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Determination of trace tetracycline antibiotics in foodstuffs by liquid chromatography–tandem mass spectrometry coupled with selective molecular-imprinted solid-phase extraction

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Abstract

A rapid, specific, and sensitive method has been developed using molecularly imprinted polymers (MIPs) as solid-phase extraction sorbents for extraction of trace tetracycline antibiotics (TCs) in foodstuffs. MIPs were prepared by precipitation polymerization using tetracycline as the template. Under the optimal condition, the imprinting factors for MIPs were 4.1 (oxytetracycline), 7.0 (tetracycline), 7.4 (chlortetracycline), 7.7 (doxycycline), respectively. Furthermore, the performance of MIPs as solid-phase extraction sorbents was evaluated and high extraction efficiency of molecularly imprinted solid-phase extraction (MISPE) procedure was demonstrated. Compared with commercial sorbents, MISPE gave a better cleanup efficiency than C18 cartridge and a higher recovery than Oasis HLB cartridge. Finally, the method of liquid chromatography–tandem mass spectrometry coupled with molecular-imprinted solid-phase extraction was validated in real samples including lobster, duck, honey, and egg. The spiked recoveries of TCs ranged from 94.51% to 103.0%. The limits of detection were in the range of 0.1–0.3 μg kg−1.

Chromatograms obtained by direct injection of the spiked egg extracts (5 × 10-3 mmol L−1) and purification with MISPE

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Acknowledgement

This work was supported by the National Nature Science Foundation of China (grant no. 20407009, 30771807) and the Entry–Exit Inspection and Quarantine Bureau of PRC (grant no. 2006IK152).

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Authors and Affiliations

  1. MOE Key Laboratory of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei, 430030, China

    Tao Jing, Xiao-Dan Gao, Yan Wang, Qiao-Lin Hao, Yi-Kai Zhou & Su-Rong Mei

  2. Hubei Entry–Exit Inspection and Quarantine Bureau of PRC, Wuhan, Hubei, 430022, China

    Peng Wang, Yan-Fei Lin & Xiao-Zhong Hu

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  1. Tao Jing
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  2. Xiao-Dan Gao
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  4. Yan Wang
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  5. Yan-Fei Lin
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Correspondence to Su-Rong Mei.

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Jing, T., Gao, XD., Wang, P. et al. Determination of trace tetracycline antibiotics in foodstuffs by liquid chromatography–tandem mass spectrometry coupled with selective molecular-imprinted solid-phase extraction. Anal Bioanal Chem 393, 2009–2018 (2009). https://doi.org/10.1007/s00216-009-2641-z

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  • DOI: https://doi.org/10.1007/s00216-009-2641-z

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