Abstract
Treatment with the chemotherapeutic agent, doxorubicin (DOX), is limited by nephrotoxicity. We investigated the possible protective effect of infliximab, a tumor necrosis factor alpha (TNF-α) inhibitor on DOX-induced nephrotoxicity. Rats were treated with a single intraperitoneal (ip) injection of DOX (17.5 mg/kg) in the absence or presence of infliximab (5 mg/kg, i.p.). Plasma and urinary markers of kidney function, oxidative stress, and inflammation were measured. Kidney and heart tissue was evaluated histopathologically. DOX-induced nephrotoxicity was confirmed by increased plasma urea, creatinine, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL), and clusterin concentrations. In addition, DOX increased urinary albumin/creatinine ratio, N-acetyl-β-D-glucosaminidase (NAG) activity, kidney injury molecule (KIM-1) concentrations, and reduced creatinine clearance. DOX significantly reduced renal antioxidants and increased plasma inflammatory markers and adiponectin concentrations. Concomitant treatment with infliximab did not significantly affect DOX-induced changes in plasma creatinine, cystatin C, or creatinine clearance. However, infliximab significantly reduced DOX-induced action on plasma urea, NGAL, clusterin, and adiponectin. Infliximab also significantly reduced urinary albumin/creatinine ratio, NAG activity, and KIM-1 concentrations, as well as the occurrence of fibrotic lesions in kidney tissue. Fibrosis detected in the heart was unchanged. In addition, infliximab reduced DOX-induced effects on plasma inflammatory markers, renal superoxide dismutase (SOD) and total antioxidant capacity. Our results show that infliximab is partially effective in mitigating DOX-induced nephrotoxicity in rats.
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Abdelrahman AM, Al Suleimani YM, Ashique M, Manoj P, Ali BH (2018) Effect of infliximab and tocilizumab on fructose-induced hyperinsulinemia and hypertension in rats. Biomed Pharmacother 105:182–186
Ali BH, Alza'abi M, Ramkumar A, Al-Lawati I, Waly MI, Beegam S, Nemmar A, Brand S, Schupp N (2014) The effect of activated charcoal on adenine-induced chronic renal failure in rats. Food Chem Toxicol 65:321–328
Cianciolo R, Yoon L, Krull D, Stokes A, Rodriguez A, Jordan H, Cooper D, Falls JG, Cullen J, Kimbrough C, Berridge B (2013) Gene expression analysis and urinary biomarker assays reveal activation of tubulointerstitial injury pathways in a rodent model of chronic proteinuria (doxorubicin nephropathy). Nephron Exp Nephrol 124:1–10
Cure E, Kirbas A, Tumkaya L, Cure MC, Kalkan Y, Yilmaz A, Yuce S (2015) Protective effect of infliximab on methotrexate-induced liver injury in rats: unexpected drug interaction. J Cancer Res Ther 11:164–169
Devarajan P (2008) NGAL in acute kidney injury: from serendipity to utility. Am J Kidney Dis 52:395–399
el Nahas AM, Bassett AH, Cope GH, Le Carpentier JE (1991) Role of growth hormone in the development of experimental renal scarring. Kidney Int 40:29–34
El-Sayed EM, Mansour AM, El-Sawy WS (2017) Protective effect of proanthocyanidins against doxorubicin-induced nephrotoxicity in rats. J Biochem Mol Toxicol 31
El-Sheikh AA, Morsy MA, Mahmoud MM, Rifaai RA, Abdelrahman AM (2012) Effect of coenzyme-q10 on doxorubicin-induced nephrotoxicity in rats. Adv Pharmacol Sci 2012:981461
Guo J, Guan Q, Liu X, Wang H, Gleave ME, Nguan CY, Du C (2016) Relationship of clusterin with renal inflammation and fibrosis after the recovery phase of ischemia-reperfusion injury. BMC Nephrol 17:133
Ibrahim MA, Morsy MA, Hafez HM, Gomaa WM, Abdelrahman AM (2012) Effect of selective and non-selective cyclooxygenase inhibitors on doxorubicin-induced cardiotoxicity and nephrotoxicity in rats. Toxicol Mech Methods 22:424–431
Jin X, Chen J, Hu Z, Chan L, Wang Y (2013) Genetic deficiency of adiponectin protects against acute kidney injury. Kidney Int 83:604–614
Karson A, Demirtas T, Bayramgurler D, Balci F, Utkan T (2013) Chronic administration of infliximab (TNF-alpha inhibitor) decreases depression and anxiety-like behaviour in rat model of chronic mild stress. Basic Clin Pharmacol Toxicol 112:335–340
Khames A, Khalaf MM, Gad AM, Abd El-Raouf OM (2017) Ameliorative effects of sildenafil and/or febuxostat on doxorubicin-induced nephrotoxicity in rats. Eur J Pharmacol 805:118–124
