assay turned out to be equal or superior to the best available PCR assay for the detection of celery in food products.

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Acknowledgments

This work was financially supported by the Federal Country Lower Austria in cooperation with the European Regional Development Fund (ERDF).

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Authors and Affiliations

  1. Institute of Chemical Engineering, IFA-Tulln, Center for Analytical Chemistry, Vienna University of Technology, Konrad Lorenz Str. 20, 3430, Tulln, Austria

    Celine Zahradnik, Roland Martzy & Kurt Brunner

  2. Institute of Chemical Engineering, Gene Technology Group, Vienna University of Technology, Gumpendorfer Str. 1a, 1060, Vienna, Austria

    Robert L. Mach & Andreas H. Farnleitner

  3. Department IFA-Tulln, Center for Analytical Chemistry, University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria

    Rudolf Krska

Authors
  1. Celine Zahradnik
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  2. Roland Martzy
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  3. Robert L. Mach
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  4. Rudolf Krska
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  5. Andreas H. Farnleitner
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  6. Kurt Brunner
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Corresponding author

Correspondence to Kurt Brunner.

Additional information

Published in the topical collection Advances in Food Analysis with guest editors Michael W.F. Nielen, Jana Hajslova, and Rudolf Krska.

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Zahradnik, C., Martzy, R., Mach, R.L. et al. Detection of the food allergen celery via loop-mediated isothermal amplification technique. Anal Bioanal Chem 406, 6827–6833 (2014). https://doi.org/10.1007/s00216-014-7873-x

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  • DOI: https://doi.org/10.1007/s00216-014-7873-x

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