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Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: wound healing and anti-inflammatory effects

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Abstract

Here, we evaluate the anti-inflammatory and wound-healing effects of methanolic crude extract obtained from aerial parts (leaves and branches) of Rubus imperialis Chum. Schl. (Rosaceae) and the pure compound niga-ichigoside F1. Anti-inflammatory activity was determined in vivo and in vitro, and the healing effect was evaluated in surgical lesions in mice skin. The 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and H2O2-induced oxidative stress were used to determine antioxidant activity. The efferocytosis activity was also determined. The data obtained show that the extract of R. imperialis promote reduction in the inflammatory process induced by lipopolysaccharide (LPS) or carrageenan in the air pouch model; the effects could be reinforced by nitric oxide reduction in LPS-stimulated neutrophils, and an increase in the efferocytosis. The extract showed wound healing property in vitro and in vivo, scavenging activity for DPPH, and cytoprotection in the H2O2-induced oxidative stress in L929 cells. In addition, the compound niga-ichigoside F1 was able to reduce the NO secretion; however, it did not present wound-healing activity in vitro. Together, the data obtained point out the modulatory actions of R. imperialis extract on leukocyte migration to the inflamed tissue, the antioxidant, and the pro-resolutive activity. However, the R. imperialis anti-inflammatory activity may be mediated in parts by niga-ichigoside F1, and on wound healing do not correlated with niga-ichigoside F1.

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Acknowledgments

The authors are grateful to the Conselho Nacional de Pesquisa e Tecnologia (CNPq, process n. 444682/2014-7), UNIVALI, and FAPESC for their financial support.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas and Núcleo de Investigações Químico-Farmacêuticas-NIQFAR, Universidade do Vale do Itajaí - UNIVALI, Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil

    Talita Dacroce Tonin, Liliani Carolini Thiesen, Maria Luisa de Oliveira Nunes, Milena Fronza Broering, Marcos Paulo Donato, Marina Jagielski Goss, Marcel Petreanu, Rivaldo Niero, Isabel Daufenback Machado & José Roberto Santin

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  1. Talita Dacroce Tonin
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  2. Liliani Carolini Thiesen
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  3. Maria Luisa de Oliveira Nunes
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Correspondence to José Roberto Santin.

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Tonin, T.D., Thiesen, L.C., de Oliveira Nunes, M.L. et al. Rubus imperialis (Rosaceae) extract and pure compound niga-ichigoside F1: wound healing and anti-inflammatory effects. Naunyn-Schmiedeberg's Arch Pharmacol 389, 1235–1244 (2016). https://doi.org/10.1007/s00210-016-1285-8

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