Abstract
Myrtus communis L. (Family: Myrtaceae) is naturally found in the western part of Asia, Southern Europe, and North Africa. It has been reportedly applied in pharmaceutical industry, traditional medicine, cosmetics, spices, and food. Pubmed, Google scholar, Web of Science, and Scopus were utilized to seek out relevant content concerning the therapeutic potential of M. communis. Subsequently, we conducted a review to identity noteworthy updates pertaining to M. communis. Myrtle berries, leaves, seeds, and essential oils are natural sources of several nutrients and bioactive compounds with marked health effects. The chemical analysis showed that M. communis contained oils, alkaloids, flavonoids, phenolics, coumarins, saponosides, tannins, quinines, and anthraquinones. A pharmacological investigation revealed that M. communis possessed anti-inflammatory, analgesic, antimicrobial, antiparasitic, antioxidant, antidiabetic, anticancer, antimutagenic, immunomodulatory, dermatological, cardiovascular, central nervous system, and gastrointestinal protective effects, among numerous other biological effects. This current review focused on the biochemical, pharmacological, therapeutic effects, and various biological activities of different parts of M. communis. It signifies that M. communis is a therapeutic plant with numerous applications in medicine and could be used as a drug isolate based on its safety and effectiveness.
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Introduction
Myrtle (Myrtus communis L.) is an aromatic medicinal plant, typical of the coastal areas of the Mediterranean regions, such as North Africa or Southern Europe, but it is also present in South America, Australia, and in some areas of Himalaya (Alipour et al. 2014; Jabri et al. 2018; Sumbul et al. 2010, Gaber et al., 2021). It belongs to the Myrtaceae family, which includes about 3000 species, and grows spontaneously as an evergreen shrub or a small tree. The plant can reach a height of 2.5 m, with a full head deeply covered by branches and small leaves; flowers are starry, scented, and can be white or pink, whereas berry fruits are edible, small, with a round shape and many seeds inside, generally blue-black, even if some varieties have white-yellow fruits, and ripen in autumn, between October and February (Fig. 1).
Secondary metabolites are produced by biologically synthesizing the primary metabolites of plants which are the major composition of chemicals in agrochemicals, food additives, biopesticides, colors, fragrances, flavors, and pharmaceuticals (Al-Snafi 2013a, b; Al-Snafi 2014a, b; Al-Snafi 2016; Al-Snafi 2018; Salehi et al. 2019; Gaber et al; 2021; Al-Snafi 2021; Al-Snafi, Ibraheemi & Talab 2021). M. communis has been reportedly applied traditionally in medicine, spices, and food preparation. The chemical analysis showed that M. communis contained oils, alkaloids, flavonoids, phenolics, coumarins, saponosides, tannins, quinines, and anthraquinones. A pharmacological investigation revealed that M. communis possessed anti-inflammatory, analgesic, antimicrobial, antiparasitic, antioxidant, antidiabetic, anticancer, antimutagenic, immunomodulatory, dermatological, cardiovascular, central nervous system, and gastrointestinal protective effects, among numerous other biological effects.
Many plants that have therapeutic properties have recently been reviewed showcasing their biological and pharmacological properties and expanding their prospect in the drug development pipeline against the management of various diseases (Batiha et al., 2023a, b; Teibo et al. 2021).
A comprehensive search was carried out on Pubmed, Google scholar, Web of Science, and Scopus to seek out relevant content concerning the therapeutic potential of M. communis. Subsequently, we conducted a review to identity noteworthy updates pertaining to M. communis which was used for the study.
This current review focused on the biochemical and pharmacological constituents and therapeutic effects of M. communis.
Taxonomic classification
Kingdom | Plantae |
Subkingdom | Viridiplantae |
Infrakingdom | Stretophyta |
Superdivision | Embryophyta |
Division | Tracheophyta |
Subdivision | Spermaophytina |
Class | Magnoliopsida |
Superorder | Rosanae |
Order | Myrtales |
Family | Myrtaceae |
Genus | Myrtus |
Species | Myrtus communis |
Distribution
M. communis has its origins in the western part of Asia, northern Africa, and southern Europe. It was dispersed across Africa (South Africa, Algeria, Ethiopia, Eritrea, Libya, Morocco, Tunisia), Asia (Yemen, Afghanistan, Cyprus, Iraq, Iran, Jordan, Syria, Lebanon, Turkey, Palestine, Pakistan), Europe (Albania, France, Greece, Portugal, Malta, Spain, Former Yugoslavia) (Aslam et al. 2010; Jabri et al. 2016a, b, c).
Description
It is a bushy, strong-scented evergreen, upright shrub, about 3 m high, that arches as the year goes by. The leaves are in threes, simple, and have the shape ovate-lanceolate (Fig. 1). The leaf length is 2.5–5 cm, its color is dark green, and other features include aromatic when crushed; the glossy nature; short petioles; veins that are pinnated and glabrous; the flowers that are white or pink; solitary, axillary; and could be rose-tinged. It has the shape of a bowl, with a length of 2 cm. The plant is also actinomorphic, bisexual, and epigynous with slender pedicels of length 2 cm; the calyx is turbinate calyx tube of four to five sepals; the corolla is four to five petalled; fruit is oblong-ellipsoid white and 1 cm long (Bouzabata 2013). The berry fruits are edible, small, with a round shape and many seeds inside, generally blue-black, even if some varieties have white-yellow fruits, and ripen in autumn, between October and February. Insects do pollination, and birds spread seeds in the environment (Alipour et al. 2014; Petretto et al. 2016).
Traditional uses
All the aerials’ parts, fruits, leaves, and essential oil are medicinal. The applications of myrtle are in food, spices, and traditional medicine. The decoction of myrtle aerial part was used as hypotensive, hypoglycemic, anti-inflammatory, and antidiarrhea in the treatment of bleeding, conjunctivitis, epistaxis, peptic ulcers, palpitation, urethritis, hemorrhoids, headache, leukorrhea, excessive perspiration, skin diseases such as pulmonary treating cough, gastrointestinal disorders (i.e., peptic ulcers, diarrhea, and hemorrhoids), urinary diseases (i.e., urethritis), and skin ailments (i.e., reddened skin), as well as for inactivating microorganisms and wound healing (Sumbul et al. 2010; Boudjelal et al. 2013; Aleksic and Knezevic 2013, Aleksic et al. 2014). Fruits have a wide range of applications: as astringent, analgesic, antiseptic, carminative, demulcent, anti-inflammatory, diuretic, antidiabetic antiemetic, nephroprotective, homeostatic, and for stomach disorders. Leaves were applied as flavoring agent, astringent, antiseptic, blood purifier hypoglycemic, laxative, analgesic, homeostatic, stimulant, and in the treatment of constipation and respiratory diseases (Sumbul et al. 2012; Dellaoui et al. 2018).
Physicochemical characteristic
M. communis berry
Myrtle berries are round fruits composed of a fleshy pericarp and a snail-shaped seed (Pezhmanmehr et al. 2010). Table 1 below shows the physicochemical properties of berries of M. communis.
(Sumbul et al. 2012)
M. communis seed
Since myrtle fruit is rich in secondary metabolites, the seeds in particular have previously been reported to contain a larger number of phenolic compounds in comparison with other tissues in the pericarp (Wannes and Marzouk 2013; Taamalli, et al. 2014b, c, a; Andrea et al. 2019). Ellagic acid is released after hydrolysis of ellagitannins, and its presence in myrtle berries is well documented (Taamalli et al. 2014b, c, a; Taamalli et al. 2014b, c, a). Two main dimeric ellagitannins, eugeniflorin D2 (Al-Snafi 2013a, b) and oenothein B (Al-Snafi 2013a, b), with a specific macrocyclic structure were isolated from seed extracts and chemically characterized (Table 2).
Chemical constituents
Table 3 below shows the analysis of the phytochemical constituent in the fruit of M. communis that contained oils, alkaloids, flavonoids, phenolics, coumarins, saponosides, tannins, quinines, and anthraquinones (Mahboubi and Ghazian 2010; Sumbul et al. 2012; Dellaoui et al. 2018).
Quantitative analysis showed that berries of M. communis contained tannins 2.34 ± 0.07% (Sumbul et al. 2012).
(Sumbul et al. 2012)
Table 4 shows the oil produced by M. communis from Iran is 0.17% from fruit, 1.3% from leaves when ripe, and 2.61% from leaves at flowering stage. During the flowering stage, oil production by M. communis leaves was α-pinene (3.8 - 23.0 %), 1,8-cineole (9.9 - 20.3 %), limonene (5.5 - 17.8 %), linalool (12.3 - 17.6 %) and α-terpinyl acetate (1.8 - 7.0 %). The compositions of the leaf oil at fruit ripening stage was highly similar to those of flowering, the main components: 1,8-cineole (24.0-36.1 %), α-pinene (22.1-22.5%), limonene (3.8-17.6 %), linalool (8.4-11.4 %), linalyl acetate (4.2-4.5%), α-terpinyl acetate (2.2-4.4 %), and geranyl acetate (1.2 %).
From Turkey, four different M. communis fruit essential oils were analyzed chemically and showed oxygenated monoterpenes (73.02–83.83%) were found in excess. The major oil constituents were α-pinene (6.04–20.71%), α-terpineol (8.40–18.43%), 1,8-cineole (29.20–31.40%), geranyl acetate (3.98–7.54%), and linalool (15.67–19.13%) (Kordali et al. 2016).
From Northern Iraq, M. communis fruits components were dodecane (11.39%), oleic acid methyl ester (21.18%), linoleic acid methyl ester (27.19%), octane3,5-dimethyl (16.47%), stearic acid methyl ester (3.32%) tetradecane (6.69%), and palmitic acid methyl ester (6.80%) (Qader et al. 2017).
From the northwestern Tunisia in Bni Mtir, Wild M. communis aerial parts essential oil showed α-terpineol (9.45 to 9.72%), 1,8-cineole (42.58 to 51.39%), linalool (5.91 to 6.06%), and methyl eugenol (6.69 to 7.11%) were the main constituents (Messaoud et al. 2012).
Analysis of essential oils of myrtle leaf from Algeria through the use of GC-MS and GC showed oils having 51 constituents rich in oxygenated monoterpenes 37.47–55.23%, monoterpene hydrocarbons 36.30–51.36%, sesquiterpene hydrocarbons 3.04–4.74%, and oxygenated sesquiterpenes 1.89–2.86%. Their major compounds were α-pinene (30.65–44.62%) and 1,8-cineole (25.46–32.12%) (Berka-Zougali et al. 2012).
GC-MS analysis of M. communis oils from the Benslimane region of Morocco showed α-terpineol (15.5%), geranyl acetate (11.64%), and methyl eugenol (18.7%) in excess quantity. In comparison, Ouazzane-Morocco essential oil has abundant, rich 1,8-cineole (36.3%) (Harassi et al. 2019).
From Italy, M. communis essential oil contained α-pinene (41.6–28.9%), α-terpineol (3.6%), 1,8-cineole (25.5–24.2%), trans-myrtanol acetate (4.2–5.2%), limonene (9.5–5.2%), and linalool (11.7%), as well as the main extract (Flaminia et al. 2004).
M. communis var. italica leaf and flower essential oil composition was α-pinene (17.53% for flower and 58.05% for leaf) in excess. 1,8-cineole (32.84%) was seen in the stem extract due to excess oxygenated monoterpenes (Aidi et al. 2010).
From Aventis in France, M. communis essential oil analysis showed 1,8-cineole (19.6%), linalool (12.6%), and α-pinene (24.7%) (Mahmoudvand et al. 2015), though two samples of chemical profiling from northern and southern Montenegro beaches showed that monoterpenes were the main constituents (linalool, myrtenyl acetate, α-pinene, and 1,8-cineole). The samples had myrtenyl acetate (5.4–21.6%) and α-pinene (14.7–35.9%) (Mimica-Dukic et al. 2010).
M. communis leaves isolate includes phloroglucinols (myrtucommulones A-L), arjun olic acid, asiatic acid, 23-dihydroxyolean-12-en-28-oic acid, 3βcis-p-coumaroyloxy-2α, 23-hydroxyursolic acid, hederagenin, 3β-O-cis-p-coumaroyl-2αhydroxy-urs-12-en-28-oic acid, 3β-O-trans-p-coumaroyl maslinic acid, 3β-trans-p-coumaroyloxy-2α,24-dihydroxy-urs-12-en-28-oic acid, maslinic acid, coprozoic acid, jacoumaric acid, oleanolic acid, botulinic acid, ursolic acid (Rotstein et al. 1974; Taamalli et al. 2014b, c, a; Appendino et al. 2006; Khan et al. 2019; Liang et al. 2019; Franco et al. 2019).
