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Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases

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Abstract

Transmembrane protein (TMP)-functionalized materials have resulted in powerful new methods in chemical analysis. Of particular interest is the development of high-throughput, TMP-functionalized stationary phases for affinity chromatography of complex mixtures of analytes. Several natural and synthetic phospholipids and lipid mimics have been used for TMP reconstitution, although the resulting membranes often lack the requisite chemical and temporal stability for long-term use, a problem that is exacerbated in flowing separation systems. Polymerizable lipids with markedly increased membrane stability and TMP functionality have been developed over the past two decades. More recently, these lipids have been incorporated into a range of analytical methods, including separation techniques, and are now poised to have a significant impact on TMP-based separations. Here, we describe current methods for preparing TMP-containing stationary phases and examine the potential utility of polymerizable lipids in TMP affinity chromatography.

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Abbreviations

bis-SorbPC:

1,2-bis[10-(2′,4′-hexadienoyloxy)decanoyl]-sn-glycero-3-phosphocholine

DiynePC:

1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine

GLUT1:

Type 1 glucose transporter

GPCR:

G-protein coupled receptor

IAM:

Immobilized artificial membrane

ILC:

Immobilized liposome chromatography

PSLB:

Planar supported lipid bilayer

PTPE:

1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine

SUV:

Small unilamellar vesicle

TMP:

Transmembrane protein

UV:

Ultraviolet

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Acknowledgements

This work was supported in part by the National Institutes of Health under grant number GM095763. The authors would like to express our gratitude to L. Kofi Bright Jr for assistance with the graphics.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA

    Elyssia S. Gallagher & Craig A. Aspinwall

  2. Applied Chemicals and Materials Division, National Institute of Standards and Technology, Boulder, CO, 80305, USA

    Elisabeth Mansfield

  3. BIO5 Institute, University of Arizona, Tucson, AZ, 85721, USA

    Craig A. Aspinwall

Authors
  1. Elyssia S. Gallagher
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  2. Elisabeth Mansfield
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  3. Craig A. Aspinwall
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Correspondence to Craig A. Aspinwall.

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Gallagher, E.S., Mansfield, E. & Aspinwall, C.A. Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases. Anal Bioanal Chem 406, 2223–2229 (2014). https://doi.org/10.1007/s00216-013-7545-2

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  • DOI: https://doi.org/10.1007/s00216-013-7545-2

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