Kirbas A, Cure MC, Kalkan Y, Cure E, Tumkaya L, Sahin OZ, Yuce S, Kizilkaya B, Pergel A (2015) Effect of infliximab on renal injury due to methotrexate in rat. Iran J Kidney Dis 9:221–229
Lee VW, Harris DC (2011) Adriamycin nephropathy: a model of focal segmental glomerulosclerosis. Nephrology (Carlton) 16:30–38
Lv W, Booz GW, Wang Y, Fan F, Roman RJ (2018) Inflammation and renal fibrosis: recent developments on key signaling molecules as potential therapeutic targets. Eur J Pharmacol 820:65–76
Mohan M, Kamble S, Gadhi P, Kasture S (2010) Protective effect of Solanum torvum on doxorubicin-induced nephrotoxicity in rats. Food Chem Toxicol 48:436–440
Moriwaki Y, Inokuchi T, Yamamoto A, Ka T, Tsutsumi Z, Takahashi S, Yamamoto T (2007) Effect of TNF-alpha inhibition on urinary albumin excretion in experimental diabetic rats. Acta Diabetol 44:215–218
Nagai K, Fukuno S, Otani K, Nagamine Y, Omotani S, Hatsuda Y, Myotoku M, Konishi H (2018) Prevention of doxorubicin-induced renal toxicity by Theanine in rats. Pharmacology 101:219–224
Oh J, Rabb H (2013) Adiponectin: an enlarging role in acute kidney injury. Kidney Int 83:546–548
Omote K, Gohda T, Murakoshi M, Sasaki Y, Kazuno S, Fujimura T, Ishizaka M, Sonoda Y, Tomino Y (2014) Role of the TNF pathway in the progression of diabetic nephropathy in KK-A(y) mice. Am J Physiol Renal Physiol 306:F1335–F1347
Rasband WS (1997-2008) ImageJ. U. S. National Institutes of Health Bethesda, Maryland, USA
Rashikh A, Pillai KK, Najmi AK (2014) Protective effect of a direct renin inhibitor in acute murine model of cardiotoxicity and nephrotoxicity. Fundam Clin Pharmacol 28:489–500
Refaie MM, Amin EF, El-Tahawy NF, Abdelrahman AM (2016) Possible protective effect of Diacerein on doxorubicin-induced nephrotoxicity in rats. J Toxicol 2016:9507563
Ren X, Bo Y, Fan J, Chen M, Xu D, Dong Y, He H, Ren X, Qu R, Jin Y, Zhao W, Xu C (2016) Dalbergioidin ameliorates doxorubicin-induced renal fibrosis by suppressing the TGF-beta signal pathway. Mediat Inflamm 2016:5147571
Saritemur M, Un H, Cadirci E, Karakus E, Akpinar E, Halici Z, Ugan RA, Karaman A, Atmaca HT (2015) Tnf-alpha inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy. Environ Toxicol Pharmacol 39:577–588
Szalay CI, Erdelyi K, Kokeny G, Lajtar E, Godo M, Revesz C, Kaucsar T, Kiss N, Sarkozy M, Csont T, Krenacs T, Szenasi G, Pacher P, Hamar P (2015) Oxidative/Nitrative stress and inflammation drive progression of doxorubicin-induced renal fibrosis in rats as revealed by comparing a Normal and a fibrosis-resistant rat strain. PLoS One 10:e0127090
Tasdemir C, Tasdemir S, Vardi N, Ates B, Parlakpinar H, Kati B, Karaaslan MG, Acet A (2012) Protective effect of infliximab on ischemia/reperfusion-induced damage in rat kidney. Ren Fail 34:1144–1149
Tian M, Tang L, Wu Y, Beddhu S, Huang Y (2018) Adiponectin attenuates kidney injury and fibrosis in deoxycorticosterone acetate-salt and angiotensin II-induced CKD mice. Am J Physiol Renal Physiol 315:F558–F571
Tulubas F, Gurel A, Oran M, Topcu B, Caglar V, Uygur E (2015) The protective effects of omega-3 fatty acids on doxorubicin-induced hepatotoxicity and nephrotoxicity in rats. Toxicol Ind Health 31:638–644
Zhang Y, Xu Y, Qi Y, Xu L, Song S, Yin L, Tao X, Zhen Y, Han X, Ma X, Liu K, Peng J (2017) Protective effects of dioscin against doxorubicin-induced nephrotoxicity via adjusting FXR-mediated oxidative stress and inflammation. Toxicology 378:53–64
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Supported by a grant from Sultan Qaboos University (IG/MED//PHAR/16/02).
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AA, YS, and BA designed research, participated in the interpretation of the results, wrote and reviewed the manuscript. NS performed the histological analysis, wrote and reviewed the manuscript. MA and PM conducted the experiments and analyzed. All authors read and approved the manuscript.
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Abdelrahman, A.M., Al Suleimani, Y.M., Manoj, P. et al. Effect of infliximab, a tumor necrosis factor-alpha inhibitor, on doxorubicin-induced nephrotoxicity in rats. Naunyn-Schmiedeberg's Arch Pharmacol 393, 121–130 (2020). https://doi.org/10.1007/s00210-019-01719-x
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DOI: https://doi.org/10.1007/s00210-019-01719-x