Phenolics (ellagic acid, caffeic acid, ferulic acid, quinic acid, gallic acid, (−) epicatechin-3-O-gallate, (−) epigallocatechin-3-O-gallate, catechin, (−) epigallocatechin), and flavonoids (kaempferol, quercetin-3-O-glucoside, quercetin, quercetin-3-rutinoside, quercetin-3-O-rahmnoside myricetin, myricetin-3-O-galactoside, myricetins 3-O-alpha-L-rhamnoside, myricetins 3-O-beta-D-xyloside, myricetin 3-O-beta-D-galactoside, myricetin-3-O-arabinoside, myricetin-3-O-rahmnoside, hesperidin, esculin, patuletin) were M. communis alcoholic extract of the fruits and leaf. Hydrolysable b tannins (eugeniflorin D, eugeniflorin D2, oenothein B, tellimagrandins I, including tellimagrandins II) and condensed tannins, petunidin-3-O-glucoside, delphinidin-3-O-glucoside, malvidin-3-O-glucoside, cyanidin-3-O-glucoside, peonidin-3-O-glucoside, delphinidin-3-Oarabinoside, petunidin-3-O-arabinoside, and also malvidin-3-O-arabinoside, were identified in M. communis ( Aidi et al. 2010; Romani et al. 1999; Montoro et al. 2006; Yoshimura et al. 2008; Barboni et al. 2010; Tuberoso et al. 2010; Bouaziz et al. 2015; Bouaoudia-Madi et al. 2017).
The flavonoid contents and total phenol were abundant in the leaf extract (13.65 mg GAE/g dry weight as well as 250 mg GAE/g dry weight, respectively), and anthocyanin and tannin contents (176.50 ± 2.17 mg Cyd-3-glu /g dry weight and 220.81±1.21 mg CE /g dry weight), respectively, were abundant in the extract of the pericarp (Bouzabata et al. 2015).
The total phenolics and flavonoids were assessed in aqueous extracts, ethyl acetate, chloroform, methanol of M. communis leaves. Elevated total phenolic and total flavonoid contents (435.37 mg gallic acid equivalents/g dried weight also 130.75 mg quercetin equivalent/g dried weight, respectively) were seen in the ethyl acetate extract (Hosseinzadeh et al. 2011).
Different myrtle showed diversity in the total phenol contents (M. communis var. italica) parts; flower (15.70 mg GAE/g), stem (11.11 mg GAE/g), and leaf extract (33.67 mg GAE/g) extracts. The total tannin contents of myrtle different parts is as follows: (3.33 mg GAE/g in stem, 11.95 mg GAE/g in the flower, and 26.55 mg GAE/g in leaf). The stem extract showed the main contents of condensed tannins, total flavonoids, (5.17 and 1.99 mg CE/g individually), and leaf extract (3 and 1.22 mg CE/g, respectively). The HPLC analysis showed hydrolysable tannins (gallotannins) in leaf (79.39%, 8.90 mg/g) and flower (60.00%, 3.50 mg/g) are the predominant class of phenolics, whereas the flavonoid class is the most abundant (61.38%, 1.86 mg/g) due to excess catechin (36.91%, 1.12 mg/g) (Mimica-Dukic et al. 2010).
The phenolic compounds content in myrtle aerial parts infusions with different times of preparation ( 5, 10 and 15 min), respectively, was (μ mol/g dry matter): gallic acid 6.47 ± 0.59, 8.23 ± 1.23, and 11.82 ± 0.65; caffeic acid 0.71 ± 0.18, 0.88 ± 0.29, and 1.41 ± 0.06; syringic acid 0.18 ± 0.06, 0.29 ± 0.24, and 0.53 ± 0.12; ferulic acid 0.29 ± 0.08, 0.41 ± 0.06, and 0.53 ± 0.13; myricetin-3-ogalactoside 0.59 ± 0.09, 0.76 ± 0.08, and 1.06 ± 0.15; myricetin-3-orhamnoside 0.71 ± 0.11, 0.82 ± 0.10, and 1.18 ± 0.16; myricetine-3-oarabinoside 0.12 ± 0.09, 0.18 ± 0.07, and 0.24 ± 0.04; quercetin-3-O-galactoside 5.35 ± 0.18, 6.64 ± 1.29, and 9.11 ± 1.00; quercetin-3-O-rhamnoside 0.29 ± 0.18, 0.29 ± 0.12, and 0.53 ± 0.17; myricetin 3.00 ± 0.24, 3.82 ± 0.29, and 5.00 ± 0.65; quercetin 1.59 ± 0.47, 2.41 ± 0.35, and 3.59 ± 0.59; total identified phenolic compounds 19.28 ± 2.12, 24.75 ± 4.06, and 34.98 ± 3.47; phenolic acids 7.64 ± 0.88, 9.82 ± 1.82, and 14.28 ± 0.88; flavonol glycosides 7.05 ± 0.53, 8.70 ± 1.59, and 12.11 ± 1.35; and flavonols 4.58 ± 0.71, 6.23 ± 0.65, and 8.58 ± 1.23 (Harassi et al. 2019).
Mineral contents of the leaves of M. communis were: P 547± 28, Na 133 ±19, Zn 25 ±1, Si 2 ±3, Cu 9.1 ±1, Ni 0.5 ±0.1, Cr 2±0.4, Pb 0.5 ±0.5, Mo 0.5 ±0.1, V 0.27 ± 0.1, and Cd 0.24 ±0.1 µg/g dry weight (Messaoud et al. 2012).
Anti-inflammatory
M. communis essential oil was assessed for anti-inflammatory property using lipopolysaccharide (LPS)-stimulated macrophages in vitro model; oils significantly inhibited the production of NO without altering the viability of the cell at 0.64 mg/ml concentrations (Cruciani et al. 2019). The aqueous extracts (0.03, 0.015, 0.005 g/kg) and ethanolic (0.05 g/kg) possessed anti-inflammatory action against prolonged inflammation (Soomro et al. 2019). M. communis essential oil anti-inflammatory activity was assessed by mice with ear edema induced by croton oil and myeloperoxidase activity, and in rats’ models, granuloma induced by cotton pellet and serum TNF-α as well as IL-6 (Feisst et al. 2005). The topical administration of the essential oil reduced edema in the ear, cotton pellet-induced granuloma, MPO activity, serum IL-6, and TNF-α. The bicyclic monoterpenoid myrtenal, which was isolated from M. communis essential oil (5%), was tested for anti-inflammatory effects in rodent models.
The anti-inflammatory action of oenothein B isolated from M. communis seeds was assayed in vitro. It possessed anti-inflammatory properties. M. communis pulp and seed extracts were evaluated on human fibroblast for their effects against inflammation. The production of ROS was determined after H2O2 treatment induced oxidative stress and expressions of gene of various proinflammatory cytokines and CYP3A4 and CYP27B1. The results revealed the synergic effect of Myrtus extracts with vitamin D to reduce inflammation, ROS production, thereby shielding cells from damages caused by oxidative stress, modulated CYP expression, and preventing chronic inflammation (Dragomanova et al. 2019).
M. communis leaf isolate oligomeric non-prenylated acylphloroglucinols (semi myrtucommulone and myrtucommulone A) directly inhibited 5-lipoxygenase and COX-1 in vivo, including in vitro, with IC50 value around 1.8 to 29 microM, thereby significantly reducing eicosanoids biosynthesis. In polymorphonuclear leukocytes, calcium mobilization is prevented by the administration of myrtucommulone and semi myrtucommulone, which suppresses the concentration ROS and elastase formation, which is mediated through the signaling pathway of G protein of IC50 values around 0.55 and 4.5 microM, respectively (Alem et al. 2008).
M. communis extract myrtucommuacetalone-1 (MCA-1) was assessed for anti-inflammation using hydrogen peroxide, superoxide, and macrophages nitric oxide assays. The translocation and phosphorylation transcription factor NF kappa B, iNOS activation regulator, was used to analyze the compound. MCA-1 action on the iNOS enzyme was also tested. In excited macrophages, MCA-1 caused the inhibition of hydrogen peroxide, superoxide, with 53% and 48% respectively, through nitric oxide an IC50 of <1 µg/ml, showing solid binding pattern at the enzyme nitric oxide synthase active site. In addition, MCA-1 of 25 µg/ml stopped the expression of inducible nitric oxide synthase and eliminated the translocation and phosphorylation of transcription factor (NFκB) into the nucleus (Maxia et al. 2011).
M. communis extract acylphloroglucinol myrtucommulone effect on PGE2 synthase mPGES-1 and subsequent inhibition of prostaglandins as an intermediary of pain and inflammation was examined using the A549 cells stimulated by interleukin-1beta- prepared in a microsomal means for a precursor for mPGES-1, lipopolysaccharide-stimulated human whole blood, and intact cells A549 in a cell-free assay. Production of 6-oxo PGF 1α and 12(S)-hydroxy-5-cis-8,10-trans-heptadecatrienoic acid confirmed that the activity of COX-1 and COX-2 was inhibited in the cell-free and cellular tests. The conversion of PGE2 from PGH2 was done by cell-free mPGES-1-mediated (IC50 = 1 micromole/l). Myrtucommulone concentration-dependently inhibited the production of PGE2, which decreased significantly in whole human blood and complete A549 cells at small micromolar concentrations. Activities of COX-2 in A549 cells and isolated human recombinant COX-2 were significantly inhibited, but COX-1 was inhibited slightly in cell-free and cells by the action of myrtucommulone (30 micromole/l) and (IC50> 15 micromole/l) respectively (Rossi et al. 2009).
Using a mouse model, inflammation was brought about by the intrapleural administration of carrageenan, while intraperitoneal of treatment myrtucommulone was given (0.5, 1.5, and 4.5 mg/kg). The result obtained showed the suppression of proinflammatory cellular response. The administration of 4.5 mg/kg myrtucommulone ip at half of an hour before and after the introduction of carrageenan causes the decrease in the amount of leukocyte in the blood, neutrophil infiltration seen through myeloperoxidase, lung injury, lung intercellular adhesion molecule-1 including P-selectin immunohistochemical localization, an equally pleural fluid reduction in the cytokine levels (TNF-α and IL-1β) of the lungs, leukotriene B4. In the pleural fluid, and their immunohistochemical localization in the lung, the leukotriene B4, but not prostaglandin E2, levels in the pleural exudates and lung peroxidation (thiobarbituric acid-reactant substance) and nitrotyrosine and poly (ADP-ribose) immunostaining. Myrtucommulone reportedly suppressed eicosanoids biosynthesis by inhibiting cyclooxygenase-1 and 5-lipoxygenase in vitro, ROS, and elastase formation in activated polymorphonuclear leukocytes (Raoof et al. 2019).
Analgesic effects
M. communis aerial parts of both aqueous and ethanolic extracts were tested for antinociceptive activity by testing with a hot plate and writhing. Antinociception was shown by the aqueous and ethanolic extracts, with marked antinociception inhibited through naloxone. Antinociception was exhibited by extracts against the writhing induced by acetic acid.
The bicyclic monoterpenoid myrtenal, which was isolated from M. communis essential oil (5%), was tested for analgesic. Anti-nociceptive activity (30 mg/kg, bw, ip) was tried on two experimental pain models of male mice using a writhing test of acetic acid including the hot plate, after single administration in addition to repetitive administration.
The bicyclic monoterpenoid myrtenal showed significant antinociception (concerning thermal and peripheral pain). During severe administration, the writhing number of the abdomen significantly decreased in the 15th and 20th minutes (at P< 0.01 and P< 0.05) by 47.25% and 50.55% respectively. It reduced (P<0.001) treatment group number versus control group after continuous treatment, 40.4% on 7th and 14th day 43.1% when assessed with the controls (Koeberle et al. 2009).
Antimicrobial effects
M. communis seed isolate oenothein B was tested for its fungicidal effect on Candida in vitro, and its MIC anticandidal effect was <8–64 μg/ml (Barboni e al. 2010).
The crude extract of myrtle was examined for antimicrobial action against Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Klebsiella aerogenes, Proteus vulgaris, and Proteus mirabilis. The MIC of the extract was (0.5, 2.5, 15, and 20 mg/ml) against S. aureus, P. mirabilis, P. vulgaris, Klebsiella, S. typhi, and P. aeruginosa, respectively. However, there was 18-folds increase in the antibacterial activity after autoclaving at 121 °C for 15 min (Feuillolay et al. 2016).
M. communis aqueous extract possessed antibacterial properties against Escherichia coli (zone of inhibition 11.48–13.04 mm at 50 mg/ml), Bacillus subtilis (zone of inhibition 10.80–13.72 mm at 50 mg/ml), and Pseudomonas aeruginosa (zone of inhibition 14.06–18.76 mm at 50 mg/ml), but did not show fungicidal against Aspergillus oryzae (Teimoory et al. 2013).
