Abstract
In this research, simultaneous quantification of cysteine (Cys) and histidine (His) using a paper-based sensor was investigated for the first time. A microfluidic paper-based sensor is a promising amino acid determination tool due to its low cost, low sample consumption, and fast analysis and is easy to make. We used SBA-15 as porous materials that are crystalline compounds formed with surfactants and TEOS as a silica source. The SBA-15 sample was characterized by X-ray diffraction, Fourier transform infrared, and scanning electron microscope techniques. The microfluidic paper-based sensors were fabricated using a wax pen, and the mesoporous SBA-15 modified filter paper. The fabricated sensors for Cys and His determination are operated based on an indicator-displacement assay. A comparative determination study of Cys and His on the sensors was carried out. The results illustrated that the addition of silica nanoporous material led to an immediate and uniform color change. The sensors were successfully exploited in the simultaneous determination of urinary Cys and His levels, thus providing the potential opportunity for clinical diagnosis. The linear range of 1.0 to 90.0 μM and 1.0 to 100 μM for His and Cys was obtained from the calibration data. The detection limits were also calculated (S/N = 3) for His and Cys of 0.5 μM and 1.5 μM, respectively. The proposed method showed a good agreement with the results achieved by a standard method.
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References
Allen MP (1995) ChemTrack Inc. U.S. Patent 5, pp 409–664
Bae DR, Han WS, Lim JM, Kang S, Lee JY, Kang D, Jung JH (2010) Lysine-functionalized silver nanoparticles for visual detection and separation of histidine and histidine-tagged proteins. Langmuir 26:2181–2185. https://doi.org/10.1021/la9026865
Bhakta SA, Borba R, Tabajr M, Garcia CD, Carrilho E (2014) Determination of nitrite in saliva using microfluidic paper-based analytical devices. Anal Chim Acta 809:117–122. https://doi.org/10.1016/j.aca.2013
Buryak A, Severin K (2005) A chemosensor array for the colorimetric identification of 20 natural amino acids. J Am Chem Soc 127:3700–3701. https://doi.org/10.1021/ja042363v
Cao W, Jiao Q, Fu Y, Chen L, Liu Q (2003) Mechanism of the interaction between bromophenol blue and bovine serum albumin. Spectrosc Lett 36:197–209. https://doi.org/10.1081/SL-120024351
Carrilho E, Martinez AW, Whitesides GM (2009) Understanding wax printing: a simplemicropatterning process for paper-based microfluidics. Anal Chem 16:7091–7095. https://doi.org/10.1021/ac901071p
Cate DM, Dungchai W, Cunningham JC, Volckens J, Henry CS (2013) Simple distance-based measurement for paper analytical devices. Lab Chip 13:2397–2404. https://doi.org/10.1039/c3lc50072a
Chen X, Chen J, Wang F, Xiang X, Luo M, Ji X, He Z (2012) Determination of glucose and uric acid with bienzymecolorimetry on microfluidic paper-based analysis devices. Biosens Bioelectron 35:363–368. https://doi.org/10.1016/j.bios.2012.03.018
Dhar GM, Kumaran GM, Kumar M, Rawat KS, Sharma LD, Raju BD, RaoK SR (2005) Physico-chemical characterization and catalysis on SBA-15 supported molybdenum hydrotreating catalysts. Catal Today 99:309–314. https://doi.org/10.1016/j.cattod.2004.10.005
Dungchai W, Chailapakul O, Henry CS (2010) Use of multiple colorimetric indicators for paper-based microfluidic devices. Anal Chim Acta 674:227–233. https://doi.org/10.1016/j.aca.2010.06.019
Dungchai W, Chailapakul O, Henry CS (2013) A microfluidic paper-based analytical device for rapid quantification of particulate chromium. Anal Chim Acta 800:50–55. https://doi.org/10.1016/j.aca.2013.09.008
Ellerbee AK, Phillips ST, Siegel AC, Mirica KA, Martinez AW, Strieh LP, Jain N, Prentiss M, Whitesides GM (2009) Quantifying colorimetric assays in paper-based microfluidic devices by measuring the transmission of light through paper. Anal Chem 81:8447–8452. https://doi.org/10.1021/ac901307q
