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Separation and identification of compounds in Adinandra nitida by comprehensive two-dimensional liquid chromatography coupled to atmospheric pressure chemical ionization source ion trap tandem mass spectrometry

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Abstract

A comprehensive two-dimensional liquid chromatographic (2D-LC) separation system based on the combination of a CN column and a Merck Chromolith Flash reversed-phase column was developed for the separation of components in Adinandra nitida, one type of traditional Chinese medicine (TCM). The two dimensions were connected by a ten-port, dual-position valve controlled automatically by software written in-house. The effluents were detected by both ultraviolet and atmospheric pressure chemical ionization source ion trap tandem mass spectrometry (MS). The calculated peak capacity of the 2D-LC–MS/MS system was above 1240. More than 57 components were resolved in the methanol extract from Adinandra nitida leaves, and five of these were identified based on their relative retention times, molecular weights and MS/MS spectra.

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Acknowledgements

The financial support from the National Natural Science Foundation of China (No. 20375040) and the Chinese Academy of Sciences (No. KGCX2-SW-213) is gratefully acknowledged.

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Authors and Affiliations

  1. National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, People’s Republic of China

    Jie Zhang, Dingyin Tao, Jicheng Duan, Zhen Liang, Weibing Zhang, Lihua Zhang & Yukui Zhang

  2. Health Science Center at San Antonio, The University of Texas, 78229-3900, San Antonio, TX, USA

    Yushu Huo

Authors
  1. Jie Zhang
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  2. Dingyin Tao
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  3. Jicheng Duan
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  4. Zhen Liang
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  5. Weibing Zhang
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  6. Lihua Zhang
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  7. Yushu Huo
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  8. Yukui Zhang
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Correspondence to Lihua Zhang.

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Zhang, J., Tao, D., Duan, J. et al. Separation and identification of compounds in Adinandra nitida by comprehensive two-dimensional liquid chromatography coupled to atmospheric pressure chemical ionization source ion trap tandem mass spectrometry. Anal Bioanal Chem 386, 586–593 (2006). https://doi.org/10.1007/s00216-006-0539-6

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  • DOI: https://doi.org/10.1007/s00216-006-0539-6

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