M. communis leaf ethanolic extract was examined for antibacterial effect (0, 15, 30, 45, 60 mg/ml) against Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus and Gram-negative bacteria (Escherichia coli and Klebsiella pneumonia). The results revealed the potency of extracts against all the bacteria tested. Escherichia coli showed the highest sensitivity to the extract of M. communis. The extract was applied in the study of its action on biofilm formation. High sensitivity was seen when biofilm formation was assessed on all the bacterial strains with Escherichia coli and Bacillus subtilis being the most potent to M. communis extract (Sidkey 2006; Rasaie et al. 2017). An acylphloroglucinols (myrtucommulone-A) isolated from M. communis leaves exhibited great antibacterial activity versus Gram-positive bacteria, though inactive on the Gram negatives (Appendino et al. 2006).
The antimicrobial effect of M. communis extracts was studied against E. coli, E. coli ATCC 25922, P. mirabilis, S. typhi, Shigella flexneri, and K. pneumonia. All M. communis inoculates exhibited various degree of inhibition antimicrobial activity against all strains tested. The microbial extracts and strains showed inhibition zones values ranging from 21 to 25 mm. According to microbial strains and infusions. The values of MBC and MIC showed ranges from 12.5 to 25 mg dry leaves/ml and 12.5 to 50 mg dry leaves/ml against different isolates, respectively (Harassi et al. 2019).
M. communis alcoholic extract was assessed for its bactericidal effect against Listeria monocytogenes, Bacillus cereus (NCTC 7464 and ATCC 10876), and Staphylococcus aureus (ATCC 25923). At a concentration of 10 mg, M. communis demonstrated a growth inhibitory effect on the three examined bacteria. A maximum effect was observed on Staphylococcus aureus and a minimum effect against Bacillus cereus (Rahimvand et al. 2018). M. communis leaf extracts of water extracts, methanol, n-hexane, ethanol, and ethyl acetate were examined for its antimicrobial activities against Candida albicans ATCC 10239, Escherichia coli ATCC 25922, Escherichia coli ATCC 29998, Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Enterococcus faecalis ATCC 29212, Enterobacter cloacae ATCC 13047, Pseudomonas aeruginosa ATCC 27853, and Salmonella typhimurium CCM 5445. The growth of Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis was stopped by all the extracts. The methanolic extract was potent against the growth of Escherichia coli. All the extracts did not show any activity against Enterococcus faecalis ATCC 29212, Candida albicans, or Enterobacter cloacae ATCC 13047 (Fani et al. 2014).
M. communis methanolic and aqueous extract antibacterial effect was examined against Prevotella intermedia, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans. M. communis aqueous extract with a range 20 to 500 mg/ml and methanolic extract with a range 10–500 mg/ml possessed antibacterial effects against Prevotella intermedia, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans. M. communis aqueous as well as methanolic extracts showed potency against microorganisms examined with a MIC of 10 mg/ml (Ben et al. 2014).
Ethyl acetate, total methanolic extracts, and extracts of M. communis exhibited good fungicidal action against Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes (Javadi et al. 2017); also, the essential oil acted against Epidermophyton floccosum, Cryptococcus neoformans, Trichophyton rubrum, and Microsporum canis was assessed, respectively (Cruciani et al. 2019). M. communis essential oils exhibited exceptional antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Candida albicans. M. communis oils exposure showed the D-values (decimal reduction value) of (12.8, 2.8, and 8.6 min) S. aureus, E. coli, and C. albicans, respectively (Yadegarinia et al. 2006; Beni et al. 2017).
Myrtle leaf essential oil was extracted using conventional hydro distillation including solvent-free-microwave and the antimicrobial activity against Gram-positive bacteria (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus) Gram-negative bacteria (Salmonella enteric, Escherichia coli, Enterobacter cloacae, Klebsiella pneumonia, Pseudomonas aeruginosa), and fungi and yeast (Aspergillus flavus, Candida albicans, Aspergillus ochraceus, and Fusarium culmorum). Both essential oils exhibited high microbicidal action against both Gram-positive and Gram-negative bacteria (MIC 10–30 µl/ml), yeast, and fungi (MIC 10–50 µl/ml) (Flaminia et al. 2004).
M. communis essential oil microbicidal effect was examined against Streptococcus mutans, Streptococcus sanguinis, and Streptococcus salivarius. According to the results, myrtle leaf essential oil possessed microbicidal activity against every strain of Streptococcus; however, S. mutants has the most of all the species (Cannas et al. 2013).
M. communis oil antimycotic activity was assessed against Candida spp. from clinical isolates. The MIC ranges obtained with the essential oil of myrtle after 24 h were 0.5–2 μg/ml, 0.25–1 μg/ml, 1–4 μg/ml, 0.5–4 μg/ml, 0.5–2 μg/ml for Candida parapsilosis, Candida tropicalis, Candida krusei, Candida glabrata, and C. albicans, respectively. The ranges of MIC after 48 h include 0.5–4 μg/ml, 0.5–2 μg/ml, 2–4 μg/ml, 1–4 μg/ml, 1–2 μg/ml for C. parapsilosis, C. tropicalis, C. krusei, C. glabrata, and C. albicans, respectively (Mert et al. 2008).
The M. communis essential oil antimicrobial activities (3.9–1000 µg/ml) were studied against some oral pathogens (30 strains of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Streptococcus mutans and 20 strains of Candida albicans and Streptococcus pyogenes) gotten out of pharyngitis, periodontal diseases, dental caries, and oral lesions related to artificial dentures. All the extracts had 8.1– 41.25-mm inhibition zones, with the sensitivity to oil at 125–1000 µg/ml. At 62.5 µg/ml, the strain sensitivity of C. albicans, S. mutans, and S. pyogenes was recorded, but only 70% and 66.6% of A. actinomycetemcomitans and P. gingivalis showed resistance to this concentration. At 31.25 µg/ml, the sensitivity of all the strains, S. mutans, and S. pyogenes was recorded. The oil at 7.8 and 15.6 µg/ml sensitivity of S. pyogenes strain was recorded. At 62.5 ± 0, 62.5 ± 0, 46.9 ± 16, 31.25 ± 0, 29.68 ± 4.8 µg/ml, the minimum inhibitory concentrations were recorded against the oil for these microorganisms: P. gingivalis, A. actinomycetemcomitans, C. albicans, S. mutans, and S. pyogenes, respectively (Aleksic et al. 2014).
Some microorganisms were able to access microbicidal activity of essential oils. The ranges for MIC and the zones of inhibition and of the bacteria strains are 0.078–2.5 mg/ml and 16–28 mm, respectively. Gram-negative bacteria showed lower oil activity of inhibition than the gram-positive ones. The oil inhibited several fungal strains significantly. The oil concentration at 312 μg/ml showed bactericidal action against Listeria monocytogenes after 5 min (Fadil et al. 2018).
Three essential oils of M. communis were examined for their action against Acinetobacter baumannii extracted from wound known to be multi-drug. Mytre oils exhibited excellent bactericidal effects, as shown by the result. The drug ciprofloxacin or polymyxin B, when combined with essential oil sun inhibition concentration, decreased the growth of bacterial interdependently (Masoudi et al. 2017).
A synergistic effect was noticed against the strain of Salmonella typhimurium by combining Thymus vulgaris and M. communis essential oils. The preparations include 45% of M. communis and 55% of Thymus vulgaris essential oil, which improved the sensitivity of Salmonella typhimurium (Cannas et al. 2014).
For bacterial vaginosis treatment, M. communis vaginal gel was used alongside with metronidazole gel for 7 days. It was discovered that the administration showed higher potency (P<0.05) compared to their use when not in combination. After 21 days, the patients that were treated with only metronidazole gel had bacterial vaginosis reoccurring, but others administered with a combination of M. communis in metronidazole gel did not show any relapse of the disease (Barac et al. 2018).
The essential oil of M. communis was examined for the formation of biofilm examined in three Candida species (Candida tropicalis, Candida albicans, and Candida parapsilosis). The results showed enhanced action against C. parapsilosis and C. albicans as compared to C. tropicalis. Therefore, anti-biofilm formation is one of its activities (Mehrabani et al. 2013).
The essential oil of M. communis was examined by isolating Malassezia sp. In pityriasis versicolor infection, fungicidal and inhibitory action was seen against Malassezia growth (Mahdi et al. 2006).
Its fungicidal action was examined using M. communis essential oil on various Aspergillus sp. (six isolates of Aspergillus flavus, Aspergillus parasiticus, and Aspergillus niger) and Candida albicans (one ATCC type strains and eight clinical isolates), which showed good inhibitory action against these strains. Also, the combination of amphotericin and essential oil showed an excellent synergistic effect (Penauelas et al. 2001).
Antiparasitic effect
At pH 4.65, M. communis extract killed Trichomonas vaginalis, but at pH 6.00, it was still alive (Chegeni et al. 2019). Both cell culture and cell-free medium were used in anti-Toxoplasma action on M. communis extracts in vitro against Toxoplasma gondii RH strain tachyzoites. The extract caused the inhibition of tachyzoites in cell-free medium. However, the cell culture of M. communis extract and EC50 sensitivity were significantly decreased compared to pyrimethamine (Amiri et al. 2019). At 6.47, 7.62, and 11.64 mg/ml, the LC50 was recorded at 30, 20, and 10 min, respectively (Tayoub et al. 2012). Apoptosis of protocolizes of hydatid cyst was seen when M. communis extract at (50 and 100 mg/ml) was administered through the increased action of caspases 3 and 9 (Amensour et al. 2009).
The essential of M. communis leaf extract possesses fumigant toxicity against Trogoderma granarium at different stages. At 24 and 48 h of exposure to 562.5 µl/l air, the larva showed 94% and 100% death, but the adult was very sensitive at little exposure. The LC90 and LC50 include 487 and 221 µl/l air after 48-h exposure, respectively (Dairi et al. 2014).
In vitro analysis of M. communis extract in water and ethanol (10, 5, 2.5, and 1.25 mg/ml) was conducted for the anti-Acanthamoeba effect of crude for (1–3 day) cysts of Acanthamoeba and on trophozoites. After administration, M. communis ethanolic extract showed 0% for trophozoites viability and 8.62% for cysts.
After 3 days, 0% trophozoites and 31.10% cysts were seen after administering M. communis aqueous extract at 10 mg/ml (Shahnazi et al. 2017). M. communis methanolic and essential oil extracts had a leishmanialicidal effect on the amastigote and promastigote types of Leishmania tropica. The essential oil of M. communis brought about apoptosis to the J774 cells (P<0.05), amastigote and promastigote types of L. tropica in a manner that is dependent on dose. The amastigote as well as promastigote forms showed 11.6 and 40.8 μg/ml and 8.4 and 28.9 μg/ml as their IC50 values after the essential oil and methanolic extract administration. The extract of methanol and essential oils was not cytotoxic to J774 cells. It was seen that M. communis essential oil was more cytotoxic than the methanolic extract (Rotsein et al. 1974). M. communis methanolic extract administered against hydatid cyst protoscoleces showed scolicidal effect at 50 and 100 mg/ml concentrations.
Antioxidant effect
The assays of reducing antioxidant power, and beta-carotene linoleic acid were used to examine action against oxidants, leaf total phenolic content, and M. communis berries. The ranges of the total phenol content include 9.0 mg and 35.6 mg GAE/g of myrtle extract. The berry extracts possess fewer total phenolic compounds, but the leaves have more total phenolic compounds. The aqueous, ethanol, methanol of both leaves and berry extract showed antioxidant activity, but these activities were higher in leaves. The order of these activities includes berry extract (methanol > ethanol > water) and leaf extract (methanol > water > ethanol), respectively (Tumen et al. 2012). The antioxidant activities of phenolic compounds extracted by conventional hydro distillation and microwave-assisted extraction were investigated using different models. The extracts produced by the two extraction techniques produced phenolic compound content that were alike. According to ABTS assay, the extract of myrtle was the highest scavenger compared to α-tocopherol and butylated hydroxyanisole. In ORAC assay, butylated hydroxytoluene was not as strong as both extracts, but the strongest were as myricitrin (myricetin 3-O-rhamnoside) and caffeic acid. The manufacture of conjugated diene andCu2+-induced LDL oxidation during the lag phase responded in a manner dependent on the dose, as myrtle extract and myricitrin inhibited them all. Micelles appearance and bilayer vesicles as seen in the dispersion phospholipid analysis of bile salts through cryo-electron microscopy in bile salts oxidation or 2,2′-azobis (2-amidinopropane) hydrochloride-induced phospholipid myricitrin exhibited a greater protective effect than myrtle extracts, despite the similar effects of caffeic acid with tocopherol and myrtle extract (Ines et al. 2012). M. communis leaf extract 5-O-di-galloylquinic acid (DGQA), when administered on K562 cell line, inhibited lipid peroxidation induced by H2O2, pointing out its antioxidant activity. Also, 82.2% of malondialdehyde formed was inhibited by the pure sample, which equally inhibited H2O2-induced genotoxicity. DGQA increased DNA repair enzymes and antioxidant enzymes; it also prevented gene expression in H2O2 stressed chronic myelogenous leukemia cell line (K562) (Wannes and Marzouk 2016). M. communis extracts showed high antioxidant activity due to derivatives of gallonyl and polyphenols present.