Evans E, Gabriel EFM, Benavidez TE, Karlos W, Coltrob T, Garcia CD (2014) Modification of microfluidic paper-based devices with silica nanoparticles. Analyst 139:5560–5567. https://doi.org/10.1039/c4an01147c
Fekkes D (1996) State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples. J Chromatogr B Biomed Sci Appl 682:3–22. https://doi.org/10.1016/0378-4347(96)00057-6
Folmer-Andersen JF, Kitamura M, Anslyn EV (2006) Pattern-based discrimination of enantiomeric and structurally similar amino acids: an optical mimic of the mammalian taste response. J Am Chem Soc 128:5652–5653. https://doi.org/10.1021/ja061313i
Ge L, Wang S, Song X, Ge YuS (2012) 3D Origami-based multifunction-integrated immune device: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device. Lab Chip 12:3150–3158. https://doi.org/10.1039/c2lc40325k
Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM (1997) Microchip device for performing enzyme assays. Anal Chem 69:3407–3412. https://doi.org/10.1021/ac970192p
Hardman JD, Slater JH, Reid AG, Lang WK, Jackson JR (2003) Diamatrix Ltd. U.S. Patent 6, vol 573, p 108
Herzenberg LA, De Rosa SC, Dubs JG, Roederer M, Anderson MT, Ela SW, Deresinski SC (1997) Glutathione deficiency is associated with impaired survival in HIV disease. Proc Natl Acad Sci USA 94:1967–1972. https://doi.org/10.1073/pnas.94.5.1967
Hossain SMZ, LuckhamFadden REM, Brennan MJ (2009) Reagent less bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. Anal Chem 81:9055–9064. https://doi.org/10.1021/ac901714h
Khajeh Sharifi H, Sheini A (2014) a selective naked-eye detection and determination of cysteine using an indicator-displacement assay in urine sample. Sens Actuat B199:457–462. https://doi.org/10.1016/j.snb.2014.03.055
KhajehSharifi H, TeimouriFard Z (2016) The design of a new chemosensor for qualitative and quantitive determination of Histidine in some real samples. http://ganj-old.irandoc.ac.ir/articles/969987
Kim YS, Park JG, Lee SA, Kim C (2015) A colorimetric chemosensor for the sequential detection of copper ion and amino acids (cysteine and histidine) in aqueous solution. RSC Adv 5:31179–31188. https://doi.org/10.1039/C5RA00544B
Klasner SA, Price AK, Hoeman KW, Wilson RS, Bell KJ, Culbertson CT (2010) Paper-based microfluidic devices for analysis of clinically relevant analytes present in urine and saliva. Anal Bioanal Chem 397:1821–1829. https://doi.org/10.1007/s00216-010-3718-4
Lankelma J, Nie Z, Carrilho E, Whitesides GM (2012) Paper-based analytical device for electrochemical flow-injection analysis of glucose in urine. Anal Chem 84:4147–4152. https://doi.org/10.1021/ac3003648
Leung D, Folmer-Andersen JF, Lynch VM, Anslyn EV (2008) Using enantio selective indicator displacement assays to determine the enantiomeric excess of α-amino acids. J Am Chem Soc 130:12318–12327. https://doi.org/10.1021/ja803806c
Li X, Ballerini DR, Shen W (2012) A perspective on paper-based microfluidics: Current status and future trend. Bio Microfluidics 6:11301–1130113. https://doi.org/10.1063/1.3687398
Lu Y, Shi WW, Jiang L, Qin JH, Lin BC (2009) Rapid prototyping of paper-based microfluidics with wax for low-cost, portable bioassay. Electrophoresis 30:1497–1500. https://doi.org/10.1002/elps.200800563
Mao HB, Cremer PS, Manson MD (2003) A sensitive, versatile microfluidic assay for bacterial chemotaxis. Proc Natl Acad Sci USA 100:5449–5454. https://doi.org/10.1073/pnas.0931258100
Martinez AW, Phillips ST, Butte MJ, Whitesides GM (2007) Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew Chem Int Ed 46:1318–1320. https://doi.org/10.1002/anie.200603817
Martinez AW, Phillips ST, Carrilho E, Thomas SW, Sindi H, Whitesides GM (2008) Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real-time, off-site diagnosis. Anal Chem 80:3699–3707. https://doi.org/10.1021/ac800112r
Martinez AW, Phillips ST, Whitesides GM, Carrilho E (2010) Diagnostics for the developing world: microfluidic paper-based analytical devices. Anal Chem 82:3–10. https://doi.org/10.1021/ac9013989
