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Comprehensive two-dimensional liquid chromatography in the analysis of antioxidant phenolic compounds in wines and juices

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Abstract

A novel comprehensive two-dimensional liquid chromatographic (LC×LC) system was developed for the quantification of antioxidant phenolic compounds in wine and juice. The system allows parts of the sample that are well separated in the first column to pass directly to the detector after the first column, while the rest of the sample proceeds to the second column. The optimised LC×LC system employed a combination of two C18 columns, the latter column with an ion-pair reagent (tetrapentylammonium bromide). The relative standard deviations (RSD) for the retention times were better than 0.01% in the first dimension and on average 6.3% in the second. The RSD values of the peak volumes varied from 3% (protocatechuic acid) to 13% (caffeic acid, n = 3, 10 μg/ml).

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Acknowledgement

Funding from the Academy of Finland (project no. 110429) is gratefully acknowledged.

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Authors and Affiliations

  1. Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, 00014, Helsinki, Finland

    Maarit Kivilompolo & Tuulia Hyötyläinen

  2. Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 61200, Brno, Czech Republic

    Vít Obůrka

Authors
  1. Maarit Kivilompolo
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  2. Vít Obůrka
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  3. Tuulia Hyötyläinen
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Correspondence to Tuulia Hyötyläinen.

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Kivilompolo, M., Obůrka, V. & Hyötyläinen, T. Comprehensive two-dimensional liquid chromatography in the analysis of antioxidant phenolic compounds in wines and juices. Anal Bioanal Chem 391, 373–380 (2008). https://doi.org/10.1007/s00216-008-1997-9

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  • DOI: https://doi.org/10.1007/s00216-008-1997-9

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