Abstract
Protein microarray technology has enormous potential for in vitro diagnostics (IVD). Miniaturized parallelized immunoassays are perfectly suited to generating a maximum of diagnostically relevant information from minute amounts of sample whilst only requiring small amounts of reagent. Protein microarrays have become well-established research tools in basic and applied research and the first products are already on the market. This article reviews the current state of protein microarrays and discusses developments and future demands relating to protein arrays in their role as multiplexed immunoassays in the field of diagnostics.
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Notes
Here “detection sensitivity” is used in terms of limit of detection, i.e. the smallest amount of analyte which can be detected with acceptable statistical significance. By contrast we use the term “assay sensitivity”, i.e. the smallest detectable difference in analyte concentration.
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Hartmann, M., Roeraade, J., Stoll, D. et al. Protein microarrays for diagnostic assays. Anal Bioanal Chem 393, 1407–1416 (2009). https://doi.org/10.1007/s00216-008-2379-z
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DOI: https://doi.org/10.1007/s00216-008-2379-z