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Cathodic adsorptive stripping square-wave voltammetric determination of nifedipine drug in bulk, pharmaceutical formulation and human serum

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Abstract

Nifedipine is a calcium-channel antagonist drug used in the management of angina pectoris and hypertension through inhibition of calcium influx. A fully validated sensitive cathodic adsorptive stripping square-wave voltammetry procedure was optimized for the determination of the drug at trace levels. The procedure was based on the reduction of the nitrophenyl group after the interfacial accumulation of the drug onto a hanging mercury drop electrode in Britton–Robinson buffer of pH 11.0. The optimal conditions of the procedure were found to be: accumulation potential=–0.9 V vs. Ag/AgCl/KCls), accumulation time=30 s, scan increment=10 mV, pulse amplitude=50 mV and frequency=120 Hz. Under these conditions, a well-defined peak was obtained; its peak current showed a linear dependence on drug concentration in the range of 2×10–9–2×10–7 mol L–1 bulk nifedipine. The mean recoveries based on eight replicate measurements for 1×10–8 and 5×10–8 mol L–1 bulk nifedipine solutions were 98.46±0.86% and 98.23±0.92%, respectively. A detection limit of 3.42×10–10 mol L–1 bulk nifedipine was achieved. The procedure was successfully applied for assay of the drug in tablets and spiked human serum with mean recoveries of 101.95±1.42% and 98.70±0.63%, respectively. The limit of detection of the drug in spiked human serum was found to be 3.90×10–10 mol L–1.

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Acknowledgement

The authors express their gratitude to the Alexander von Humboldt Foundation (Bonn, Germany) for donation of the Electrochemical Trace Analyzer Model 263A—PAR, the Eppendorf centrifuge 5417C and the PC-Computer used in the present study.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Tanta University, 31527, Tanta, Egypt

    M. M. Ghoneim & A. Tawfik

  2. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71516, Assiut, Egypt

    P. Y. Khashaba

Authors
  1. M. M. Ghoneim
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  2. A. Tawfik
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  3. P. Y. Khashaba
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Correspondence to M. M. Ghoneim.

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Ghoneim, M.M., Tawfik, A. & Khashaba, P.Y. Cathodic adsorptive stripping square-wave voltammetric determination of nifedipine drug in bulk, pharmaceutical formulation and human serum. Anal Bioanal Chem 375, 369–375 (2003). https://doi.org/10.1007/s00216-002-1703-2

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  • DOI: https://doi.org/10.1007/s00216-002-1703-2

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