The composition of the hydroalcoholic extracts is ellagitannins, flavanol glycosides, galloyl-quinic acids, galloyl-glucosides, aqueous residues, and ethyl acetate extracts, which have excess flavanol glycosides and hydrolysable tannins (galloyl-quinic acids, ellagitannins, galloyl-glucosides) (Rosa et al. 2003).
The unique oligomeric, nonprenylated acylphloroglucinols (semi myrtucommulone and myrtucommulone A) are M. communis leaf isolate with great antioxidant properties, protected linoleic acid action against free radical attack, FeCl3− and autoxidation inhibition, including EDTA-mediated oxidation. Although the two compounds lack pro-oxidant action, semi myrtucommulone is more potent than myrtucommulone A. It was seen more in hepatic cells homogenates for action against ferric-nitrilotriacetate-induced lipid peroxidation than in cytotoxicity cell cultures for TBH inhibition or oxidation of induced FeCl3. The overall effects confirmed that semi myrtucommulone was a novel dietary antioxidant lead (Demir et al. 2016).
The exceptional oligomeric non-prenylated acylphloroglucinols (myrtucommulone A and semi myrtucommulone) displayed great antioxidant properties in solvent-free and cholesterol thermal (140 °C) degradation. There was significant preservation of LDL from oxidative damage of induced Cu2+ ions after 120 min of oxidation due to the semi myrtucommulone and myrtucommulone. In pre-treatment, oxidative products (7-ketocholesterol, 7-beta-hydroxycholesterol, and conjugated dienes fatty acids hydroperoxides) were inhibited, pointing out great protection because of reduced cholesterol and polyunsaturated fatty acids (Elfellah et al. 1984).
The antioxidant effect of the methanolic extract of the seeds and oil was assessed using 1,1-diphenyl-2-picrylhydrazyl radical scavenging, reducing power β-carotene-linoleic acid bleaching, and metal chelating activity assays. In all tests of myrtle seed methanolic extract, the methanolic extract of myrtle seed exhibited enhanced antioxidant action to the oil (Rosa et al. 2008).
The essential oil and methanolic extract of M. communis var. italica variety were examined for their antioxidant activity using assays like beta-carotene-linoleic acid bleaching, metal chelating activity, and reducing power assays. The methanolic extracts of various parts of myrtle in all the tests displayed more antioxidant action compared to the essential oils (Mimica-Dukic et al. 2010; Cottiglia et al. 2012).
Antioxidant activity was exhibited by the essential oil of Myrtle leaf extracted by solvent-free-microwave extraction and conventional hydro distillation; however, their antioxidant effects were significantly lesser than the quercetin and gallic acid extract. The essential oil of fresh myrtle leaf obtained by solvent-free-microwave extraction showed more antioxidant activity than the one found in conventional hydro distillation (Flaminia et al. 2004).
Antidiabetic effect
M. communis berry aqueous extract was assessed for its hepatoprotective and antidiabetic effects (oral administration for 14 days with 250, 500, or 1000 mg/kg) in diabetic rats induced with streptozotocin. The extract administration significantly reduced AST, ALT, ALP, and serum glucose levels in all the diabetic groups. For the antioxidant, level of activity of SOD and GSH significantly increased, while a reduction of MDA was noticed in the diabetic rats as compared to control (P<0.05). The antioxidant and hypoglycemic effect was seen at a dose of 1000 mg/kg (Baz et al. 2016). M. communis aqueous with an ethanolic extract of (2 g/kg) when intragastrically administered 30 min prior to the injection of streptozotocin eliminated the early hyperglycemia. M. communis extract given 24 h and 30 h prior to streptozotocin inhibited the development of hyperglycemia 48 h later. Hyperglycemia was reduced significantly after administering Myrtus extract 48 h after streptozotocin; this effect was seen after administration. In normal mice, the blood glucose level was unaltered upon Myrtus extract administration (Panjeshahin et al. 2016).
M. communis leaf aqueous extract was investigated for antidiabetic and antioxidant effects in diabetic rats induced with streptozotocin (STZ) and normal rats. MC leaf aqueous extract given at 150, 300, and 600 mg/kg significantly reduced ALT, AST, ALP in serum blood glucose and MDA levels, in STZ-induced diabetic rats when compared to controls groups (P<0.05) (Karimlar et al. 2019).
The leaf hydroalcoholic extract displayed a mild antidiabetic effect in streptozotocin induced diabetic rats. However, the ethanolic extract of leaves (2 g/kg) possessed superior hypoglycemic effect in the diabetic rats than the aqueous extract (P < 0.05) (Tas et al. 2018). The hypolipidemic and hypoglycemic effects of M. communis hydroalcoholic fruits extract were studied in diabetic rats induced with streptozotocin. M. communis hydroalcoholic fruits extract was supplemented with drinking water for 35 days. M. communis hydroalcoholic fruit extract group exhibited reduced lipid profile, serum glucose, and level of malondialdehyde in tissue but increased erythrocyte SOD, aryl esterase, total blood GSH-Px, insulin, and serum paraoxonase activities (Medhat et al. 2017).
Studies were made on lipid profile and blood glucose in diabetic rats induced with streptozotocin-treated M. communis essential oil (200 mg/kg/day). Results pointed out its ability to inhibit α-glucosidase activity in vitro. However, triglyceride (TG), total cholesterol (TC), serum glucose, and low-density lipoprotein cholesterol (LDL) were significantly elevated in diabetic rats than control group (P<0.001). For the normal and diabetic control group, high-density lipoprotein cholesterol (HDL) did not show significance. M. communis oil causes a significant decrease in TC (107±11 and 83±13, P<0.01), TG (167±13 and 118±13, P<0.001), LDL (70±8 and 47±4, P<0.001), glucose (478±24 and 355±48, P<0.001), and increased HDL (37±5 and 53±9, P<0.01). The oil also significantly inhibited α-glucosidase activity (Al-Jeboory et al. 1985).
M. communis possessed α-glucosidase inhibitory activity and protein tyrosine phosphatase 1B (PTP1B), with IC50 values ranges from 34.3 to 88.5 μM and 8.9 to 69.4 μM, respectively. The results supported M. communis application as a bifunctional food for type-2-diabetes treatment or management (Taamalli et al. 2014b, c, a).
Cardiovascular effects
M. communis aqueous extract showed an adverse inotropic effect on isolated Guinea pig atria, which atropine administration did not reverse. The whole extract induced cardiac depressive concentration-dependent effect in rabbits under an anaesthesia (Ebrahimi et al. 2020).
The hypotensive effects of M. communis methanolic and ethyl acetate leaf extracts were assessed in rats under an aesthesia by using invasive blood pressure recording. Intravenous administration of methanol and ethyl acetate reduced the maximum mean arterial systolic and diastolic blood pressure by 32.49% and 20.6% at 12 mg/kg bw, respectively, in rats anesthetized (Hosseinzadeh et al. 2011).
Studies were done to assess the leaf extract of M. communis ability to mitigate endothelial dysfunction and atherosclerosis in ovariectomized rats to model post menopause. Parameters assessed were antioxidant, aortic oxidant, asymmetric dimethyl arginine, interleukin 1beta, lipid profile, plasma estrogen, von Willebrand factor, lipoxin A4, and erythrocyte membrane fatty acids. The inflammatory and oxidant parameters were significantly increased in ovariectomized rats, but the administration of M. communis extract (100 mg/kg bw) for about 60 days reduced the treated group values. The leaf extract of M. communis possessed protective effects against endothelial dysfunction and atherosclerosis in ovariectomized rats because of its high amount of antioxidant and anti-inflammatory compounds, including ω-3 fatty acids (Youness et al. 2016).
Hemostatic effect
The application of 5% extract to the cut tail in the bleeding model of rat-tail significantly reduced the time of bleeding (P< 0.001) as compared to normal saline group. The figures of PT and aPTT were elevated >120 s and >180 s by 5% extract, respectively. Likewise, serum proteins and protein precipitation were significantly reduced in the extract group of extract with 5% (Hajiaghaee et al. 2016).
Central nervous effects
The acetone, dichloromethane, ethyl acetate, and methanol extracts (200 μg/ml) of the leaf and berry extracts of M. communis were screened beside butyrylcholinesterase, acetylcholinesterase, and tyrosinase. All these enzymes are linked with neurodegenerative diseases. The extracts exhibited a mild acetylcholinesterase (17.49 ± 3.99% to 43.15 ± 1.55%) and inhibition of tyrosinase (4.48 ± 1.50% to 40.53 ± 0.47%). The leaf extracts were not effective against butyrylcholinesterase, while 21.83 ± 3.82% and 36.80 ± 2.00% were the inhibitions demonstrated by the berry extracts (Romani et al. 2004).
Myrtle’s ability to protect the neuron in rats was studied against neurotoxicity induced with lipopolysaccharides (LPS), malondialdehyde, tumor necrosis factor α, nitric oxide, interleukin1β, Willebrand factor (VWF), asymmetric dimethyl arginine (ADMA), estrogen, 5LOX, 15LOX, and lipoxin A4. The tissue and serum of challenged rat’s brain were examined. The outcomes showed that investigated stress parameters significantly increased while estrogen level significantly decreased in rats intoxicated with LPS. Notable improvement was discovered in every studied biomarker (Shahmohammadi et al. 2012).
The central nervous effects of 80% M. communis leaf ethanolic extract (25–400 mg/kg, ip) were evaluated in mice and rats. Mice were subjected to grip strength, pentylenetetrazol-induced seizure, open field, and righting reflex assessments. Male rats were assessed for rapid eye movement (REM) as well as non-REM (NREM) sleep alterations. M. communis extract (50 – 200 mg/kg) assessment was done for horizontal activity (ED50 = 251 ± 55 mg/kg) and vertical activity (ED50 = 40.2 ± 6.6 mg/kg), while treatment with 200 and 400 mg/kg mitigated significant muscle tone in a method that is dependent on the dose. An important hypnotic, but no anticonvulsant effect, was recorded on administration with the extract at 200 mg/kg (P< 0.01). The reduced REM time of sleeping was shown by the electroencephalography results, while the NREM and total sleep times were significantly elevated compared to the mice control group (Aykac et al. 2019).
The mouse model was examined for the sedative-hypnotic effect of the aqueous extract. In the myrtle aqueous extract 200 mg/kg, the locomotor activity was significantly reduced in open field test (P<0.01). The hypnotic effect of aqueous extract of myrtle showed significance and was recorded in righting loss reflex test induced with pentobarbital induced (P <0.05) (EL-Kholy et al 2018). The efficacy of M. communis treatment on the factors that play a vital role in Alzheimer’s disease pathogenesis was compared with galantamine administration. The level of expression of muscarinic receptors M1, AchE, Ach activity, BDNF, GSH level, MPO, and MDA activity, and the gene expression of AchE were examined in rats induced with scopolamine. The results revealed that, M. communis administration in treatment significantly improved the reduction of latency in scopolamine-induced rats and object recognition time, elevating M1, BDNF, and Ach receptor levels of expression in the various regions of the brain. Furthermore, M. communis administration exhibited elevated AchE by improving GSH activity and decreasing MPO activity and MDA level (Naji et al. 2018).
Protective effects
M. communis extract (300mg/kg bw, daily for 49 days) was studied for its protective effect against acrylamide and monosodium glutamate-induced hepatotoxicity in male rats. The results verified that the administration of monosodium glutamate and acrylamide lead to a significant elevation in LDL-C, TC, TL, TG, GGT, ALT, AST, ALP, TB, and MDA. However, myrtle leaves extract caused noticeable decrease in TP, GSH, Alb, TAC, CAT, SOD, GSH-Px, and HDL-C (Sen et al. 2016).
M. communis extract ameliorative effect was assessed on the arsenic chloride (AsCl3) toxicity-inducing tumor suppressor protein (P53) production in rats. The results showed that AsCl3 induced negative effects on the levels of P53-based gene expression and possibly P53 gene protein activity, bringing about low expression of gene. However, M. communis improved the status by elevating P53-based gene expression levels if used alone or mixed with AsCl3 (Samareh et al. 2018).
