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Multi-walled carbon nanotubes as solid-phase extraction adsorbents for the speciation of cobalamins in seafoods by liquid chromatography

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Abstract

Multi-walled carbon nanotubes (MWCNTs) were evaluated as potential adsorbents for miniaturized solid-phase extraction coupled to liquid chromatography. The adsorption capacity of this sorbent was applied to assess the speciation of four cobalamins representing the various forms of vitamin B12. The preconcentration on the MWCNTs was based on the retention of analytes by introducing the sample online into the mini-column system. Dimethyl sulfoxide was used to elute the retained vitamins for liquid chromatographic analysis. The experimental conditions of the continuous flow device, which affect the enrichment procedure, such as the type and amount of nanotubes, the volume, pH and flow rate of the sample solution, and the eluent and its volume, were optimized. For detection purposes, a diode array device was used and good resolution was obtained with a mobile-phase acetonitrile–phosphate buffer and gradient elution. Specificity was demonstrated by the retention characteristics and UV spectra and by comparing the peak purity index with commercial standards. Linearity, precision, recovery, and sensitivity were satisfactory. Detection limits ranged from 0.35 to 30 ng mL−1. The method was successfully applied to the determination of cobalamins in seafoods, which were extracted from the sample with a buffer solution using an ultrasonic probe. The reliability of the procedure was checked by analyzing a certified reference material.

