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Identification of vitamin B12 analogues by liquid chromatography with electrothermal atomic absorption detection

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Summary

The coupling of liquid chromatography and electrothermal atomic absorption spectrometry (LC-ETAAS) lowers the detection limit for identification of vitamin B12 analogues. Cobalamins and aqueous cobalt (II) were separated by reversed-phase liquid chromatography using a linear gradient: 26∶74 (v/v) methanol:0.05 M phosphate buffer (pH 4.2) to 50∶50 mixture over 8 min. The vitamins were quantitatively determined in the column effluent by measuring total cobalt by ETAAS. The analysis of meat and liver extracts by LC-ETAAS showed that the matrix did not interfere with the determination of cobalt. Hence, recoveries of cobalt in spiked meat and liver samples were satisfactory.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071, Murcia, Spain

    P. Viñas, N. Campillo, I. López-García & M. Hernández-Córdoba

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  1. P. Viñas
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  2. N. Campillo
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  3. I. López-García
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  4. M. Hernández-Córdoba
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Viñas, P., Campillo, N., López-García, I. et al. Identification of vitamin B12 analogues by liquid chromatography with electrothermal atomic absorption detection. Chromatographia 42, 566–570 (1996). https://doi.org/10.1007/BF02290293

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  • DOI: https://doi.org/10.1007/BF02290293

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