M. communis sp. was also examined for its antifibrotic and antioxidant effects against hepatic fibrosis and injury arising from biliary obstruction in rats. Direct bilirubin, plasma total bilirubin, alanine aminotransferase, aspartate aminotransferase, interleukin-1β levels, tumor necrosis factor-α were significantly elevated in the group with bile duct ligation, while these figures significantly declined in the bile duct ligation group administered with M. communis sp. Communis extract. Superoxide dismutase and glutathione were reduced significantly in the bile duct ligation group as compared to the groups in the control but increased significantly in extract-treated group. The levels of tissue luminol, myeloperoxidase activity, malondialdehyde, transforming growth factor-beta, lucigenin, and hydroxyproline increased dramatically in the bile duct ligation and were reduced in the group treated with the extract (Ozbeyli et al. 2020).
The therapeutic and protective effects of M. communis methanolic extracts (50 mg/kg/day, intraperitoneal ip, from days 0 to 13) and (50 mg/kg, ip, from 14 to 27 days), respectively, of the methanolic extract of M. communis were studied in rats against bleomycin-induced pulmonary fibrosis. Parenchymal inflammation and fibrotic changes, lipid peroxidation, and hydroxyproline content were significantly decreased in the myrtle extract-treated group, but catalase activity was greater. An enhancement in fibrosis and inflammation was seen in myrtle-treated group, particularly in the initial fibrosis phase (preventive regime) (Mahboubi et al. 2016).
M. communis ssp. leaf ethanol extract protective effect administration (for 14 days before induction) was studied against acute pancreatitis induced with cerulenin in rats. Pancreatic damage induced by cerulenin was related with elevated serum activity of amylase and lipase, the pancreatic activity, level of myeloperoxidase, malondialdehyde, interleukin-1β, and interleukin-6. Acute pancreatitis similarly manifested by a decline in the levels of anti-inflammatory interleukin-10 and glutathione in the pancreas. In the extract pretreatment group, before the induction of pancreatitis, the pancreatic injury detected through the histological analysis was significantly reduced, including a reversal in biochemical changes (Zohalinezhad et al. 2016).
Gastrointestinal effects
M. communis essential oil and its decoction reduced the average time of pain and lessened ulcers size in patients with slight recurring aphthous stomatitis, showing no antagonistic effects. All the patients were content with M. communis topical essential oil (5%); 81% of patients preferred M. communis topical decoctions (5%). The effectiveness of M. communis was linked to its antiseptic, anti-inflammatory, analgesic, and its wound healing effects (Jabri et al. 2016a, b, c).
The aqueous seed extract of myrtle berry’s protective effects against induced esophageal reflux was studied in rats. The induced esophageal reflux caused noticeable histopathological and macroscopic variations in the esophagus. It also followed oxidative stress as measured by a rise in lipid peroxidation, a reduction of the sulfhydryl groups, and levels of glutathione, including the depletion of antioxidant enzyme activities. The extract nullified all biochemical, histopathological, and morphological changes. The induced esophageal reflux also amplified esophageal calcium, free iron levels, and hydrogen peroxide, while extract management protected compared to intracellular mediator’s deregulation (Bouzabata 2013).
M. communis effects in gastroesophageal reflux disease were investigated in comparison with omeprazole through 42 days double-blind randomized controlled scientific trial. About 45 applicants were randomly given three groups: myrtle berries freeze-dried aqueous extract (1000 mg/day), omeprazole capsules (20 mg/day), and the third group received a combination of both treatments. The dyspeptic and reflux scores decreased significantly in all groups when compared with the respective standards. However, substantial changes existed in myrtle extract group (acid reflux and dysmotility-like symptoms related scores) (Benchikh et al. 2016).
The anti-gastric ulcer effect of M. communis dried berry aqueous extract (105 and 175 mg/kg, orally) and methanolic extract (93 and 154 mg/kg, orally) was studied against rats induced with pyloric ligation, ethanol, and indomethacin. Aqueous extract administered orally (at both doses) caused the ulcer index in all prototypes of ulcers to decrease significantly. The aqueous extract (small dose) and methanolic extract (high dose) of M. communis demonstrated effects that were more significant than those of omeprazole in ulcer models induced with ethanol. Two doses of extracts lessened gastric juice volume and total acidity but elevated gastric wall mucus content and gastric pH in the ulcer’s models. Histopathological studies of rat’s gastric tissues administered with methanolic and aqueous extracts in ulcer induced with indomethacin exhibited a significant protective effect against ulcer at that dose level (Sumbul et al. 2012).
The effects of M. communis berry juice (5 and 10 ml/kg bw, orally) on gastric emptying and normal gastro-intestinal transit, including diarrhea induced by castor oil, oxidative stress in the small intestine, and entero-pooling tests, were studied in rats. The juice inhibited intestinal motility and gastric emptying in a dose-dependent manner. The juice administration equally caused a significant dose-dependent defense against diarrhea and intestinal fluid buildup. The status of oxidative stress in the intestine associated with intestinal hypersecretion induced with castor oil was attenuated via juice administration (Jabri et al. 2016a, b, c).
Antidiarrheal effects
In mice, model-treated M. communis essential oils were examined for their antidiarrheal and antisecretory activities. The M. communis oil [abundant in 1.8-cineole 26.5% and α-pinene 54.1%] reduced significantly the gastric emptying dose of 500 mg/kg and the intestinal transfer at the entire doses utilized (50, 250, and 500 mg/kg). The extract essential oil similarly possessed anti-diarrheal and anti-secretory action depending on the dose (Sisay et al. 2017).
The antidiarrheal effects of an aqueous extract of M. communis berry seeds were studied in diarrhea induced with castor oil in rats. The defense of the extract depended on the dose against intestinal fluid and diarrhea buildup. Hypersecretion of the intestine induced with castor oil was complemented by the intestinal status of oxidative stress; hydrogen peroxide in the intestine also increased, as did free iron and calcium levels; however, the group pre-treated with the extract showed alteration in all castor oil-induced intracellular mediators’ turbulence. The results also showed that the extract has abundant total and condensed tannins and presents antibacterial action in a broad spectrum (Panahi et al. 2014).
The methanol extracts of 200 mg/kg (P< 0.05) and 400 mg/kg (P< 0.01), including the methanol and chloroform fractions of 400 mg/kg (P< 0.05), all significantly hindered the commencement of diarrhea. All doses of methanol extract, and both segments at 300 and 400 mg/kg, expressively lessened the regularity and mass of fecal yields. Charcoal meal test output showed the methanol extract at all prescriptions (P< 0.001) as well as the two fractions of 300 mg/kg (P< 0.05) and 400 mg/kg produced a significant (P< 0.001) anti-motility effect. In the entero-pooling test, the methanolic extract at all verified prescriptions (P< 0.01), and the fractions at 300 mg/kg and 400 mg/kg (P< 0.05) produced a notable decrease in the capacity and mass of intestinal substances (Mahboubi et al. 2017).
Anti-hemorrhoid effects
The efficiency of topical cream from the essential oil M. communis in alleviating hemorrhoids symptoms compared to ointments that are anti-hemorrhoid (containing zinc oxide, lidocaine, hydrocortisone, and aluminum subacetate) was evaluated in a random double-blind double-dummy trial performed on 106 patients. All assessed indications (long-lasting pain, bleeding, pain during excretion, and irritation, anus itching, tenesmus, and heaviness) were considerably diminished by trial end (P<0.001). No remarkable difference was noticed in the level of improvement of evaluated symptoms concerning ointment of anti-hemorrhoid and M. communis (P>0.05) (Malekuti et al. 2019).
The therapeutic effect of the essential oil of M. communis on hemorrhoids management was clinically examined. The results pointed out that the essential oil of M. communis (cream or ointment) significantly ameliorated hemorrhage, excretion pain, perpetual pain, anal itching, anal irritation, and anal weightiness in types I and II hemorrhoids’ patients. M. communis was dynamic in patients’ treatment, not responding to organic product usages (anti-hemorrhoids cream) (Janbaz et al. 2013).
A triple-blind random controlled trial was done to regulate the anti-hemorrhoid effect of M. communis ointments (two times a day, every single 12 ± 2 h, through the rectum for 28 days) compared with anti-hemorrhoid ointment, taking the life quality and hemorrhoid into consideration (main outcomes) with contentment of the management and harmful effects (minor results) in females with grade I and II hemorrhoid. The colorectal assessment of a clinical therapeutics gauge (CORECTS) was utilized to measure the severity of hemorrhoid and the World Health Organization Life Quality Survey (WHOQOL-BREF) to decide the value of life. The harshness of hemorrhoid symptoms was reduced in both groups, showing no significant difference statistically between both groups (P>0.05). The anal itching mean was significantly lower 28–56 days after involvement in the M. communis cream group (P<0.05). No significant difference was seen between groups at 28–56 days after considering life quality (P>0.05). The participants given M. communis cream remained significant, showing satisfaction in the drug usage (Harassi et al. 2019).
Effects on smooth muscles
M. communis crude methanol extract triggered full contractions induced by K+ (80 mM) that are also spontaneous in isolated rabbit jejunum. It induced right-side shift similar to response curves of calcium. M. communis crude methanol extract also caused relaxation on K+ (80 mM)-induced contractions in isolated rabbit tracheal arrangements and caused phenylephrine relaxation (1 μM) and K+ (80 mM)-induced contractions in isolated rabbit aorta preparations, like verapamil, a standard calcium channel blocker (Ozturk and Duran 2018).
Anticancer and antimutagenic effects
The cytotoxicity of M. communis oils was investigated against MCF-7 and P815 cells. P815 cells showed less activity than MCF-7 cells, with IC50 values of 4–6.25 µg/ml against the essential oils. Cell toxicity is related to DNA fragmentation, an important apoptosis hallmark (Tretiakova et al. 2008).
The cytotoxic effect of water, ethanol, methanol, n-hexane, and ethyl acetate extracts of M. communis leaves was assessed in vitro against brine shrimp. All the extracts showed cell toxicity against brine shrimp (LC50 < 1000). The extracts of n-hexane extracts and ethyl acetate showed additional cytotoxic activity compared to other extracts (Fani et al. 2014). The essential oil of M. communis antiproliferative effect was studied against cancer Vero cells and HCT 116. M. communis IC50 values were examined at 25 μg/ml in HCT 116 and 15 μg/ml in Vero cells (Hayder et al. 2008).
The anticancer potential of myrtucommuacetalone-1 (MCA-1), isolated from M. communis, was assessed with an MTT cytotoxic assay. Cytotoxicity investigations of MCA-1 on 3T3 mouse fibroblasts, CC1 liver cell line, J774.2, macrophages, and MDBK bovine kidney epithelial cell revealed IC50 values of 6.53 ± 1.2, 4.6 ± 0.7, 5 ± 0.8, and 4.6 ± 0.7, µg/ml, respectively (Maxia et al. 2011).
Myrtucommulone strongly induced the death of cells of diverse cancer cell lines (EC50 3-8 microM); apoptosis was visualized by stimulating cleavage of poly (ADP-ribose) polymerase (PARP) cleavage, caspase-3, -8, and -9, DNA fragmentation, and nucleosomes release into the cytosol. It was not cytotoxic against foreskin fibroblasts with EC50 of cell death around 20–50 microM or non-transformed human peripheral blood mononuclear cells (PBMC). Furthermore, its apoptotic effects were facilitated by the intrinsic, not extrinsic death pathway. It triggered the reduction of mitochondrial membrane potential in MM6 cells, and mitochondria cytochrome c release is induced (Choudhary et al. 2013).
M. communis aqueous, chloroform, ethyl acetate, methanol, hexane, total flavonoids oligomer fraction, and essential oil significantly reduced the response to DNA damage caused by nifuroxazide and aflatoxin B1 in a system of bacterial assays (Escherichia coli PQ 37 with the SOS chromotest). The extracts of methanol and ethyl acetate displayed strong inhibition against DNA damage response caused by secondary genotoxic aflatoxin B1 (Ghadami et al. 2014).
M. communis oils antimutagenic properties were assessed against reactive mutagenesis induced by t-BOOH in Escherichia coli oxyR mutant IC 202, (a bacterial strain) unable to eliminate ROS. A decline in reactive mutagenesis occurred when myrtle oil was administered with the highest concentration at 13%. During the use of oxidative mutagen, the oil greatly reduced mutagenesis in a manner dependent on the concentration (at the highest concentration of 28%) (Cottiglia et al. 2012).
Immunomodulatory effect
Myrtucommuacetalone, myricetin, myrtucommulone M, myrtucommulone E isousnic acid, and growth regulator G3 factor are all M. communis isolates assessed for their ability to lessen the immune response through their effects on various immune system constituents. Compounds of myrtucommuacetalone and growth regulator G3 factor were significantly inhibited against nitric oxide (NO) production. Myrtucommuacetalone also revealed important antiproliferation (IC50< 0.5 μg/ml) against proliferation of T-cell. Significant inhibition was caused by myricetin (IC50 = 1.6 μg/ml) on the blood phagocytes stimulated by zymosan in ROS generation. The compounds myrtucommuacetalone and myricetin were active against ROS generation stimulated by PMA (Ozcan et al. 2019).
