Skip to main content
SpringerLink
Log in

Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Fumonisins B1 (FB1) and fumonisin B2 (FB2) are the main members of a family of mycotoxins produced by Fusarium verticillioides, Fusarium proliferatum, and other fungi species of the section Liseola. The present work shows the results of comparative studies using two different procedures for the analysis of fumonisins in maize and maize-based samples. The studied analytical methods involve extraction with methanol/water, dilution with PBS, and clean-up through immunoaffinity columns. Two reagents (o-phthaldialdehyde and naphthalene-2,3-dicarboxaldehyde) were studied for formation of fluorescent derivatives. The separation and identification were carried out by high-performance liquid chromatography with fluorescence detection. The optimized method for analysis of fumonisins in maize involved extraction with methanol/water (80:20), clean-up with an immunoaffinity column, and derivatization with naphthalene-2,3-dicarboxaldehyde (NDA). The limit of detection was 20 μg kg−1 for FB1 and 15 μg kg−1 for FB2. Recoveries of FB1 and FB2 ranged from 79% to 99.6% for maize fortified at 150 μg kg−1 and 200 μg kg−1, respectively, with within-day RSDs of 3.0 and 2.7%. The proposed method was applied to 31 samples, and the presence of fumonisins was found in 14 samples at concentrations ranging from 113 to 2,026 μg kg−1. The estimated daily intake of fumonisins was 0.14 μg kg−1 body weight per day.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Lerda D, Bistoni BM, Peralta N, Ychari S, Vazquez M, Bosio G (2005) Food Chem Toxicol 43:691–698

    Article  PubMed  CAS  Google Scholar 

  2. Thiel PG, Marasas WFO, Sydenham EW, Shephard GS, Gelderblom WCA., Nieuwenhuis JJ (1991) Appl Environ Microbiol 57:1089–1093

    PubMed  CAS  Google Scholar 

  3. Labuda R, Tanèinova D, Hudec K (2003) Ann Agric Environ Med 10:61–66

    PubMed  Google Scholar 

  4. Stack ME (1998) J AOAC Int 81:737–740

    PubMed  CAS  Google Scholar 

  5. IARC (2002) IARC monographs on the evaluation of carcinogenic risks to humans, Lyon, France, 82:301–366

  6. Bolger M, Coker RD, DiNovi M, Gaylor D, Gelderblom W, Olsen M, Paster N, Riley RT, Shephard G, Speijers (2001) WHO food additives series 47, FAO food and nutrition paper 74; prepared by the 56th meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA); WHO: Geneva, Switzerland, pp 103–279. Available at http://www.inchem.org/documents/jecfa/jecmono/v47je03.htm#6.0. Accessed October 2005

  7. Zinedine A, Brera C, Elakhdari S, Catano C, Debegnach F, Angelini S, Santis BD, Faid M, Benlemlih M, Minardi V, Miraglia M (2005) Food Control (in press)

  8. Bittencourt ABF, Oliveira CAF, Dilkin P, Corrêa B (2005) Food Control 16:117–120

    Article  CAS  Google Scholar 

  9. Bankole SA, Mabekoje OO (2004) Food Addit Contam 21:251–255

    Article  PubMed  CAS  Google Scholar 

  10. Sydenham EW, Gelderblom WCA, Thiel PG, Marasas WFO (1990) J Agric Food Chem 38:285–290

    Article  CAS  Google Scholar 

  11. Scudamore KA, Patel S (2000) Food Addit Contam 17:407–416

    Article  PubMed  CAS  Google Scholar 

  12. FAO (2003) Available at http://nue.okstate.edu/Crop_Information/World_Wheat_Production.htm. Accessed June 2005

  13. Doko MB, Rapior S, Visconti A, Schjøth JE (1995) J Agric Chem 43:429–434

    Article  CAS  Google Scholar 

  14. Duncan K, Kruger S, Zabe N, Kohn B, Prioli R (1998) J Chromatogr A 815:41–47

    Article  PubMed  CAS  Google Scholar 

  15. Lino CM, Silva LJG, Pena AS. Química Nova (in press)

  16. Scudamore KA, Nawaz S, Hetmanski MT (1997) Food Addit Contam 14:175–186

    PubMed  CAS  Google Scholar 

  17. Cortez-Rocha MO, Ramírez-Astudillo WR, Sánchez-Mariñez RI, Rosas-Burgos EC, Wong-Corral FJ, Borboa-Flores J, Castillón-Campaña LG, Tequida-Meneses M (2003) Bull Environ Contam Toxicol 70:668–673

    Article  PubMed  CAS  Google Scholar 

  18. Voss KA, Riley RT, Norred WP, Bacon CW, Meredith FI, Howard PC, Plattner RD, Collins TFX, Hansen DK, Porter JK (2001) Environ Health Perspectives 109 (suppl 2):259–266

