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Extraction without Organic Solvents in the Determination of Fumonisins B1, B2, and B3 in Maize by HPLC–FLD and ELISA Tests

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Abstract

The methods for the determination of fumonisins encompass several essential steps that are of great importance for obtaining accurate and reliable results. Extraction, as the first step in the sample preparation, is crucial for a satisfactory recovery. In this study, extraction methods without organic solvents were applied for the quantification of fumonisins B1 (FB1), B2 (FB2), and B3 (FB3) by high-performance liquid chromatography (HPLC) with fluorescence detector (FLD) and of total fumonisins by enzyme-linked immunosorbent assay (ELISA). The recoveries of the HPLC–FLD determination of two reference materials after extraction with distilled water were 99 ± 5.6 and 86 ± 3.9 % for FB1, and 111 ± 5 and 81 ± 1 % for FB2, while the extraction with phosphate buffer (PB) resulted in the recoveries of 104 ± 20.2 and 92 ± 8.6 %; for FB1 and 149 ± 13 and 100 ± 5.8 % for FB2. For FB3, the recovery with distilled water was 118 ± 0.5 % and with PB 131 ± 8.8 %. Two ELISA methods gave the following recoveries for total fumonisins: 100 ± 0.6 and 133 ± 0.7 % after the extraction with distilled water and 92 ± 2.2 and 123 ± 5.4 % with PB. The limits of detection and quantification using inorganic solvents and the HPLC–FLD method were at the level of micrograms per kilogram for all fumonisins, and somewhat higher than those obtained using organic extraction methods. The determination of individual and total fumonisins in the reference material by applying extraction with inorganic solvents revealed no significant difference (p > 0.05) as compared to the AOAC method and methods recommended by ELISA manufacturers. Generally, the extraction using inorganic solvents proved to be very effective for all the types of real, naturally contaminated samples.

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Acknowledgments

The work was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project No 172042).

Conflict of Interest

Sandra Jakšić declares that she has no conflict of interest.

Milica Živkov-Baloš declares that she has no conflict of interest.

Željko Mihaljev declares that he has no conflict of interest.

Zoran Mašić declares that he has no conflict of interest.

Igor Jajić declares that he has no conflict of interest.

Nemanja Banić declares that he has no conflict of interest.

Biljana Abramović declares that she has no conflict of interest.

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Scientific Veterinary Institute “Novi Sad”, Rumenački put 20, 21000, Novi Sad, Serbia

    Sandra Jakšić, Milica Živkov-Baloš, Željko Mihaljev & Zoran Mašić

  2. Department of Animal Science, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000, Novi Sad, Serbia

    Igor Jajić

  3. Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 3, 21000, Novi Sad, Serbia

    Nemanja Banić & Biljana Abramović

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  1. Sandra Jakšić
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  2. Milica Živkov-Baloš
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  3. Željko Mihaljev
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Correspondence to Biljana Abramović.

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Jakšić, S., Živkov-Baloš, M., Mihaljev, Ž. et al. Extraction without Organic Solvents in the Determination of Fumonisins B1, B2, and B3 in Maize by HPLC–FLD and ELISA Tests. Food Anal. Methods 8, 1446–1455 (2015). https://doi.org/10.1007/s12161-014-0030-5

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