Dermatological effects
M. communis can be applied topically in wart treatment. It displayed an additional rapid response compared to salicylic acid and very few side effects (Salehpour et al. 2016).
The protective effect of oral or topical M. communis management (100 mg/kg per day) either topically or orally for 2 days was investigated against rats induced with burn damage (in the burn group, dorsum was clean-shaven and kept in water bath with a temperature of about 90 °C). The burn injury in the skin was triggered by heat, causing a significant reduction in glutathione and nitric oxide levels and activities of catalase, superoxide dismutase, and tissue factor, followed by a significant increase in skin malondialdehyde levels. Myrtle management altered every biochemical index and also caused histopathological alterations as a result of heat trauma. The topical and oral administration of myrtle extract possessed an ameliorative role in the oxidative damage induced by the wound (Hayder et al. 2008).
Toxicity and side effects
The ethanolic and aqueous extracts of aerial parts of M. communis showed LD50 values of 0.79 and 0.473 g/kg in mice separately (Soomro et al. 2019).
In Arabi sheep, blood parameters were assessed for the effect of M. communis leaf on them. The result showed significant reduction in the glucose, triglyceride, and blood urea of sheep when fed a diet containing 4% myrtle leaf compared with the control diet (D’Urso et al. 2017).
Recent studies that elaborated on various aspects of the therapeutic potential of M. communis include Dabbaghi et al. (2023) that states that myrtle’s strong antioxidant concentration is one of its most important defensive qualities. Research have demonstrated that myrtle’s antioxidant qualities can offer defense against dangerous compounds like pesticides, heavy metals, and other pollutants found in the environment. Furthermore, myrtle possesses anti-inflammatory qualities that may lessen the harm brought on by prolonged exposure to pollutants. Myrtle’s anti-inflammatory and antibacterial qualities have also shown promise in treating gastrointestinal disorders like stomach ulcers.
In fact, product from M. communis is getting closer to the clinic as a randomized clinical trial was carried out to assess the therapeutic effects of M. communis on chromic skin lesions, and they measured the duration of sleep, number of nighttime awakenings, quality of life, and chronic skin problems and itching-related characteristics (such as the itching time, severity, distribution, frequency, and calculated itching score) in the two groups. Applying myrtle cream effectively reduced skin issues, such as itching and burning feeling, according to our data research. Furthermore, when compared to pre-treatment, myrtle significantly reduced skin lesion symptoms such as excoriation in the case group. Myrtle cream was found to have a notable positive impact on the patients’ quality of life within the case group. More detailed information about the preventive effect of myrtle against skin complications caused by sulfur mustard is provided by this study. Additionally, myrtle significantly raised the veterans’ quality of life who had been exposed to sulfur mustard (Iman et al. 2022).
Conclusion
M. communis is used at different places throughout the world as a traditional herbal remedy. It contained many bioactive ingredients and possessed a wide range of pharmacological effects like anti-inflammatory, analgesic, antimicrobial, antiparasitic, antioxidant, antidiabetic, cardiovascular, central nervous, protective, gastrointestinal, anticancer, antimutagenic, immunomodulatory, dermatological, and many other biological effects. According to the data on in vitro and in vivo studies, M. communis has the potential to be used in pharmaceutical development as a medicine for wound healing, treatment of gastrointestinal disorders, and cancer management, to name a few, because of its safety and effectiveness.
Data availability
Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.
References
Aidi Wannes W, Mhamdi B, Sriti J, Ben Jemia M, Ouchikh O, Hamdaoui G, Kchouk ME, Marzouk B (2010) Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food Chem Toxicol. 48(5):1362–1370
Aleksic V, Knezevic P (2013) Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiol Res. 168(6):311–332
Aleksic V, Mimica-Dukic N, Simin N, Nedeljkovic NS, Knezevic P (2014) Synergistic effect of Myrtus communis L. essential oils and conventional antibiotics against multi-drug resistant Acinetobacter baumannii wound isolates. Phytomedicine. 21(12):1666–1674
Alem G, Mekonnen Y, Tiruneh M, Mulu A (2008) In vitro antibacterial activity of crude preparation of myrtle (Myrtus communis) on common human pathogens. Ethiop Med J. 46(1):63–69
Alipour G, Dashti S, Hosseinzadeh H (2014) Review of pharmacological effect of Myrtus communis L. and its active constituent. Phytother Res 28:1125–1136
Al-Jeboory A, Abdul L, Jawad M (1985) Cardiovascular and antimicrobial effects of Myrtus communis. Indian J Pharmacol. 17:233–235
Al-Snafi AE (2013) Chemical constituents and pharmacological activities of milfoil (achillea santolina)—a review. Int J Pharm Tech Res 5(3):1373–1377
Al-Snafi AE (2013) The pharmaceutical importance of Althaea officinalis and Althaea rosea: a review. Int J Pharm Tech Res 5(3):1387–1385
Al-Snafi AE (2014) The pharmacology of Anchusa italica and Anchusa strigosa—a review. Int J Pharm Pharm Sci 6(4):7–10
Al-Snafi AE (2014) The pharmacological importance of Anethum graveolens—a review. Int J Pharm Pharm Sci 6(4):11–13
Al-Snafi AE (2016) Immunological effects of medicinal plants: a review (part 2). Immunol Endocr Metab Agents Medic Chem 16(2):100–121
Al-Snafi AE (2018) Pharmacological and therapeutic importance of Hibiscus sabdariffa—a review. Int J Pharm Res 10(3):451–475
Al-Snafi AE (2021) Chemical constituents and pharmacological effects of Ocimum basilicum—a review. Int J Pharm Res 13(2):2997–3013
Al-Snafi AE, Ibraheemi ZAM, Talab TA (2021) A review on components and pharmacology of Mangifera indica. Int J Pharm Res 13(2):3043–3066
Amel B (2015) Screening of the antioxidant and hypotensive activities of some medicinal plants in relation to their polyphenolic contents. PhD thesis, Université Ferhat Abbas Sétif 1, Faculté des Sciences de la Nature et de la Vie
Amensour M, Sendra E, Abrini J, Bouhdid S, Pérez-Alvarez JA, Fernández-López J (2009) Total phenolic content and antioxidant activity of myrtle (Myrtus communis) extracts. Nat Prod Commun. 4(6):819–824
Amiri K, Nasibi S, Mehrabani M, Nematollahi MH, Harandi MF (2019) In vitro evaluation on the scolicidal effect of Myrtus communis L. and tripleuros permum disciforme L methanolic extracts. Exp Parasitol. 199:111–115
Andrea M, Noemi T, Graziano G, Mario D, Enrico S, Daniele P, Urska V, Fulvio M, Manca G (2019) Myrtle seeds (Myrtus communis L.) as a rich source of the bioactive ellagitannins oenothein B and eugeniflorin D2. ACS Omega 4:15966–15974
Appendino G, Maxia L, Bettoni P, Locatelli M, Valdivia C, Ballero M, Stavri M, Gibbons S, Sterner O (2006) Antibacterial galloylated alkylphloroglucinol glucosides from myrtle (Myrtus communis). J Nat Prod. 69(2):251–254
Aslam S, Ganaie KA, John AQ, Dar GH (2010) Family myrtaceae in Kashmir Myrtus communis L.—a new record for the shrub world of Kashmir Himalayas. Academia Arena. 2(5):42–43
Aykac A, Ozbeyli D, Uncu M, Ertaş B, Kılınc O, Şen A, Orun O, Sener G (2019) Evaluation of the protective effect of Myrtus communis in scopolamine-induced alzheimer model through cholinergic receptors. Gene. 689:194–201
Barac A, Donadu M, Usai D, Spiric VT, Mazzarello V, Zanetti S, Aleksic E, Stevanovic G, Nikolic N, Rubino S (2018) Antifungal activity of Myrtus communis against malassezia sp. isolated from the skin of patients with pityriasis versicolor. Infection. 46(2):253–257
Barboni T, Cannac M, Massi L, Perez-Ramirez Y, Chiaramonti N (2010) Variability of polyphenol compounds in Myrtus communis L. (myrtaceae) berries from Corsica. Molecules 15:7849–7860
Batiha GES, Tene ST, Teibo JO et al (2023a) The phytochemical profiling, pharmacological activities, and safety of malva sylvestris: a review. Naunyn-Schmiedeberg’s Arch Pharmacol 396:421–440. https://doi.org/10.1007/s00210-022-02329-w
Batiha GES, Teibo JO, Shaheen HM et al (2023b) Therapeutic potential of Lawsonia inermis Linn: a comprehensive overview. Naunyn-Schmiedeberg’s Arch Pharmacol. https://doi.org/10.1007/s00210-023-02735-8
Baz H, Gulaboglu M, Gozcu L, Demir GM, Canayakin D, Suleyman H, Halici Z, Baygutalap NK (2016) Effects of aqueous extract of Myrtus communis L. leaves on streptozotocin-induced diabetic rats. J Res Med Dental Sci 4(3):214–218
Ben Hsouna A, Hamdi N, Miladi R, Abdelkafi S (2014) Myrtus communis essential oil: chemical composition and antimicrobial activities against food spoilage pathogens. Chem Biodivers. 11(4):571–580
Benchikh F, Benabdallah H, Dahamna S, Khennouf S, Flamini G, Amira S (2016) Antimotility and andidiarrhoel activity of Myrtus communis L. leaves essential oil in mice. Intern J Pharmacog Phytochem Res 8(7):1238–1244
Beni AS, Shahmokhtar MK, Masoumiasl A, Khajehsharifi H (2017) Phytochemical and biological studies of some myrtus (Myrtus communis L.) populations of south west region of zagros (iran). Nat Prod Chem Res. 5:7
Berka-Zougali B, Ferhat MA, Hassani A, Chemat F, Allaf KS (2012) Comparative study of essential oils extracted from algerian Myrtus communis L. leaves using microwaves and hydrodistillation. Int J Mol Sci. 13(4):4673–4695
Bouaoudia-Madi N, Boulekbache-Makhlouf L, Kadri N, Dahmoune F, Remini H, Dairi S, Oukhmanou-Bensidhoum S, Madani K (2017) Phytochemical analysis of Myrtus communis plant: conventional versus microwave assisted-extraction procedures. J Complement Integr Med. 14(4). https://doi.org/10.1515/jcim-2016-0098
Bouaziz A, Khennouf S, Abuzarga M, Abdalla S, Baghiani A, Charef N (2015) Phytochemical analysis, hypotensive effect and antioxidant properties of Myrtus communis L. growing in Algeria. Asian Pacific J Tropic Biomed 5(1):19–28
Boudjelal A, Henchiri C, Sari M, Sarri D, Hendel N, Benkhaled A, Ruberto G (2013) Herbalists and wild medicinal plants in M’sila (north Algeria): an ethnopharmacology survey. J Ethnopharmacol. 148:395–4022
Bouzabata A (2013) Traditional treatment of high blood pressure and diabetes in Souk Ahras District. J Pharmacogn Phytother 5:12–20
Bouzabata A, Cabral C, Gonçalves MJ, Cruz MT, Bighelli A, Cavaleiro C, Casanova J, Tomi F, Salgueiro L (2015) Myrtus communis L. as source of a bioactive and safe essential oil. Food Chem Toxicol. 75:166–172
Cannas S, Molicotti P, Ruggeri M, Cubeddu M, Sanguinetti M, Marongiu B, Zanetti S (2013) Antimycotic activity of Myrtus communis L. towards Candida spp. from clinical isolates. J Infect Dev Ctries. 7(3):295–298
Cannas S, Molicotti P, Usai D, Maxia A, Zanetti S (2014) Antifungal, anti-biofilm and adhesion activity of the essential oil of Myrtus communis L. against Candida species. Nat Prod Res. 28(23):2173–2177
