Abstract
The simplest proton pump known in biological systems, bacteriorhodopsin (bR), is the first ion-transporting membrane protein, the function of which can be described at the atomic level, with the aid of molecular dynamics calculations. To get additional experimental support for the proposed atomic level description of the function of bR, we studied a quasi-stable state of the protein molecule, the so-called M intermediate that plays a crucial role in the proton pumping process. The temperature dependence of the light-induced events occurring in the photocycle of wild-type bacteriorhodopsin and its mutant D96N were followed in detail. Absorbance changes, electric signals generated by charge motion inside the protein, and movement of protons in the protein solution interface either forward (proton release due to excitation of bR) or backward (uptake of protons due to the M excitation: “back-take”) were monitored. The obtained Arrhenius parameters indicate that the proton back-take is triggered by charge rearrangements in the protein similar to the proton release triggered by those during the L → M transition. The time necessary for proton back-take determines the reconstitution time of the bR ground state. The data are expected to be used in theoretical modeling of the bR function. Based on these results, a more detailed photocycle model is established to describe the proton pumping mechanism, implying a formal principle ("domino model") that is expected to hold also for other charge transfer proteins.
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Acknowledgments
Discussions with Prof. Sándor Suhai on the general aspects of the bacteriorhodopsin proton pump mechanism are gratefully acknowledged. The research was supported by the Hungarian National Science Fund (OTKA T-049489 and CK 78367). S.G.T. is a visiting professor at the University of the Basque Country and an associate member of the Institute of Biophysics Bulgarian Academy of Sciences.
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Dedicated to Professor Sandor Suhai on the occasion of his 65th birthday and published as part of the Suhai Festschrift Issue.
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Tóth-Boconádi, R., Dér, A., Taneva, S.G. et al. Excitation of the M intermediates of wild-type bacteriorhodopsin and mutant D96N: temperature dependence of absorbance, electric responses and proton movements. Theor Chem Acc 125, 365–373 (2010). https://doi.org/10.1007/s00214-009-0632-y
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DOI: https://doi.org/10.1007/s00214-009-0632-y