Abstract
A variety of bulk polymers for the selective separation of chloramphenicol were synthesised from 2-vinylpyridine, diethylaminoethyl methacrylate or methacrylic acid monomers. Chromatographic evaluation indicated that chloramphenicol was retained under nonpolar elution conditions (k = 58.65) through selective hydrogen bonding and ionic interactions. The retention of chloramphenicol under aqueous elution conditions (k > 100) results from nonselective hydrophobic interactions. Under nonpolar elution conditions, the functional monomer employed imparted a significant influence on the recognition properties of the corresponding polymer. After solid-phase extraction using a molecularly imprinted polymer as sorbent and either an organic or aqueous washing solvent, nearly 100% recovery from the chloramphenicol standard solution was achieved, and nearly 90% recovery could be attained from spiked honey samples. The molecularly imprinted polymer was well suited to suppress matrix effects, and provided optimal preconcentration of the target molecule (chloramphenicol) prior to chromatographic analysis.
Similar content being viewed by others
References
Yunis AA (1988) Ann Rev 28:83–100
Holt D, Harvey D, Hurley R (1993) Adverse Drug React Toxicol Rev 12:83–95
Council Regulation (EEC) No 2377/90, amending regulation No 1430/94 of 22 June 1994, Off J Eur Commun L156 23(6)
Commission Decision 2003/181/EC of 13 March 2003, amending decision 2002/657/, Off J Eur Commun L71/17
Akhtar MH, Danis C, Sauve A, Barry C (1995) J Chromatogr A 696:123–130
Pfenning AP, Roybal JE, Rupp HS, Turnipseed SB, Gonzales SA, Hurlbut JA (2000) J AOAC Int 83:26–30
Roudaut B (1996) J Liq Chromatogr R T 19:1097–1105
Boulaire SL, Bauduret JC, Andre F (1997) J Agric Food Chem 45:2134–2142
Abjean JP (1997) J AOAC Int 80:737–740
Impens S, Reybroeck W, Vercammen J, Courtheyn D, Ooghe S, DeWash K, Smedts W, DeBrabander H (2003) Anal Chim Acta 483:153–163
Scortichini G, Annunziata L, Haouet MN, Benedett F, Krusteva I, Galarini R (2005) Anal Chim Acta 535:43–48
Xie MX, Liu Y, Qiu YM, Han J, Liu YZ (2005) Chin J Anal Chem 33:1–4
Ortelli D, Edder P, Corvi C (2004) Chromatographia 59:61–64
Grotewahl D (2006) Entwicklung von Methoden zur Bestimmung von Chloramphenicol in Bienenprodukten mittels LC-MS. Cuvillier Verlag, Göttingen
Nicolich RS, Werneck-Barroso E, Marques MAS (2006) Anal Chim Acta 565:97–102
Sellergren B (2001) J Chromatogr A 906:227–252
Ye L, Mosbach K (2001) J Incl Phenom Macrocycl Chem 41:107–113
Cormack PAG, Elorza AZ (2004) J Chromatogr B 804:173–182
Mena ML, Agüí L, Martines-Ruiz P, Yáñez-Sedeño P, Reviejo AJ, Pingarrón JM (2003) Anal Bioanal Chem 376:18–25
Schirmer C, Meisel H (2006) J Chromatogr A 1132:325–328
Boyd B, Björk H, Billing J, Shimelis O, Axelsson S, Leonora M, Yilmaz E (2007) J Chromatogr A 1174:63–71
Mohamed R, Richoz-Payot J, Gremaud E, Mottier P, Yilmaz E, Tabet JC, Guy PA (2007) Anal Chem 79:9557–9565
Levi R, McNiven S, Piletsky SA, Cheong SH, Yano K, Karube I (1997) Anal Chem 69:2017–2021
McNiven S, Kato M, Levi R, Yano K, Karube I (1998) Anal Chim Acta 365:69–74
Suárez-Rodríguez JL, Díaz-García ME (2001) Biosens Bioelectron 16:955–961
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Schirmer, C., Meisel, H. Molecularly imprinted polymers for the selective solid-phase extraction of chloramphenicol. Anal Bioanal Chem 392, 223–229 (2008). https://doi.org/10.1007/s00216-008-2269-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-008-2269-4