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Efficient DNA Extraction Procedures for Processed Food Derivatives—a Critical Step to Ensure Quality for GMO Analysis

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Abstract

Extraction of DNA is a crucial step for detection tests of genetically modified organisms (GMOs) in food derivatives. Quality of DNA needs to be ensured employing suitable extraction procedures as DNA amplifiability may be hampered by processing or presence of inhibitors due to complex constitution. In this study, procedures are being reported, which were found suitable to extract detectable amount of DNA from food derivatives including oil (canola, cottonseed, mustard, soybean), and other products of apple (juice, green apple), corn (popcorn, flakes, soup, flour, biscuits, puff corn, packed corn, baby corn, milk powder with corn oil, infant cereal), potato (chips), soy (chunks, sticks, milk, tofu), and tomato (soup, sauce). Food matrix was selected on the basis of globally approved genetically modified (GM) events of different crops. Amplifiability of DNA was ensured using endogenous gene-specific assays or/and inhibition test. The DNA extracts were of acceptable quality, which did not show any inhibition up to dilution series of 0.01–1.0 to 100 ng depending upon the type of food matrix. The procedures demonstrated herein could be efficiently employed for GMO testing for traceability or labelling of the product as well as testing for food authentication by food testing or processing laboratories.

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Abbreviations

CTAB:

Cetyltrimethylammonium bromide

DNA:

Deoxyribonucleic acid

E:

Efficiency

GM:

Genetically modified

GMO:

Genetically modified organism

PCR:

Polymerase chain reaction

R2 :

Correlation coefficient

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Acknowledgements

The authors thank the Indian Council of Agricultural Research (ICAR) and the Director, ICAR-National Bureau of Plant Genetic Resources for providing necessary facilities. The grant by the Department of Biotechnology (DBT) is duly acknowledged.

Funding

ICAR, ICAR-NBPGR, DBT.

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Authors and Affiliations

  1. Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India

    Monika Singh, Kushaldeep Kaur Sodhi, Aparna Paliwal, Shilpi Sharma & Gurinderjit Randhawa

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  1. Monika Singh
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  2. Kushaldeep Kaur Sodhi
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  3. Aparna Paliwal
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  4. Shilpi Sharma
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  5. Gurinderjit Randhawa
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Correspondence to Monika Singh.

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Monika Singh declares that she has no conflict of interest. Kushaldeep Kaur Sodhi declares that she has no conflict of interest. Aparna Paliwal declares that she has no conflict of interest. Shilpi Sharma declares that she has no conflict of interest. Gurinderjit Randhawa declares that she has no conflict of interest.

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Singh, M., Sodhi, K.K., Paliwal, A. et al. Efficient DNA Extraction Procedures for Processed Food Derivatives—a Critical Step to Ensure Quality for GMO Analysis. Food Anal. Methods 14, 2249–2261 (2021). https://doi.org/10.1007/s12161-021-02051-y

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