Abstract
A newly designed glass-PDMS microchip-based sensor for use in the determination of Ca2+ ions has been developed, utilizing reflectance measurements from arsenazo III (1,8-dihydroxynaphthalene-3,6-disulfonic acid-2,7-bis[(azo-2)-phenyl arsenic acid]) immobilized on the surface of polymer beads. The beads, produced from cross-linked poly(p-chloromethylstyrene) (PCMS), were covalently modified with polyethylenimine (PEI) to which the Arsenazo III could be adsorbed. The maximum amount of Arsenazo III which could be immobilized onto the PEI-attached PCMS beads was found to be 373.71 mg g−1 polymer at pH 1. Once fabricated, the beads were utilized at the detection point of the microfluidic sensor device with a fiber optic assembly for reflectance measurements. Samples were mobilized past the detection point in the sensor where they interact with the immobilized dye. The sensor could be regenerated and re-used by rinsing with HCl solution. The pH, voltage, linear range, and the effect of interfering ions were evaluated for Ca2+ determination using this microchip sensor. At the optimum potential, 0.8 kV, and pH 9.0, the linear range of the microchip sensor was 3.57 × 10−5 – 5.71 × 10−4 M Ca2+, with a limit of detection (LOD) of 2.68 × 10−5 M. The microchip biosensor was then applied for clinical analysis of calcium ions in serum with good results.
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Acknowledgements
The authors wish to thank Chemistry Departments of Hacettepe University, UVA, and TUBITAK (The Scientific and Technological Research Council of Turkey) for providing grant to this project.
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Caglar, P., Tuncel, S.A., Malcik, N. et al. A microchip sensor for calcium determination. Anal Bioanal Chem 386, 1303–1312 (2006). https://doi.org/10.1007/s00216-006-0776-8
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DOI: https://doi.org/10.1007/s00216-006-0776-8