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Determination of ochratoxin A in pig tissues by liquid–liquid extraction and clean-up and high-performance liquid chromatography

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Abstract

A fast, simple, and sensitive HPLC–FD method is described for determination of ochratoxin A (OTA) in pig kidney and muscle; a small mass (<2.5 g) of sample and a relatively small volume (<15 mL) of a non-halogenated extraction solvent are required. Ochratoxin B, systematically absent from all the samples investigated, was used as internal standard. Liquid–liquid partition was used for sample clean-up. Recoveries at the 1 ng g−1 level were 86±15% and 74±8% for kidney and muscle, respectively, and detection limits were 0.14 and 0.15 ng g−1. Clean-up by solid-phase extraction (SPE) is required for pig liver. A survey of the OTA content of tissues of pigs slaughtered in southern Italy revealed that 52 out of 54 analysed samples were contaminated; the OTA concentration in kidney ranged between 0.26 and 3.05 ng g−1. The effect of measurement precision on compliance with legal limits is also discussed.

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Aknowledgements

Work was carried out with financial support from the University of Bari. S. Giacummo is acknowledged for his skilled help.

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Authors and Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Bari, Via Orabona 4, 70126, Bari, Italy

    L. Monaci & F. Palmisano

  2. Dipartimento di Sanità e Benessere degli Animali, Università degli Studi di Bari, Strada provinciale per Casamassima km. 3, 70010, Valenzano, Italy

    G. Tantillo

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  1. L. Monaci
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  2. G. Tantillo
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  3. F. Palmisano
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Correspondence to F. Palmisano.

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Monaci, L., Tantillo, G. & Palmisano, F. Determination of ochratoxin A in pig tissues by liquid–liquid extraction and clean-up and high-performance liquid chromatography. Anal Bioanal Chem 378, 1777–1782 (2004). https://doi.org/10.1007/s00216-004-2497-1

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  • DOI: https://doi.org/10.1007/s00216-004-2497-1

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