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Quantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flour

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Abstract

Roundup Ready soybean powder has been subjected to different amounts of DNA fragmentation to assess the accuracy of real-time PCR on processed food. Certified reference material (CRM) containing 10 g kg−1 of Roundup Ready soybean (ERM-BF410d) prepared by a dry-mixing processing method was exposed to water at two temperatures, using three different mixing devices, or to baking temperature (250°C) for 30 min. The amount of DNA extracted from the different samples was quantified by fluorimetry. The amount of fragmentation of the extracted DNA was characterised by gel and capillary electrophoresis and the percentage of genetically modified (GM) soybean was determined by a double quantitative real-time PCR method. Measurement of the event GTS 40-3-2 (RUR) was possible in all the treated materials, because small amplicons were amplified. Correct RUR percentages could be measured for intact powders with little or no DNA fragmentation. For samples with a high level of DNA degradation, however, the accuracy of the measurement was found to depend on the method used for DNA extraction. Genomic DNA isolated by use of silica resin resulted in statistically significant overestimation of the amount of GM.

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Acknowledgements

We thank Isabelle Taverniers (CLO, Belgium), for performing some initial PCR analysis with RRS event-specific primers, and Esther Kock from the RIKILT Institute for Food Safety (The Netherlands), for performing external RUR analysis.

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Authors and Affiliations

  1. Institute for Reference Materials and Measurements, European Commission, Joint Research Centre, Retieseweg 111, 2440, Geel, Belgium

    Philippe Corbisier, Stefanie Trapmann, David Gancberg, Liesbeth Hannes, Pierre Van Iwaarden, Heinz Schimmel & Hendrik Emons

  2. Quality Department of Agro-food Products, Walloon Agricultural Research Centre, Chaussée de Namur 24, 5030, Gembloux, Belgium

    Gilbert Berben

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  1. Philippe Corbisier
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  2. Stefanie Trapmann
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Correspondence to Philippe Corbisier.

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Corbisier, P., Trapmann, S., Gancberg, D. et al. Quantitative determination of Roundup Ready soybean (Glycine max) extracted from highly processed flour. Anal Bioanal Chem 383, 282–290 (2005). https://doi.org/10.1007/s00216-005-0013-x

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  • DOI: https://doi.org/10.1007/s00216-005-0013-x

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