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Chromatographic evaluation of polymers imprinted with analogs of chloramphenicol and application to selective solid-phase extraction

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Abstract

To obtain a highly selective material for the antibiotic chloramphenicol, which has several harmful side effects in humans, different molecularly imprinted polymers (MIPs) were prepared. In order to avoid a major traditional drawback associated with MIPs of residual template bleeding, molecules that are structurally related to chloramphenicol were used as templates for polymer synthesis. Chromatographic evaluation indicated that the employed template imparted a significant influence on the recognition properties of the corresponding polymer. A strong retention of chloramphenicol under nonpolar elution conditions (k = 68.03, IF = 17.72) and under aqueous elution conditions (k = 92.44, IF = 1.35) was achieved. After chromatographic evaluation, the MIP was utilized as the recognition sorbent in a solid-phase extraction to determine chloramphenicol using either an organic or aqueous washing solvent. Recoveries of nearly 100% from the chloramphenicol standard solution and nearly 90% from honey samples spiked with chloramphenicol were attained. Furthermore, the applicability of the MIP for sample cleanup was demonstrated.

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References

  1. Yunis AA (1988) Ann Rev 28:83–100

    CAS  Google Scholar 

  2. Holt D, Harvey D, Hurley R (1993) Adverse Drug React Toxicol Rev 12:83–95

    CAS  Google Scholar 

  3. EEC (1994) Council regulation (EEC) no. 2377/90. Amending regulation no. 1430/94 of 22 June 1994. Off J Eur Commun L156 23 (6)

  4. Impens S, Reybroeck W, Vercammen J, Courtheyn D, Ooghe S, DeWash K, Smedts W, DeBrabander H (2003) Anal Chim Acta 483:153–163

    Article  CAS  Google Scholar 

  5. Xie MX, Liu Y, Qiu YM, Han J, Liu YZ (2005) Chin J Anal Chem 33:1–4

    CAS  Google Scholar 

  6. Ortelli D, Edder P, Corvi C (2004) Chromatographia 59:61–64

    Article  CAS  Google Scholar 

  7. Grotewahl D (2006) Entwicklung von Methoden zur Bestimmung von Chloramphenicol in Bienenprodukten mittels LC–MS. Cuvillier, Göttingen

    Google Scholar 

  8. Nicolich RS, Werneck-Barroso E, Marques MAS (2006) Anal Chim Acta 565:97–102

    Article  CAS  Google Scholar 

  9. Sellergren B (2001) J Chromatogr A 906:227–252

    Article  CAS  Google Scholar 

  10. Ye L, Mosbach K (2001) J Incl Phenom Macrocycl Chem 41:107–113

    Article  CAS  Google Scholar 

  11. Cormack PAG, Elorza AZ (2004) J Chromatogr B 804:173–182

    Article  CAS  Google Scholar 

  12. Levi R, McNiven S, Piletsky SA, Cheong SH, Yano K, Karube I (1997) Anal Chem 69:2017–2021

    Article  CAS  Google Scholar 

  13. McNiven S, Kato M, Levi R, Yano K, Karube I (1998) Anal Chim Acta 365:69–74

    Article  CAS  Google Scholar 

  14. Suárez-Rodríguez JL, Díaz-García ME (2001) Biosens Bioelectron 16:955–961

    Article  Google Scholar 

  15. Schirmer C, Meisel H (2008) Anal Bioanal Chem 392:223–229

    Article  CAS  Google Scholar 

  16. Schirmer C, Meisel H (2006) J Chromatogr A 1132:325–328

    Article  CAS  Google Scholar 

  17. Mena ML, Agüí L, Martines-Ruiz P, Yáñez-Sedeño P, Reviejo AJ, Pingarrón JM (2003) Anal Bioanal Chem 376:18–25

    CAS  Google Scholar 

  18. Boyd B, Björk H, Billing J, Shimelis O, Axelsson S, Leonora M, Yilmaz E (2007) J Chromatogr A 1174:63–71

    Article  CAS  Google Scholar 

  19. Mohamed R, Richoz-Payot J, Gremaud E, Mottier P, Yilmaz E, Tabet JC, Guy PA (2007) Anal Chem 79:9557–9565

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Safety and Quality of Milk and Fish Products, Max Rubner-Institute, Federal Research Institute of Nutrition and Food, Hermann-Weigmann-Str. 1, 24103, Kiel, Germany

    Christina Schirmer & Hans Meisel

Authors
  1. Christina Schirmer
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  2. Hans Meisel
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Correspondence to Hans Meisel.

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Schirmer, C., Meisel, H. Chromatographic evaluation of polymers imprinted with analogs of chloramphenicol and application to selective solid-phase extraction. Anal Bioanal Chem 394, 2249–2255 (2009). https://doi.org/10.1007/s00216-009-2898-2

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  • DOI: https://doi.org/10.1007/s00216-009-2898-2

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