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Cloricromene, a coumarine derivative, reduced the development of periodontitis in rats

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Abstract

Recent studies have demonstrated that cloricromene, a coumarin derivative, exerts protective effects in models of inflammation and shock. Tumour necrosis factor plays a pivotal role in the induction of genes involved in physiological processes, as well as in the response to inflammation. We investigated the effect of cloricromene in a rat model of periodontitis. Periodontitis was induced in rats by placing a 2/0 braided silk ligature around the lower left first molar. At day 8 the gingivomucosal tissue encircling the mandibular first molar was removed for evaluation of tumour necrosis factor production, neutrophil infiltration, tissue permeability, nitrotyrosine formation, poly (ADP-ribose) polymerase activation, radiography and histology. Ligation significantly induced an increased tumour necrosis factor production, neutrophil infiltration and a positive staining for nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone erosion as evaluated by radiography analysis. Intraperitonal injection of cloricromene (10 mg/kg daily for 8 days) significantly decreased all of the parameters of inflammation as described above. This suggests that cloricromene treatment, which reduced tumour necrosis factor production, may be of benefit in the treatment of periodontitis.

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Acknowledgements

This study was supported by grant PRA 2003 from the University of Messina. The authors would like to thank Di Paolo Lelio and Giovanni Pergolizzi for their excellent technical assistance during this study, Mrs Caterina Cutrona for secretarial assistance and Miss Valentina Malvagni for editorial assistance with the manuscript.

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Authors and Affiliations

  1. Department of Clinical and Experimental Medicine and Pharmacology, Torre Biologica, Policlinico Universitario, 98123, Messina, Italy

    Carmelo Muià, Emanuela Mazzon, Daniele Zito, Daniele Maiere, Concetta Crisafulli & Salvatore Cuzzocrea

  2. Department of Clinical Veterinary Science, University of Teramo, Teramo, Italy

    Domenico Britti

  3. Istituto Policattedra di Odontostomatologia, Universita degli Studi di Messina, Messina, Italy

    Giacomo Oteri & Giancarlo Cordasco

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  1. Carmelo Muià
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  2. Emanuela Mazzon
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Correspondence to Salvatore Cuzzocrea.

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Muià, C., Mazzon, E., Zito, D. et al. Cloricromene, a coumarine derivative, reduced the development of periodontitis in rats. Naunyn Schmied Arch Pharmacol 373, 51–59 (2006). https://doi.org/10.1007/s00210-006-0048-3

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