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Kinetic method for acetylsalicylic acid determination based on its inhibitory effect upon the catalytic decomposition of H2O2

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Abstract

The catalytic reaction of catalase was investigated, by means of a Clark oxygen sensor, in the presence of various concentrations of acetylsalicylic acid. Michaelis-Menten kinetic parameters were determined from Lineweaver-Burk plots, obtained in the absence and in the presence of the inhibitor. The inhibition pattern, suggested by the Lineweave-Burk plots, corresponds to a fully mixed inhibition mechanism. A kinetic method, based on the indicator reaction: \({\text{H}}_{{\text{2}}} {\text{O}}_{{\text{2}}} \xrightarrow{{{\text{catalase, acetylsalicylic acid}}}}{\text{H}}_{{\text{2}}} {\text{O}} + 0.5{\text{O}}_{{\text{2}}} \), was developed for the quantitative determination of acetylsalicylic acid. Calibration graphs of the reciprocal value of first-order rate constant versus acetylsalicylic concentration covered the concentration range (2.99–19.98)×10−4 mol/L, while the detection limit was 4.12×10−4 mol/L acetylsalicylic acid with a standard deviation of 2.1×10−5 mol/L.

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References

  1. Nicholls P, Schonbaum GR (1963) Catalases. In: Boyer PD, Lardy H, Myrback K (eds) The enzymes. Academic Press, Orlando, 8:147–225

