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Enantioselective separation and simultaneous determination of fenarimol and nuarimol in fruits, vegetables, and soil by liquid chromatography–tandem mass spectrometry

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Abstract

A method for simultaneous enantioselective determination of fenarimol and nuarimol in apple, grape, cucumber, tomato, and soil was developed using liquid chromatography–tandem mass spectrometry. The enantioseparation results of the two fungicides through three different cellulose-based chiral columns are discussed. The influence of column temperature on the resolution of the enantiomers of the two fungicides was examined. Complete enantioseparation of the two fungicides’ enantiomers was obtained on a cellulose tris(4-methylbenzoate) column (Lux Cellulose-3) at 25 °C using methanol and 0.1 % formic acid solution (80:20, v/v) as mobile phase. The linearity, matrix effect, recovery, and precision were evaluated. Good linearity was obtained over the concentration range of 1–500 μg L−1 for each enantiomer in the standard solution and sample matrix calibration solution. There was no significant matrix effect in apple, grape, cucumber, or tomato samples, but signal suppression was typically observed with the soil extracts. The mean recoveries, repeatability, and reproducibility were 76.5–103 %, 2.1–9.0 %, and 4.2–11.8 %, respectively. The limit of quantification for enantiomers of the two fungicides in fruits, vegetables and soil was 5 μg kg−1. Moreover, the absolute configuration of the enantiomers of fenarimol and nuarimol was determined from a combination of experimentally determined and predicted electronic circular dichroism spectra.

Predicted ECD spectra of nuarimol enantiomers (a) and fenarimol enantiomers (b). Experimentally measured ECD spectra of nuarimol enantiomers (c) and fenarimol enantiomers (d) in acetonitrile (20 mg L−1)

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Acknowledgements

The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (no. 21107094), the National High Technology R&D Program of China (The 863 Program, no. 2011AA100806), and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences; State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control; MOA Key Laboratory for Pesticide Residue Detection, Hangzhou, 310021, China

    Hu Zhang, Xinquan Wang, Mingrong Qian, Xiangyun Wang, Hao Xu, Peipei Qi & Qiang Wang

  2. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China

    Shulin Zhuang

  3. Key Laboratory of Organosilicon Chemistry and Material Technology, Hangzhou Normal University, Hangzhou, 310012, China

    Kezhi Jiang

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  1. Hu Zhang
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  2. Xinquan Wang
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  3. Shulin Zhuang
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  5. Kezhi Jiang
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  9. Qiang Wang
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Correspondence to Qiang Wang.

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Zhang, H., Wang, X., Zhuang, S. et al. Enantioselective separation and simultaneous determination of fenarimol and nuarimol in fruits, vegetables, and soil by liquid chromatography–tandem mass spectrometry. Anal Bioanal Chem 404, 1983–1991 (2012). https://doi.org/10.1007/s00216-012-6325-8

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  • DOI: https://doi.org/10.1007/s00216-012-6325-8

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