    Article  CAS  Google Scholar 

  19. Lombaert GA, Pellaers P, Roscoe V, Mankotia M, Neil R, Scott PM (2003) Food Addit Contam 20:494–504

    Article  PubMed  CAS  Google Scholar 

  20. Dombrink-Kurtzman MA, Dvorak TJ (1999) J Agric Food Chem 47:622–627

    Article  PubMed  CAS  Google Scholar 

  21. Castro MFPM, Shephard GS, Sewram V, Vicente E, Mendonça TA, Jordan AC (2004) Food Addit Contam 21:693–699

    Article  PubMed  Google Scholar 

  22. Kim E-K, Scott PM, Lau BP-Y (2003) Food Addit Contam 20:161–169

    Article  PubMed  CAS  Google Scholar 

  23. Scott PM, Lawrence GA (1995) J Food Protection 58:1379–1382

    CAS  Google Scholar 

  24. Scott PM, Lawrence GA (1992) J AOAC Int 75:829–834

    CAS  Google Scholar 

  25. Molinié A, Faucet V, Castegnaro M, Pfohl-Leskowicz A (2005) Food Chem 92:391–400

    Article  Google Scholar 

  26. Cirillo T, Ritieni A, Galvano F, Cocchieri RA (2003) Food Addit Contam 20:566–571

    Article  PubMed  CAS  Google Scholar 

  27. Plattner RD (1999) Nat Toxins 7:365–370

    Article  PubMed  CAS  Google Scholar 

  28. Musser SM, Plattner RD (1997) J Agric Food Chem 45:1169–1173

    Article  CAS  Google Scholar 

  29. Bennett GA, Richard JL (1994) J AOAC Int 77:501–506

    CAS  Google Scholar 

  30. Shephard GS (1998) J Chromatogr A 815:31–39

    Article  PubMed  CAS  Google Scholar 

  31. Williams LD, Meredith FI, Riley RT (2004) J Chromatogr B 806:311–314

    Article  CAS  Google Scholar 

  32. Chu FS, Li GY (1994) Appl Environ Microbiol 60:847–852

    PubMed  CAS  Google Scholar 

  33. Sydenham EW, Shephard GS, Thiel PG (1992) J AOAC Int 75:313–318

    CAS  Google Scholar 

  34. Solfrizzo M, Girolamo A, Visconti A (2001) Food Addit Contam 18:227–235

    Article  PubMed  CAS  Google Scholar 

  35. Domijan AM, Peraica M, Jurjevic Z, Ivic D, Cvjetkovic B (2005) Food Addit Contam 22:677–680

    PubMed  CAS  Google Scholar 

  36. Castellá G, Bragulat MR, Cabañes FJ (1999) J Agric Food Chem 47:4707–4710

    Article  PubMed  Google Scholar 

  37. Broggi LE, Resnik SL, Pacin AM, González HHL, Cano G, Taglieri D (2002) Food Addit Contam 19:465–469

    Article  PubMed  CAS  Google Scholar 

  38. Piñeiro MS, Silva GE, Scott PM, Lawrence GA, Stack ME (1997) J AOAC Int 80:825–828

    PubMed  Google Scholar 

  39. Pittet A, Parisod V, Schellenberg M (1992) J Agric Food Chem 40:1352–1354

    Article  CAS  Google Scholar 

  40. Truckess MW, Stack ME, Allen S, Barrion N (1995) J AOAC Int 78:705–710

    Google Scholar 

  41. EMAN (2000). European Mycotoxin Awareness Network. Available at http://www.mycotoxins.org. Accessed April 2004

  42. Shephard GS, Marasas WFO, Leggott NL, Yasdanpanah H, Rahimian H, Safavi N (2000) J Agric Food Chem 48:1860–1864

    Article  PubMed  CAS  Google Scholar 

  43. INE (2004) Available at http://www.ine.pt. Accessed June 2005

  44. Commission Regulation (EC) N°856/2005. Official Journal of European Communities n°L 143, June 7 2005, pp 0003–0008

  45. Soriano JM, Dragacci S (2004) Food Res Int 37:367–374

    Article  CAS  Google Scholar 

  46. Gelderblom WCA, Marasas WF, Vleggaar R, Thiel PG, Cawood ME (1992) Mycopathologia 117:11–16

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was carried out with the financial support, which is gratefully acknowledged, from the FCT and FEDER/ POCTI. The authors are also gratefully recognized to FCT for a PhD fellowship granted to Liliana Silva.

Author information

Authors and Affiliations

  1. Group of Bromatology - CEF, Faculty of Pharmacy, University of Coimbra, 3000-295, Coimbra, Portugal

    C. M. Lino, L. J. G. Silva, A. L. S. Pena & M. I. Silveira

Authors
  1. C. M. Lino
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. J. G. Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. L. S. Pena
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. I. Silveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. M. Lino.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lino, C.M., Silva, L.J.G., Pena, A.L.S. et al. Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection. Anal Bioanal Chem 384, 1214–1220 (2006). https://doi.org/10.1007/s00216-005-0295-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-005-0295-z

Keywords

Search

Navigation