Abstract
Excessive alcohol consumption provides risk to cardiomyopathy with unknown mechanisms. Resveratrol, a plant polyphenol, is widely reported for its cardiovascular benefits, while its effect on alcohol-induced impairments in cardiomyocytes largely remains unknown. Effects of resveratrol on the cardiomyocytes under ethanol insult were studied in vitro. Ethanol exposure in mouse neonatal cardiomyocytes increased cell death and induced a specific loss of tight junction protein, connexin 43. In spite of adverse effects at higher concentrations, resveratrol at 10 μM improved cell viability of cardiomyocytes in the presence of a deleterious dose of ethanol. Importantly, the co-treatment of resveratrol with ethanol exhibited the restoration of connexin 43 protein. Further assays showed that these effects were likely associated with the antioxidative actions of resveratrol, and correlated with the alleviation of MAP kinase activation in cultured cardiomyocytes in response to ethanol. Our data suggests a novel mechanism of cardiomyocyte cell loss under ethanol exposure and provides new evidence of protective effects of resveratrol in the cardiomyocytes.
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The procedure regarding mouse handling and dissection was performed in accordance with the animal protocol approved by the Committee on the Ethics of Animal Experiments of Wuxi No. 2 People’s Hospital Affiliated to Nanjing Medical University.
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This study was supported by Science and Technology Development Fund of Nanjing Medical University (2014NJMU2D 037).
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The authors declare that there is no conflict of interests.
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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Tu, S., Cao, Ft., Fan, Xc. et al. Resveratrol protects the loss of connexin 43 induced by ethanol exposure in neonatal mouse cardiomyocytes. Naunyn-Schmiedeberg's Arch Pharmacol 390, 651–660 (2017). https://doi.org/10.1007/s00210-017-1368-1
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DOI: https://doi.org/10.1007/s00210-017-1368-1