Muthu Kumaran G, Garg S, Soni K, Kumar M, Sharma LD, Murali Dhar G, Rama Rao KS (2006) Effect of Al-SBA-15 support on catalytic functionalities of hydrotreating catalysts: I. Effect of variation of Si/Al ratio on catalytic functionalities. Appl Catal A 305:123–129. https://doi.org/10.1016/j.apcata.2006.02.057
Nakanishi K, Sakiyama T, Imamura K (2001) on the adsorption of proteins on solid surfaces, a common but very complicated phenomenon. J Biosci Bioeng 91:233–244. https://doi.org/10.1016/S1389-1723(01)80127-4
Nery EW, Kubota LT (2013) Sensing approaches on paper-based devices: a review. Anal Bioanal Chem 405:7573–7595. https://doi.org/10.1007/s00216-013-6911-4
Nie Z, Nijhuis CA, Gong J, Chen X, Kumachev A, Martinez AW, Narovlyansky M, Whitesides GM (2010) Electrochemical sensing in paper-based microfluidic devices. Lab Chip 10:477–483. https://doi.org/10.1039/b917150a
Nie J, Liang Y, Zhang Y, Le S, Li D, Zhang S (2013) One-step patterning of hollow microstructures in paper by laser cutting to create microfluidic analytical devices. Analyst 138:671–676. https://doi.org/10.1039/C2AN36219H
Pan JZ, Yao B, Fang Q (2010) Hand-held photometer based on liquid-core waveguide absorption detection for nanoliter-scale samples. Anal Chem 82:3394–3398. https://doi.org/10.1021/acs.analchem.7b04031
Patel G, Menon S (2009) Recognition of lysine, arginine, and histidine by novel p-sulfonatocalix[4]arenethiol functionalized gold nanoparticles in aqueous solution. Chem Commun. https://doi.org/10.1039/B905141D
Sabel CE, Neureuther JM, Siemann S (2010) A spectrophotometric method for the determination of zinc, copper, and cobalt ions in metalloproteins using Zincon. Anal Biochem 397:218–226. https://doi.org/10.1016/j.ab.2009.10.037
Shahrokhian S (2001) Lead phthalocyanine as a selective carrier for preparation of a cysteine-selective electrode. Anal Chem 73:5972–5978. https://doi.org/10.1021/ac010541m
Thom NK, Lewis GG, DiTucci MJ, Phillips ST (2013) Two general designs for fluidic batteries in paper-based microfluidic devices that provide predictable and tunable sources of power for on-chip assays. RSC Adv 3:6888–6895. https://doi.org/10.1039/C3RA40701B
Tomazelli Coltro WK, Cheng CM, Carrilho E, de Jesus DP (2014) Recent advances in low-cost microfluidic platforms for diagnostic applications. Electrophoresis 35:2309–2324. https://doi.org/10.1002/elps.201400006
Wada AM, Tucker HN (1998) Antioxidant characteristics of L-histidine. J Nutr Biochem 9:308–315. https://doi.org/10.1016/S0955-2863(98)00022-9
Xu LQ, Neoh KG, Kang ET, Fu GD (2013) Rhodamine derivative-modified filter papers for colorimetric and fluorescent detection of Hg2+ in aqueous media. J Mater Chem A 1:2526–2532. https://doi.org/10.1039/C2TA01072K
Xiong L, Peng H, Rao H, Liu X, Lu X (2018) A selective colorimetric sensing strategy for cysteine based on an indicator-displacement mechanism. New J Chem 42:4324–4330. https://doi.org/10.1039/C7NJ03887A
Xue Z, Xiong L, Rao H, Liu X, Lu X (2019)A naked-eye liquid-phase colorimetric assay of simultaneous detect cysteine and lysine. Dyes Pigments 160: 151–158.https://doi.org/10.1016/j.dyepig.2018.07.054
Yetisen AK, Akram MS, Lowe CR (2013) Paper-based microfluidic point-of-care diagnostic devices. Lab Chip 13:2210–2251. https://doi.org/10.1039/c3lc50169h
Yu J, Ge L, Huang J, Wang S, Ge S (2011) Microfluidic paper-based chemiluminescence biosensor for simultaneous determination of glucose and uric acid. Lab Chip 11:1286–1291. https://doi.org/10.1039/c0lc00524j
Zhang H, Sun J, Ma D, Weinberg G, Su DS, Bao X (2006) Engineered complex emulsion system: toward modulating pore length and morphological architecture of mesoporous silicas. J Phys Chem B 110:25908–25915. https://doi.org/10.1021/jp065760w
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Razavi, F., Khajehsharifi, H. A colorimetric paper-based sensor with nanoporous SBA-15 for simultaneous determination of histidine and cysteine in urine samples. Chem. Pap. 75, 3401–3410 (2021). https://doi.org/10.1007/s11696-021-01548-4
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DOI: https://doi.org/10.1007/s11696-021-01548-4