Determination of ferulic acid and adenosine in Angelicae Radix by micellar electrokinetic chromatography

Abstract

A micellar electrokinetic chromatography for determining ferulic acid and adenosine in Angelicae Radix was developed. A buffer solution composed of 50 mmol L⁻¹ borax, 10 mmol L⁻¹ sodium deoxycholate, and 2% methanol was found to be the most suitable electrolyte for the separation. The contents of ferulic acid and adenosine in Angelicae Radix were determined within 20 min. Good linearity between peak area ratio and the concentration was found in the range of approximately 20–320 μg mL⁻¹ for ferulic acid and about 10–160 μg mL⁻¹ for adenosine (r >0.998), respectively. The recoveries were approximately 96.8–97.4% and 93.2–95.0%, and the RSD of this proposed method were 4.4% and 3.2% for ferulic acid and adenosine, respectively (n=5). The contents of ferulic acid and adenosine in Angelicae Radix from different sources were determined.