Chegeni TN, Ghaffarifar F, Khoshzaban F, Asl AD, Mirzaian H, Jameie F (2019) Effects of aqueous and ethanolic extracts of Myrtus communis leaves on trophozoites and cysts of Acanthamoeba: an in vitro study. Int J Herb Med Labor 6(3):219–225
Choudhary MI, Khan N, Ahmad M, Yousuf S, Fun HK, Soomro S, Asif M, Mesaik MA, Shaheen F (2013) New inhibitors of ROS generation and T-cell proliferation from Myrtus communis. Org Lett. 15(8):1862–1865
Cottiglia F, Casu L, Leonti M, Caboni P, Floris C, Busonera B, Farci P, Ouhtit A, Sanna G (2012) Cytotoxic phloroglucinols from the leaves of Myrtus communis. J Nat Prod. 75(2):225–229
Cruciani S, Santaniello S, Garroni G, Fadda A, Balzano F, Bellu E, Sarais G, Fais G, Mulas M, Maioli M (2019) Myrtus polyphenols, from antioxidants to anti-inflammatory molecules: exploring a network involving cytochromes P450 and vitamin D. Molecules. 24(8):1515
D’Urso G, Sarais G, Lai C, Pizza C, Montoro P (2017) LC-MS based metabolomics study of different parts of myrtle berry from Sardinia (italy). J Berry Res. 7:217–229
Dabbaghi MM, Fadaei MS, Roudi SH, Baradaran Rahimi V, Askari VR (2023) A review of the biological effects of Myrtus communis. Physiol Rep 11(14):e15770. https://doi.org/10.14814/phy2.15770
Dairi S, Madani K, Aoun M, Him JL, Bron P, Lauret C, Cristol JP, Carbonneau MA (2014) Antioxidative properties and ability of phenolic compounds of Myrtus communis leaves to counteract in vitro LDL and phospholipid aqueous dispersion oxidation. J Food Sci. 79(7):C1260-1270
Dellaoui H, Berroukche A, Halla N, Boudaoud L, Terras M (2018) Phytochemical study and evaluation of the antioxidant of Myrtus communis L. fruit’s methanolic extract. Phyto Chem Bio Sub J 12(2):100–109
Demir GM, Gulaboglu M, Aggul AG, Baygutalp NK, Canayakin D, Halici Z, Suleyman H (2016) Antioxidant and antidiabetic activity of aqueous extract of Myrtus communis L. berries on streptozotocin-induced diabetic rats. IOSR J Pharm Biol Sci (IOSR-JPBS). 11(5):11–16
Dragomanova S, Tancheva L, Georgieva M, Klisurov R (2019) Analgesic and anti-inflammatory activity of monoterpenoid myrtenal in rodents. J IMAB. 25(1):2406–2413
Ebrahimi F, Mahmoudi J, Torbati M, Karimi P, Valizadeh H (2020) Hemostatic activity of aqueous extract of Myrtus communis L. leaf in topical formulation: in vivo and in vitro evaluations. J Ethnopharmacol 249:112398
Elfellah MS, Akhter MH, Khan MT (1984) Anti-hyperglycaemic effect of an extract of Myrtus communis in streptozotocin-induced diabetes in mice. J Ethnopharmacol. 11(3):275–281
EL-Kholy WM, EL-Sawi MRF, Galal NA (2018) (2018) effect of Myrtus communis extract against hepatotoxicity induced by monosodium glutamate and acrylamide in male rats. Egypt J Hosp. 70(9):1676–1681
Fadil M, Fikri-Benbrahim K, Rachiq S, Ihssane B, Lebrazi S, Chraibi M, Haloui T, Farah A (2018) Combined treatment of Thymus vulgaris L., Rosmarinus officinalis L. and Myrtus communis L. essential oils against salmonella typhimurium: optimization of antibacterial activity by mixture design methodology. Eur J Pharm Biopharm. 126:211–220
Fani MM, Kohanteb J, Araghizadeh A (2014) Inhibitory activity of Myrtus communis oil on some clinically isolated oral pathogens. Med Princ Pract. 23(4):363–368
Feisst C, Franke L, Appendino G, Werz O (2005) Identification of molecular targets of the oligomeric nonprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds. J Pharmacol Exp Ther. 315(1):389–396
Feuillolay C, Pecastaings S, Le Gac C, Fiorini-Puybaret C, Luc J, Joulia P, Roques C (2016) A Myrtus communis extract enriched in myrtucummulones and ursolic acid reduces resistance of Propionibacterium acnes biofilms to antibiotics used in acne vulgaris. Phytomedicine. 23(3):307–315
Flaminia G, Cionia PL, Morellia I, Maccionib S, Baldini R (2004) Phytochemical typologies in some populations of Myrtus communis L. on caprione promontory (east Liguria, Italy). Food Chem 85:599–604
Franco AM, Tocci N, Guella G, Dell’Agli M, Sangiovanni E, Perenzoni D, Vrhovsek U, Mattivi F, Manca G (2019) Myrtle seeds (Myrtus communis L.) as a rich source of the bioactive ellagitannins oenothein B and eugeniflorin D2. ACS Omega. 4(14):15966–15974
Gaber El-Saber B, Hussein DE, Algammal AM, George TT, Jeandet P, Al-Snafi AE, Tiwari A, Pagnossa JP, Lima CM, Thorat ND, Zahoor M, El-Esawi M, Dey A, Alghamd S, Hetta HF, Cruz-Martins N (2021) Antimicrobials in food preservation: recent views. Food Control 126:108066
Ghadami Yazdi E, Minaei MB, Hashem Dabaghian F, Ebrahim Zadeh Ardakani M, Mohammad Ranjbar A et al (2014) Efficacy of Myrtus communis L. and Descurainia sophia L versus salicylic acid for wart treatment. Iran Red Crescent Med J. 16(10):e16386
Hajiaghaee R, Faizi M, Shahmohammadi Z, Abdollahnejad F, Naghdibadi H, Najafi F, Razmi A (2016) Hydroalcoholic extract of Myrtus communis can alter anxiety and sleep parameters: a behavioural and EEG sleep pattern study in mice and rats. Pharm Biol. 54(10):2141–2148
Harassi Y, Tilaoui M, Idir A, Frédéric J, Baudino S, Ajouaoi S, Mouse HA, Zyad A (2019) Phytochemical analysis, cytotoxic and antioxidant activities of Myrtus communis essential oil from Morocco. J Complement Integr Med. 16(3). pii: /j/jcim
Harassi Y, Tilaoui M, Idir A, Frédéric J, Baudino S, Ajouaoi S, Mouse HA (2019) Myrtus communis essential oil from Morocco. J Complement Integr Med. 16(3):pii: /j/jcim
Hayder N, Skandrani I, Kilani S, Bouhlel I, Abdelwahed A, Ben Ammar R, Mahmoud A, Ghedira K, Chekir-Ghedira L (2008) Antimutagenic activity of Myrtus communis L. using the Salmonella microsome assay. South Afr J Botany. 74:121–125
Hosseinzadeh H, Khoshdel M, Ghorbani M (2011) Antinociceptive, anti-inflammatory effects and acute toxicity of aqueous and ethanolic extracts of Myrtus communis L. aerial parts in mice. J Acupunct Meridian Stud 4(4):242–247
Iman Maryam, Houri Edalat, Seyyed Masoud Davoudi, Seyyedeh Hamideh Molaei, Zahra Bahari. A randomized clinical trial on therapeutic effects of Myrtus communis L. cream on chronic skin lesions and quality of life of sulfur mustard-exposed Veterans Maryam. Vol.65: e22210268, 2022 https://doi.org/10.1590/1678-4324-2022210268 ISSN 1678-4324 Online Edition Brazilian Archives of Biology and Technology. Vol.65: e22210268, 2022 www.scielo.br/babt
Ines S, Ines B, Wissem B, Mohamed BS, Nawel H, Dijoux-Franca MG, Kamel G, Leïla CG (2012) In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2. J Appl Toxicol. 32(5):333–341
Jabri MA, Tounsi H, Rtibi K, Marzouki L, Sakly M, Seba H (2016a) Ameliorative and antioxidant effects of myrtle berry seed (Myrtus communis) extract during reflux-induced esophagitis in rats. Pharm Biol 54(9):1575–1585
Jabri MA, Rtibi K, Sakly M, Marzouki L, Sebai H (2016b) Role of gastrointestinal motility inhibition and antioxidant properties of myrtle berries (Myrtus communis L.) juice in diarrhea treatment. Biomed Pharmacother. 84:1937–1944
Jabri MA, Rtibi K, Ben-Said A, Aouadhi C, Hosni K, Sakly M, Sebai H (2016c) Antidiarrhoeal, antimicrobial and antioxidant effects of myrtle berries (Myrtus communis L.) seeds extract. J Pharm Pharmacol. 68(2):264–274
Jabri MA, Marzouki L, Sebai H (2018) Ethnobotanical, phytochemical and therapeutic effects of Myrtus communis L. berries seeds on gastrointestinal tract diseases: a review. Arch Physiol Biochem 124(5):390–396. https://doi.org/10.1080/13813455.2017.1423504
Janbaz KH, Nisa M, Saqib F, Imran I (2013) Zia-ul-haq M and de feo V bronchodilator, vasodilator and spasmolytic activities of methanolic extract of Myrtus communis L. J Physiol Pharmacol. 64(4):479–484
Javadi F, Azadmehr A, Jahanihashemi H, Adineh M, Nozari S, Hajiaghaee R, Shahnazi M, Saraei M (2017) Study on anti-Toxoplasma effects of Myrtus communis and Artemisia aucheri Boiss extracts. Int J Herb Med 5(4):16–19
Karimlar S, Naderi A, Mohammadi SF, Moslehishad M, Delrish E, Aghajanpour L, Khoshzaban A, Lashay A (2019) Hypoglycemic and hypolipidemic effects of Myrtus communis, Trachyspermum copticum and Ferula gummosa essential oils on streptozotocin induced diabetic rats. Nutr Food Sci Res 6(1):1–8
Khan N, Rasool S, Ali Khan S, Bahadar Khan S. (2019) A new antibacterial dibenzofuran-type phloroglucinol from Myrtus communis linn. Nat Prod Res. 1-6. https://doi.org/10.1080/14786419.2018
Koeberle A, Pollastro F, Northoff H, Werz O (2009) Myrtucommulone, a natural acylphloroglucinol, inhibits microsomal prostaglandin E2 synthase-1. Br J Pharmacol. 156(6):952–961
Kordali S, Usanmaz A, Cakir A, Komaki A, Ercisli S (2016) Antifungal and herbicidal effects of fruit essential oils of four Myrtus communis genotypes. Chem Biodivers. 13(1):77–84
Liang C, Staerk D, Kongstad KT (2019) Potential of Myrtus communis Linn as a bifunctional food: dual high-resolution PTP1B and α-glucosidase inhibition profiling combined with HPLC-HRMS and NMR for identification of antidiabetic triterpenoids and phloroglucinol derivatives. J Funct Foods. 64:103623
Mahboubi M (2016) Myrtus communis L. and its application in treatment of recurrent aphthous stomatitis. J Ethnopharmacol. 193:481–489
Mahboubi M (2017) Effectiveness of Myrtus communis in the treatment of hemorrhoids. J Integr Med. 15(5):351–358
Mahboubi M, Ghazian BF (2010) In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine. 17(10):771–774
Mahdi NK, Gany ZH, Sharief M (2006) Alternative drugs against Trichomonas vaginalis. East Mediterr Health J. 12(5):679–684
Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES (2015) Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L. Korean J Parasitol. 53(1):21–27
Malekuti J, Mirghafourvand M, Samadi K, Abbasalizadeh F and Khodaei L (2019) Comparison of the effect of Myrtus communis herbal and anti-hemorrhoid ointments on the hemorrhoid symptoms and quality of life in postpartum women with grade I and II internal hemorrhoid: a triple-blinded randomized controlled clinical trial. J Complement Integr Med. 2019; 16(4). pii: /j/jcim
Masoudi M, Rafieian Kopaei M, Miraj S (2017) A comparison of the efficacy of metronidazole vaginal gel and myrtus (Myrtus communis) extract combination and metronidazole vaginal gel alone in the treatment of recurrent bacterial vaginosis. Avicenna J Phytomed. 7(2):129–136
Maxia A, Frau MA, Falconieri D, Karchuli MS, Kasture S (2011) Essential oil of Myrtus communis inhibits inflammation in rats by reducing serum IL-6 and TNF-alpha. Nat Prod Commun. 6(10):1545–1548
Medhat D, El-Bana MA, Ashour MN, Badawy E, Diab Y, Hussein J (2017) New approaches in protecting against atherosclerosis in experimental model of postmenopause. J Appl Pharm Sci. 7(11):90–96
Mehrabani M, Kazemi A, Ayatollahi Mousavi SA, Rezaifar M, Alikhah H et al (2013) Evaluation of antifungal activities of Myrtus communis L. by bioautography method. Jundishapur J Microbiol. 6(8):e8316
Mert T, Fafal T, Kivcak B, Ozturk HT (2008) Antimicrobial and cytotoxic activities of Myrtus communis L. J fac pharm. Ankara. 37(3):191–199
Messaoud C, Laabidi A, Boussaid M (2012) Myrtus communis L. infusions: the effect of infusion time on phytochemical composition, antioxidant, and antimicrobial activities. J Food Sci. 77(9):C941-947