  2. Chance B, Sies H, Boveris A (1979) Physiol Rev 59:527–605

    CAS  PubMed  Google Scholar 

  3. Chance B (1947) Acta Chem Scand 1:236–267

    CAS  Google Scholar 

  4. Jones P, Suggett A (1968) Biochem J 110:617–620

    CAS  PubMed  Google Scholar 

  5. George P (1949) Biochem J 44:197–205

    CAS  Google Scholar 

  6. Chance B, Herbert D (1950) Biochem J 46:402–414

    CAS  Google Scholar 

  7. Lardinois OM, Mestdagh MM, Rouxhet PG (1996) Biochim Biophys Acta 1295:222–238

    CAS  PubMed  Google Scholar 

  8. Kremer ML (1981) J Phys Chem 85:835–839

    CAS  Google Scholar 

  9. Chance B (1949) J Biol Chem 179:1299–1309

    CAS  Google Scholar 

  10. Ogura Y, Tonomura Y, Hino S, Tamiya H (1950) J Biochem–Tokyo 37:153–177

    Google Scholar 

  11. Margoliash E, Novogrodsky A (1958) Biochem J 68:468–475

    CAS  PubMed  Google Scholar 

  12. Sultan SM (1987) Analyst 112:1331–1333

    CAS  PubMed  Google Scholar 

  13. Geeta N, Baggi TR (1988) Microchem J 38:232–235

    CAS  Google Scholar 

  14. Glombitza BW, Schmidt PC (1994) J Pharm Sci 83:751–757

    CAS  PubMed  Google Scholar 

  15. Ruiz Medina A, Fernandez de Cordova ML, Ortega-Barrales P, Molina-Diaz A (2001) Int J Pharm 216:95–104

    Article  PubMed  Google Scholar 

  16. Sena MM, Fernandes JCB; Rover L, Poppi RJ, Kubota LT (2000) Anal Chim Acta 409:159–170

    Article  CAS  Google Scholar 

  17. Konstantianos DG, Ioannou PC, Efstathiou CE (1991) Analyst 116:373–378

    CAS  PubMed  Google Scholar 

  18. Santoni G, Fabbri L, Gratteri P, Renzi G, Pinzauti S (1992) Int J Pharm 80:263–266

    Article  CAS  Google Scholar 

  19. Di Pietra AM, Gatti R, Andrisano V, Cavrini V (1996) J Chromatogr A 729:355–361

    Article  PubMed  Google Scholar 

  20. Franeta JT, Agbaba D, Eric S, Pavkov S, Aleksic M, Vladimirov S (2002) Il Farmaco 57:709–713

    Article  CAS  PubMed  Google Scholar 

  21. Garrido JMPJ, Lima JLFC, Matos CD (2000) Collect Czech Chem C 65:954–962

    Article  CAS  Google Scholar 

  22. Rover L, Garcia CAB, De Oliveira Neto G, Kubota LT, Galembeck F (1998) Anal Chim Acta 366:103–109

    Article  CAS  Google Scholar 

  23. Ferreira VJF, Cavalheiro ACV, Fagnani E, De Morales M, Pezza L, Pezza HR, Melios CB (1999) Anal Sci 15:249–253

    CAS  Google Scholar 

  24. Su Y, Tomassetti M, Sammartino MP, Crescenti G, Campanella L (1995) J Pharmaceut Biomed 13:449 – 457

    Article  CAS  Google Scholar 

  25. Pasekova H, Sales MG, Montenegro MC, Araujo AN, Polasek M (2001) J Pharm Biomed Anal 24:1027–1036

    Article  CAS  PubMed  Google Scholar 

  26. Milagres BG, Oliveira Neto G, Kubota LT, Yamanaka H (1997) Anal Chim Acta 347:35–41

    Article  Google Scholar 

  27. Catarino RIL, Garcia MBQ, Lapa RAS, Lima JLFC, Barrado E (2002) J AOAC Int 85:1253–1259

    CAS  PubMed  Google Scholar 

  28. Quintino MSM, Corbo D, Bertotti M, Angnes L (2002) Talanta 58:943–949

    Article  CAS  Google Scholar 

  29. Popov Ch, Popova M, Popova T (1983) Dok Bolg Akad Nauk 36:1343–1346

    CAS  Google Scholar 

  30. Vogel WH, Snyder R, Schulman MP (1964) J Pharmacol Exp Ther 146:66–73

    CAS  PubMed  Google Scholar 

  31. Jones P, Suggett A (1968) Biochem J 108:833–838

    CAS  PubMed  Google Scholar 

  32. Trinder P (1954) Biochem J 57:301–304

    CAS  PubMed  Google Scholar 

  33. Nelson DP, Kiesow LA (1972) Anal Biochem 49:474–478

    CAS  PubMed  Google Scholar 

  34. Jones P, Wynne-Jones WFK (1962) T Faraday Soc 58:1148–1158

    CAS  Google Scholar 

  35. Kremer ML (1983) J Chem Soc Farad T 1 79:2125–2131

    CAS  Google Scholar 

  36. Michaelis L, Menten M (1913) Biochem Z 49:333–369

    CAS  Google Scholar 

  37. Lineweaver H, Burke D (1934) J Am Chem Soc 56:658–666

    CAS  Google Scholar 

  38. Bonnichsen RK, Chance B, Theorell H (1947) Acta Chem Scand 1:685–709

    Google Scholar 

  39. Beers RF, Sizer IW (1952) J Biol Chem 195:133–140

    CAS  Google Scholar 

  40. Gelpi JL, Aviles JJ, Busquets M, Imperial S, Mazo A, Cortes A (1993) J Chem Educ 70:805–816

    CAS  Google Scholar 

  41. Kellner R, Mermet JM, Otto M, Widmer HM (1998) Analytical Chemistry. Wiley-VCH, Weinheim, Germany

Download references

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Authors and Affiliations

  1. Faculty of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 3400, Cluj-Napoca, Romania

    Claudia Mureşanu & Lucian Copolovici

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  1. Claudia Mureşanu
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  2. Lucian Copolovici
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Correspondence to Claudia Mureşanu.

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Mureşanu, C., Copolovici, L. Kinetic method for acetylsalicylic acid determination based on its inhibitory effect upon the catalytic decomposition of H2O2 . Anal Bioanal Chem 378, 1868–1872 (2004). https://doi.org/10.1007/s00216-003-2470-4

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  • DOI: https://doi.org/10.1007/s00216-003-2470-4

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