Abstract
Gastric ulcer is one of the most frequent gastrointestinal ailments worldwide. Indomethacin, one of the most potent NSAIDs, suffers undesirable ulcerogenic activity. Caffeic acid phenethyl ester (CAPE) has known health benefits. The current study examined the potential of CAPE to combat indomethacin-induced gastric ulcers in rats. Animals were randomized into 5 groups: control, Indomethacin (50 mg/kg) mg/kg), Indomethacin + CAPE (5 mg/kg/day), Indomethacin + CAPE (10 mg/kg), and Indomethacin + Omeprazole (30 mg/kg). CAPE prevented the rise in ulcer index, attenuated histopathological changes and preserved gastric mucin concentration. CAPE efficiently significantly prevented accumulation of malondialdehude (MDA) and prevented exhaustion of the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD). Further, CAPE prevented the rise in the expression of tumor necrosis factor-α (TNF-α), cyclo-oxygenase-2 (COX-2) and nuclear factor kapp-B (NFκB). This was associated with down-regulation of Bax and up-regulation of Bcl-2 mRNA. Finally, CAPE prevented induced indomethacin-induced decrease in heat shock protein 70 (HSP70) in gastric tissues. In conclusion, CAPE possesses the ability to prevent indomethacin-induced gastric ulcer in rats. This involves, at least partially, antioxidation, anti-inflammation, anti-apoptosis and enhancement of HSP70 expression.
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Acknowledgements
This project was funded by the Institutional Fund Projects under grant no. (IFPRC-150-166-2020). Therefore, the author gratefully acknowledges the technical and financial support from the Ministry of Education and King Abdulaziz University, Jeddah, Saudi Arabia. In addition, I am grateful to Gamal Abdel Aziz of the Department of Anatomy and Histology, Faculty of Medicine, King Abdulaziz University for his help in the histological studies.
Funding
This project was funded by the Institutional Fund Projects under grant no. (IFPRC-150–166-2020). Therefore, the author gratefully acknowledges the technical and financial support from the Ministry of Education and King Abdulaziz University, Jeddah, Saudi Arabia.
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Thikryat Neamatallah is responsible for the conceptualization, methodology, formal analysis, data curation, writing (original draft preparation), writing (review, editing), and project administration. The authors declare that all data were generated in-house and that no paper mill was used.
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Neamatallah, T. Caffeic acid phenethyl ester attenuates indomethacin-induced gastric ulcer in rats. Naunyn-Schmiedeberg's Arch Pharmacol 397, 1791–1801 (2024). https://doi.org/10.1007/s00210-023-02730-z
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DOI: https://doi.org/10.1007/s00210-023-02730-z