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Evaluation of microwave-assisted enzymatic digestion and tandem mass spectrometry for the identification of protein residues from an inorganic solid matrix: implications in archaeological research

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Abstract

A method based on microwave-assisted enzymatic digestion and liquid chromatography–tandem mass spectrometry analysis is presented for the identification of proteins incorporated within solid matrices using protein standards bound to experimental cooking pottery as a validation model. The implementation of microwave irradiation allowed for a significant decrease in overall analysis time in addition to select enhancement of peptide recovery as determined by label-free relative quantitation. We envision that the reported methodology will provide new avenues for scientific discovery in areas such as archaeology and forensics. Results of this series of experiments are part of an ongoing project directed at developing a comprehensive methodology for extracting proteinaceous residues from archaeological pottery.

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Abbreviations

TOC:

Total organic carbon

SC:

Spectral counts

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Acknowledgments

This work was supported by the University of North Texas Health Science Center-University of North Texas Joint Institutional Seed Research Program Grant number G67718, the University of North Texas Research Initiation Grant Program Grant number G34478, and the National Science Foundation, Division of Behavioral and Cognitive Sciences, Archaeometry Technical Development Program Grant number 0822196. We thank Crow Canyon Archaeological Center for access to archaeological pottery samples and thoughtful support of this project. We thank ICBR Proteomics at the University of Florida for the use of their CEM Discover microwave system. We thank Art Goven and Kent Chapman for making this research possible.

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Authors and Affiliations

  1. Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, 33620, USA

    Stanley M. Stevens Jr & Kent W. Seeley

  2. Institute of Applied Sciences, Center for Environmental Archaeology, Department of Geography, University of North Texas, Denton, TX, 76203-5017, USA

    Steve Wolverton & Andrew Barker

  3. Institute of Applied Sciences, Department of Biological Sciences, University of North Texas, Denton, TX, 76203-5017, USA

    Barney Venables & Prem Adhikari

Authors
  1. Stanley M. Stevens Jr
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  2. Steve Wolverton
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  3. Barney Venables
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  4. Andrew Barker
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  5. Kent W. Seeley
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  6. Prem Adhikari
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Corresponding author

Correspondence to Stanley M. Stevens Jr.

Additional information

Stanley M. Stevens Jr and Steve Wolverton contributed equally to this work.

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Stevens, S.M., Wolverton, S., Venables, B. et al. Evaluation of microwave-assisted enzymatic digestion and tandem mass spectrometry for the identification of protein residues from an inorganic solid matrix: implications in archaeological research. Anal Bioanal Chem 396, 1491–1499 (2010). https://doi.org/10.1007/s00216-009-3341-4

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  • DOI: https://doi.org/10.1007/s00216-009-3341-4

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