Mimica-Dukić N, Bugarin D, Grbović S, Mitić-Culafić D, Vuković-Gacić B, Orcić D, Jovin E, Couladis M (2010) Essential oil of Myrtus communis L. as a potential antioxidant and antimutagenic agents. Molecules. 15(4):2759–27570
Montoro P, Tuberoso CIG, Piacente S, Perrone A, De Feo V, Cabras P, Pizza C (2006) Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. J Pharm Biom Anal. 41:1614–1619
Naji HA, Rhiyf AG, Al-Zebeeby A (2018) Protective features of Myrtus communis leaves against the genotoxic effects of arsenic in wistar rats. J Pharm Sci Res. 10(11):2921–2923
Ozbeyli D, Sen A, Cilingir Kaya OT, Ertas B, Aydemir S, Ozkan N, Yuksel M, Sener G (2020) Myrtus communis leaf extract protects against cerulein-induced acute pancreatitis in rats. J Food Biochem. 44(2):e13130
Ozcan O, Ipekci H, Alev B, Ustundag UV, Ak E, Sen A, Alturfan EE, Sener G, Yarat A, Cetinel S, Akbay TT (2019) Protective effect of myrtle (Myrtus communis) on burn induced skin injury. Burns. 45(8):1856–1863
Ozturk S and Duran N Antiproliferative effects of Origanum syriacum L and Myrtus communis L. On human colon cancer cell line. ICAMS 2018–7th Int Conf Adv Mater Syst. https://doi.org/10.24264/icams-2018.IV.3
Panahi Y, Mousavi-Nayeeni SM, Sahebkar A, Fanaie SA, Rahimnia A, Beiraghdar F (2014) Myrtus communis essential oil for the treatment of hemorrhoids: a randomized double-blind double-dummy parallel-group comparative study. Turk J Pharm Sci. 11(1):1–8
Panjeshahin MR, Azadbakht M, Akbari N (2016) Antidiabetic activity of different extracts of Myrtus communis in streptozotocin induced diabetic rats. Rom J Diabetes Nutr Metab Dis. 23(2):183–190
Penauelas J, Filella I, Ertotognetti R (2001) Leaf mineral concentrations of Erica arborea, Juniperus communis and Myrtus communis growing in the proximity of a natural CO2 spring. Global Change Biol 7:291–301
Petretto GL, Maldini M, Addis R, Chessa M, Foddai M, Rourke JP, Pintore G (2016) Variability of chemical composition and antioxidant activity of essential oils between Myrtus communis var. Leucocarpa DC and var. Melanocarpa DC. Food Chem 197(Pt A):124–131
Pezhmanmehr M, Dastan D, Ebrahimi SN, Hadian J (2010) Essential oil constituents of leaves and fruits of Myrtus communis L. from Iran. J Essent Oil-Bear Plants 13(1):123–129
Qader KO, Al-Saadi SAAM, Al-Saadi TA (2017) Chemical composition of Myrtus communis L. (myrtaceae) fruits. J Appl Life Sci Int 12(3):1–8
Rahimvand L, Niakan M, Naderi NJ (2018) The antibacterial effect of aquatic and methanolic extract of Myrtus communis on Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia. Iran J Microbiol. 10(4):254–257
Raoof M, Khaleghi M, Siasar N, Mohannadalizadeh S, Haghani J, Amanpour S (2019) Antimicrobial activity of methanolic extracts of Myrtus communis L. and Eucalyptus galbie and their combination with calcium hydroxide powder against Enterococcus faecalis. J Dent (Shiraz) 20(3):195–202
Rasaie N, Esfandiari E, Rasouli S, Abdolahian F (2017) Antimicrobial effect of Myrtus communis. L. Essential oils against oral microorganism. Jentashapir J Cell Mol Biol. 9(1):e12032
Romani A, Pinelli P, Mulinacci N, Vincieri F, Tattini M (1999) Identification and quantification of polyphenols in leaves of Myrtus communis. Chromatographia. 49:17–20
Romani A, Coinu R, Carta S, Pinelli P, Galardi C, Vincieri FF, Franconi F (2004) Evaluation of antioxidant effect of different extracts of Myrtus communis L. Free Radic Res. 38(1):97–103
Rosa A, Deiana M, Casu V, Corona G, Appendino G, Bianchi F, Ballero M, Dessì MA (2003) Antioxidant activity of oligomeric acylphloroglucinols from Myrtus communis L. Free Radic Res. 37(9):1013–1019
Rosa A, Melis MP, Deiana M, Atzeri A, Appendino G, Corona G, Incani A, Loru D, Dessì MA (2008) Protective effect of the oligomeric acylphloroglucinols from Myrtus communis on cholesterol and human low density lipoprotein oxidation. Chem Phys Lipids. 155(1):16–23
Rossi A, Di Paola R, Mazzon E, Genovese T, Caminiti R, Bramanti P, Pergola C, Koeberle A, Werz O, Sautebin L, Cuzzocrea S (2009) Myrtucommulone from Myrtus communis exhibits potent anti-inflammatory effectiveness in vivo. J Pharmacol Exp Ther. 329(1):76–86
Rotstein A, Lifshitz A, Kashman Y (1974) Isolation and antibacterial activity of acylphloroglucinols from Myrtus communis. Antimicrob Agents Chemother. 6(5):539–542
Salehi B, Krochmal-Marczak B, Skiba D, Patra JK, Das SK, Das G, Popović-Djordjević JB, Kostić AZ, Kumar NV, Tripathi A, Al-Snafi AE, Arserim-Uçar DK, Konovalov DA, Csupor D, Shukla I, Azmi L, Mishra AP, Sharifi-Rad J, Sawicka B, Martins N, Taheri Y, Fokou BVT, Capasso R, Martorell M (2019) Convolvulus plant—a comprehensive review from phytochemical composition to pharmacy. Phytother Res 34:315–328
Salehpour K, Abadi TM, Ghorbani MR (2016) Study effect of medicinal plant Myrtus communis on some blood biochemical parameters on Arabi sheep. Basrah J Veter Res 15(3):219–224
Samareh Fekri M, Mandegary A, Sharififar F, Poursalehi HR, Nematollahi MH, Izadi A, Mehdipour M, Asadi A, Samareh FM (2018) Protective effect of standardized extract of Myrtus communis L. (myrtle) on experimentally bleomycin-induced pulmonary fibrosis: biochemical and histopathological study. Drug Chem Toxicol. 41(4):408–414
Sen A, Ozkan S, Recebova K, Cevik O, Ercan F, Kervancıoglu Demirci E, Bitis L, Sener G (2016) Effects of Myrtus communis extract treatment in bile duct ligated rats. J Surg Res. 205(2):359–367
Shahmohammadi Z, Sojoodi MM, Kamalinejad M, Faizi M (2012) Evaluation of sedative-hypnotic effect of Myrtus communis L. aqueous extract in mice. Res Pharm Sci. 7(5):S832
Shahnazi M, Azadmehr A, Jondabeh MD, Hajiaghaee R, Norian R, Aghaei H, Saraei M, Alipour M (2017) Evaluating the effect of Myrtus communis on programmed cell death in hydatid cyst protoscolices. Asian Pac J Trop Med. 10(11):1072–1076
Sidkey BA (2018) Antibacterial and antioxidant properties of Bougainvillea spectabilis L. and Myrtus communis leaves extracts. GJBB. 7(3):336–342
Sisay M, Gashaw T (2017) Ethnobotanical, ethnopharmacological, and phytochemical studies of Myrtus communis Linn: a popular herb in unani system of medicine. J Evid Based Complementary Altern Med 22(4):1035–1043. https://doi.org/10.1177/2156587217718958
Sisay M, Engidawork E, Shibeshi W (2017) Evaluation of the antidiarrheal activity of the leaf extracts of Myrtus communis Linn (myrtaceae) in mice model. BMC Complement Altern Med 17(1):103
Soomro S, Mesaik MA, Shaheen F, Khan N, Halim SA, Ul-Haq Z, Ali Siddiqui R, Choudhary MI (2019) Inhibitory effects of myrtucommuacetalone 1 (MCA-1) from Myrtus communis on inflammatory response in mouse macrophages. Molecules. 25(1):E13
Sumbul S, Ahmad MA, Asif M, Saud I, Akhtar M (2010) Evaluation of Myrtus communis Linn. berries (common myrtle) in experimental ulcer models in rats. Hum Exp Toxicol. 29(11):935–944
Sumbul S, Ahmad MA, Asif M, Akhtar M, Saud I (2012) Physicochemical and phytochemical standardization of berries of Myrtus communis Linn. J Pharm Bioallied Sci. 4(4):322–326
Taamalli A, Iswaldi I, Arráez-Román D, Segura-Carretero A, Fernández-Gutiérrez A, Zarrouk M (2014) UPLC-QTOF/MS for a rapid characterisation of phenolic compounds from leaves of Myrtus communis L. Phytochem Anal. 25(1):89–96
Taamalli A, Iswaldi I, Arraez-Roman D, Segura-Carretero A, Fernandez-Gutierrez A, Zarrouk M (2014) UPLC−QTOF/MSfor a rapid characterisation of phenolic compounds from leaves of Myrtus communis L. phytochem. Anal. 2014(25):89–96
Taamalli A, Iswaldi I, Arraez-Roman D, Segura-Carretero A, Fernadez-Gutierrez A, Zarrouk M (2014) UPLC-QTQF/MS for a rapid characterization of phenolic compounds from leaves of Myrtus communis L. phytochem. Anal. 2014(25):89–96
Tas S, Tas B, Bassalat N, Jaradat N (2018) In vivo, hypoglycemic, hypolipidemic and oxidative stress inhibitory activities of Myrtus communis L. fruits hydroalcoholic extract in normoglycemic and streptozotocin-induced diabetic rats. Biomed Res 29(13):2727–2734
Tayoub G, Alnaser AA, Ghanem I (2012) Fumigant activity of leaf essential oil from Myrtus communis L. against the khapra beetle. Int J Med Arom Plants 2(1):207–213
Teibo JO, Ayinde KS, Olaoba OT, Adelusi TI, Teibo TK, Bamikunle MV, Batiha GES (2021) Functional foods’ bioactive components and their chemoprevention mechanism in cervical, breast, and liver cancers: a systematic review. Funct FoodsHealth Dis. 11:559–585
Teimoory H, Azizi M, Najafi MF, Behzadi A, Rezaei M (2013) Antibacterial activity of Myrtus communis L. and Zingiber officinale rose extracts against some gram positive pathogens. Res Opin Anim Vet Sci. 3(12):478–481
Tretiakova I, Blaesius D, Maxia L, Wesselborg S, Schulze-Osthoff K, Cinatl J, Michaelis M, Werz O (2008) Myrtucommulone from Myrtus communis induces apoptosis in cancer cells via the mitochondrial pathway involving caspase-9. Apoptosis. 13(1):119–131
Tuberoso CIG, Rosa A, Bifulco E, Melis MP, Atzeri A, Pirisi FM, Dessì MA (2010) Chemical composition and antioxidant activities of Myrtus communis L. berries extracts. Food Chem. 123:1242–1250
Tumen I, Senol FS, Orhan IE (2012) Inhibitory potential of the leaves and berries of Myrtus communis L. (myrtle) against enzymes linked to neurodegenerative diseases and their antioxidant actions. Int J Food Sci Nutr 63(4):387–392
U.S. National Plant Germplasm System, Myrtus communis, https://npgsweb.ars-grin.gov/gringlobal/taxonomydetail.aspx?id=24898
Wannes WA, Marzouk B (2013) Differences between myrtle fruit parts (Myrtus communis var italica) in phenolics and antioxidant contents. J Food Biochem. 37:585–594
Wannes WA, Marzouk B (2016) Characterization of myrtle seed (Myrtus communis var. baetica) as a source of lipids, phenolics, and antioxidant activities. J Food Drug Anal. 24(2):316–323
Yadegarinia D, Gachkar L, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I (2006) Biochemical activities of iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry. 67(12):1249–1255
Yoshimura M, Amakura Y, Tokuhara M, Yoshida T (2008) Polyphenolic compounds isolated from the leaves of Myrtus communis. J Nat Med. 62(3):366–368
Youness ER, Mohamed NA, Ashour MN, Aly HF, Ibrahim AMM (2016) Neuroprotective effect of Myrtus communis and Zingbar officinale in LPS induced neurotoxicity in brain rats’ model. Der PharmaChemica. 8(19):474–482
Zohalinezhad ME, Hosseini-Asl MK, Akrami R, Nimrouzi M, Salehi A, Zarshenas MM (2016) Myrtus communis L. freeze-dried aqueous extract versus omeprazol in gastrointestinal reflux disease: a double-blind randomized controlled clinical trial. J Evid Based Complementary Altern Med. 21(1):23–29
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Al-Snafi, A.E., Teibo, J.O., Shaheen, H.M. et al. The therapeutic value of Myrtus communis L.: an updated review. Naunyn-Schmiedeberg's Arch Pharmacol 397, 4579–4600 (2024). https://doi.org/10.1007/s00210-024-02958-3
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DOI: https://doi.org/10.1007/s00210-024-02958-3