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Systemic administration of curcumin or piperine enhances the periodontal repair: a preliminary study in rats

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Abstract

Objectives

Studies have documented the anti-inflammatory effects of spices, which may be related to treatment of chronic diseases. The purpose of this study was to evaluate the influence of curcumin and piperine and their association on experimental periodontal repair in rats.

Materials and methods

Periodontitis was induced via the installation of a ligature around the first molar. After 15 days, the ligatures were removed, and the rats were separated into groups (12 animals per group): (i) curcumin, (ii) piperine, (iii) curcumin+piperine, (iv) corn oil vehicle, and (v) control group (animals had ligature-induced periodontitis but were not treated). The compounds were administered daily, for 15 days by oral gavage. Animals were euthanized at 5 and 15 days, and hemimaxillae and gingival tissues were harvested. Bone repair was assessed by μCT (microcomputer tomography). Histological sections were stained with hematoxylin/eosin (H/E) for the assessment of cellular infiltrate or picrosirius red for quantification of collagen content, and subjected to immunohistochemistry for detecting NF-ĸB. Gingival tissues were used to evaluate levels of TGF-β and IL-10 (ELISA).

Results

Curcumin and piperine increased the TGF-β level, significantly improved the collagen repair, and decreased the cellularity and activation of NF-ĸB in the periodontal tissues, but only curcumin caused a significant increase in early bone repair.

Conclusion

Curcumin and piperine promoted a substantive effect on tissue repair; however, there was not synergistic effect of compounds administered in combination.

Clinical relevance

Curcumin and piperine stimulates the tissue repair and may be potential candidates for the treatment of periodontal disease.

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Funding

The work was supported by FAPESP (São Paulo Research Foundation) 2010/19660-2 and 2010/20091-2.

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Authors and Affiliations

  1. Department of Diagnosis and Surgery, School of Dentistry at Araraquara - Univ Estadual Paulista (UNESP), Rua Humaita, 1680 – Centro, Araraquara, SP, 14801-903, Brazil

    Morgana R. Guimaraes-Stabili, Fabiana de Almeida Curylofo, Camilla Olga Tasso, Fernanda Regina Godoy Rocha, Marcell Costa de Medeiros & Carlos Rossa Jr

  2. Department of Clinical and Social Dentistry- Health Science Center, Federal University of Paraíba, João Pessoa, Brazil

    Sabrina Garcia de Aquino

  3. Department of Histology and Embriology, School of Dentistry at Araraquara, UNESP, Araraquara, SP, Brazil

    José Paulo de Pizzol Jr & Paulo Sérgio Cerri

  4. Department of Stomatology, School of Dentistry-University of Sao Paulo (USP), Sao Paulo, SP, Brazil

    Giuseppe Alexandre Romito

Authors
  1. Morgana R. Guimaraes-Stabili
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  2. Sabrina Garcia de Aquino
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  3. Fabiana de Almeida Curylofo
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  6. Marcell Costa de Medeiros
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  7. José Paulo de Pizzol Jr
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  8. Paulo Sérgio Cerri
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  9. Giuseppe Alexandre Romito
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  10. Carlos Rossa Jr
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Corresponding author

Correspondence to Morgana R. Guimaraes-Stabili.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All the experimental protocols were approved by the Ethical Committee for Animal Use (CEUA) of the School of Dentistry at Araraquara – UNESP and performed in accordance with the guidelines from the Brazilian College for Animal Experimentation (COBEA). The reported data conforms to the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Guimaraes-Stabili, M.R., de Aquino, S.G., de Almeida Curylofo, F. et al. Systemic administration of curcumin or piperine enhances the periodontal repair: a preliminary study in rats. Clin Oral Invest 23, 3297–3306 (2019). https://doi.org/10.1007/s00784-018-2755-9

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  • DOI: https://doi.org/10.1007/s00784-018-2755-9

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