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Affinity-based mass spectrometry using magnetic iron oxide particles as the matrix and concentrating probes for SALDI MS analysis of peptides and proteins

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Abstract

Silane-immobilized magnetic iron oxide particles were used as the assisting material in surface-assisted laser desorption/ionization (SALDI) mass spectrometric analysis. This approach can be used to analyze small proteins and peptides. The upper detectable mass range is approximately 16 kDa. The detection limit for peptides is about 20 fmol. Silanized iron oxide particles with negatively charged functionalities can also be used as the affinity probes to selectively trap oppositely charged species from sample solutions by adjusting the pH of the solution. A tryptic digest product of cytochrome C at a concentration as low as 10 nM can be enriched by the particles and directly analyzed by iron oxide SALDI MS without the need for elution steps. Affinity-based mass spectrometry using the bifunctional silanized magnetic iron oxide particles as the SALDI matrix and concentrating probe is demonstrated in this study.

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Acknowledgements

The authors would like to thank the National Science Council of Taiwan for financial support.

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Authors and Affiliations

  1. Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 300, Taiwan

    Wei-Yu Chen & Yu-Chie Chen

  2. Institute of Molecular Science, National Chiao Tung University, Hsinchu, 300, Taiwan

    Yu-Chie Chen

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  1. Wei-Yu Chen
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  2. Yu-Chie Chen
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Correspondence to Yu-Chie Chen.

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Chen, WY., Chen, YC. Affinity-based mass spectrometry using magnetic iron oxide particles as the matrix and concentrating probes for SALDI MS analysis of peptides and proteins. Anal Bioanal Chem 386, 699–704 (2006). https://doi.org/10.1007/s00216-006-0427-0

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  • DOI: https://doi.org/10.1007/s00216-006-0427-0

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