Abstract
The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.
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Acknowledgments
This work was supported by the National Basic Research Program of China (973 Program, no. 2011CB933600), the National Natural Science Foundation of China (No. 201372028) and the 863 program (SS2013AA031904).
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Huang, LL., Jin, YJ., Zhao, D. et al. A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system. Anal Bioanal Chem 406, 2687–2693 (2014). https://doi.org/10.1007/s00216-014-7651-9
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DOI: https://doi.org/10.1007/s00216-014-7651-9