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References

  1. Gogotsi Y (2006) Nanomaterials handbook. CRC, Boca Raton

    Book  Google Scholar 

  2. Simón de Dios A, Díaz-García ME (2010) Anal Chim Acta 666:1–22

    Article  Google Scholar 

  3. Valcárcel M, Simonet BM, Cárdenas S, Suárez B (2005) Anal Bioanal Chem 382:1783–1790

    Article  Google Scholar 

  4. Valcárcel M, Cárdenas S, Simonet BM (2007) Anal Chem 79:4788–4797

    Article  Google Scholar 

  5. Valcárcel M, Cárdenas S, Simonet BM, Moliner-Martínez Y, Lucena R (2008) Trends Anal Chem 27:34–43

    Article  Google Scholar 

  6. Jiménez-Soto JM, Moliner-Martínez Y, Cárdenas S, Valcárcel M (2010) Electrophoresis 31:1681–1688

    Article  Google Scholar 

  7. Lucena R, Simonet BM, Cárdenas S, Valcárcel M (2011) J Chromatogr A 1218:620–637

    Article  CAS  Google Scholar 

  8. Cserháti T (2009) Biomed Chromatogr 23:111–118

    Article  Google Scholar 

  9. Kumar SS, Chouhan RS, Thakur MS (2010) Anal Biochem 398:139–149

    Article  CAS  Google Scholar 

  10. Yanes EG, Miller-Ihli J (2004) Spectrochim Acta Part B 59:891–899

    Article  Google Scholar 

  11. Nolan CV, Fowler SW, Teyssie JL (1992) Mar Ecol Prog Ser 88:105–116

    Article  CAS  Google Scholar 

  12. Jacobsen DW, Green R, Quadros EV, Montejano YD (1982) Anal Biochem 120:394–403

    Article  CAS  Google Scholar 

  13. Binder M, Kolhouse JF, Van Horne KC, Allen RH (1982) Anal Biochem 125:253–258

    Article  CAS  Google Scholar 

  14. Stupperich E, Steiner I, Rühlemann M (1986) Anal Biochem 155:365–370

    Article  CAS  Google Scholar 

  15. Djalali M, Gueant JL, Lambert D, El Kholty S, Saunier M, Nicolas JP (1990) J Chromatogr Biomed Appl 529:81–91

    Google Scholar 

  16. Ford SH, Gallery J, Nichols A, Shambee M (1991) J Chromatogr 537:235–247

    Article  CAS  Google Scholar 

  17. Lambert D, Adjalla C, Felden F, Benhayoun S, Nicolas JP, Guéant JL (1992) J Chromatogr 608:311–315

    Article  CAS  Google Scholar 

  18. Astier A, Baud FJ (1995) J Chromatogr B Biomed Appl 667:129–135

    Article  CAS  Google Scholar 

  19. Viñas P, López-Erroz C, Balsalobre N, Hernández-Córdoba M (2003) Chromatographia 58:5–10

    Google Scholar 

  20. Pakin C, Bergaentzlé M, Aoudé-Werner D, Hasselmann C (2005) J Chromatogr A 1081:182–189

    Article  CAS  Google Scholar 

  21. Viñas P, Campillo N, López-García I, Hernández-Córdoba M (1996) Anal Chim Acta 318:319–325

    Article  Google Scholar 

  22. Viñas P, Campillo N, López-García I, Hernández-Córdoba M (1996) Chromatographia 42:566–570

    Article  Google Scholar 

  23. Chassaigne H, Lobinski R (1998) Anal Chim Acta 359:227–235

    Article  CAS  Google Scholar 

  24. Chassaigne H, Szpunar J (1998) Analusis 26:M48–M51

    Article  CAS  Google Scholar 

  25. Lobinski R, Pereiro IR, Chassaigne H, Wasik A, Szpunar J (1998) J Anal Atom Spectrom 13:859–867

    Article  CAS  Google Scholar 

  26. Makarov A, Szpunar J (1999) J Anal At Spectrom 14:1323–1327

    Article  CAS  Google Scholar 

  27. Acon BW, McLean JA, Montaser A (2001) J Anal Atom Spectrom 16:852–857

    Article  CAS  Google Scholar 

  28. Yanes EG, Miller-Ihli NJ (2004) Spectrochim Acta Part B: Atom Spectrosc 59B:883–890

    Article  CAS  Google Scholar 

  29. Chassaigne H, Vacchina V, Lobinski R (2000) TrAC Trends Anal Chem 19:300–313

    Article  CAS  Google Scholar 

  30. Fred C, Haglund J, Alsberg T, Rydberg P, Minten J, Tornqvist M (2004) J Sep Sci 27:607–612

    Article  CAS  Google Scholar 

  31. Anes JM, Beck RA, Brink JJ, Goldberg RJ (1994) J Chromatogr B Biomed Appl 660:180–185

    Article  CAS  Google Scholar 

  32. Kelly RJ, Gruner TM, Sykes AR (2005) Biomed Chromatogr 19:329–333

    Article  CAS  Google Scholar 

  33. Kelly RJ, Gruner TM, Furlong JM, Sykes AR (2006) Biomed Chromatogr 20:806–814

    Article  CAS  Google Scholar 

  34. Hannibal L, Axhemi A, Glushchenko AV, Moreira ES, Brasch NE, Jacobsen DW (2008) Clin Chem Lab Med 46:1739–1746

    Article  CAS  Google Scholar 

  35. Suárez B, Santos B, Simonet BM, Cárdenas S, Valcárcel M (2007) J Chromatogr A 1175:127–132

    Article  Google Scholar 

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Acknowledgments

The authors are grateful to the Spanish MICINN (Project CTQ2009-08267/BQU) and to Fundación Séneca (Comunidad Autónoma de la Región de Murcia, Project 15217/PI/10) for financial support. M. Bravo Bravo also acknowledges a fellowship from Fundación Séneca.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071, Murcia, Spain

    Pilar Viñas, Ignacio López-García, María Bravo Bravo & Manuel Hernández-Córdoba

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  1. Pilar Viñas
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  2. Ignacio López-García
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  3. María Bravo Bravo
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  4. Manuel Hernández-Córdoba
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Correspondence to Manuel Hernández-Córdoba.

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Viñas, P., López-García, I., Bravo, M.B. et al. Multi-walled carbon nanotubes as solid-phase extraction adsorbents for the speciation of cobalamins in seafoods by liquid chromatography. Anal Bioanal Chem 401, 1393–1399 (2011). https://doi.org/10.1007/s00216-011-5158-1

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  • DOI: https://doi.org/10.1007/s00216-011-5158-1

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