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Sensitive bioanalysis—combining single-molecule spectroscopy with mono-labeled self-quenching probes

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Abstract

Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled fluorescent probes that are quenched by photoinduced electron transfer (PET).

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Acknowledgement

The authors would like to thank Professor Dr Markus Sauer and Dr Hannes Neuweiler for providing Figs. 5 and 6 and Professor Dr Jürgen Wolfrum for his support. Julie Fiore is gratefully acknowledged for carefully reading the manuscript.

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Authors and Affiliations

  1. Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 229, 69120, Heidelberg, Germany

    Nicole Marmé

  2. Department of Functional Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany

    Jens-Peter Knemeyer

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  1. Nicole Marmé
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  2. Jens-Peter Knemeyer
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Correspondence to Nicole Marmé.

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Marmé, N., Knemeyer, JP. Sensitive bioanalysis—combining single-molecule spectroscopy with mono-labeled self-quenching probes. Anal Bioanal Chem 388, 1075–1085 (2007). https://doi.org/10.1007/s00216-007-1365-1

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  • DOI: https://doi.org/10.1007/s00216-007-1365-1

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