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Fourier transform infrared imaging analysis in discrimination studies of St. John's wort (Hypericum perforatum)

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Abstract

In the present study, Fourier transform infrared (FTIR) imaging and data analysis methods were combined to study morphological and molecular patterns of St. John's wort (Hypericum perforatum) in detail. For interpretation, FTIR imaging results were correlated with histological information gained from light microscopy (LM). Additionally, we tested several evaluation processes and optimized the methodology for use of complex FTIR microscopic images to monitor molecular patterns. It is demonstrated that the combination of the used spectroscopic method with LM enables a more distinct picture, concerning morphology and distribution of active ingredients, to be gained. We were able to obtain high-quality FTIR microscopic imaging results and to distinguish different tissue types with their chemical ingredients.

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Abbreviations

API:

Active plant ingredients

ATR:

Attenuated total reflection

FFPE:

Formalin-fixed paraffin-embedded

FPA:

Focal plane array

FTIR:

Fourier transform infrared

HE:

Hematoxylin and eosin

HCA:

Hierarchical cluster analysis

HPLC:

High-performance liquid chromatography

IR:

Infrared

KM:

k-means

LM:

Light microscopy

MCT:

Mercury cadmium telluride

MIA:

Multivariate image analysis

MIR:

Mid-infrared

NIR:

Near-infrared

NIRS:

Near-infrared spectroscopy

PCA:

Principal component analysis

ROI:

Region of interest

TLC:

Thin-layer chromatography

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Acknowledgments

The authors would like to thank Prof. Heinz Siesler from the University of Duisburg-Essen, Germany for his aid in data analyses. We also gratefully acknowledge Mag. C. Seifarth from the Department of Urology, Medical University of Innsbruck for sectioning and for the assistance with sample preparation. The authors are grateful for the support of the Eurasia-Pacific Uninet (Salzburg, Austria), the Ministry for Science and Research, and the Ministry for Health, Family and Youth (Vienna, Austria) (Novel analytical tools for quality control in traditional Chinese medicine, project no. 80855).

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Authors and Affiliations

  1. Institute of Analytical Chemistry and Radiochemistry, CCB-Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80/82, 6020, Innsbruck, Austria

    V. A. Huck-Pezzei, J. D. Pallua, C. Pezzei, L. K. Bittner, S. A. Schönbichler, G. K. Bonn & C. W. Huck

  2. Bionorica SE, Kerschensteinerstrasse 11-18, 92318, Neumarkt, Germany

    G. Abel & M. Popp

Authors
  1. V. A. Huck-Pezzei
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  2. J. D. Pallua
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  3. C. Pezzei
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  4. L. K. Bittner
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  5. S. A. Schönbichler
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  6. G. Abel
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  7. M. Popp
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  8. G. K. Bonn
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  9. C. W. Huck
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Corresponding author

Correspondence to C. W. Huck.

Additional information

V. A. Huck-Pezzei, J. D. Pallua, and C. Pezzei contributed equally to this work.

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Huck-Pezzei, V.A., Pallua, J.D., Pezzei, C. et al. Fourier transform infrared imaging analysis in discrimination studies of St. John's wort (Hypericum perforatum). Anal Bioanal Chem 404, 1771–1778 (2012). https://doi.org/10.1007/s00216-012-6296-9

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  • DOI: https://doi.org/10.1007/s00216-012-6296-9

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