Abstract
A new system has been developed to determine enzyme activities of glutathione transferase θ (GSTT1-1) based on radiometric product detection resulting from the enzymic reaction of methyl chloride with 35S-labelled glutathione. In principle, the method is universally applicable for determination of glutathione transferase activities towards a multiplicity of substrates. The method distinguishes between erythocyte GSTT1-1 activities of human `non-conjugators', `low conjugators' and `high conjugators'. Application to cytosol preparations of livers and kidneys of male and female Fischer 344 and B6C3F1 mice reveals differential GSTT1-1 activities in hepatic and renal tissues. These ought to be considered in species-specific modellings of organ toxicities of chlorinated hydrocarbons.
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Received: 3 September 1998 / Accepted: 29 September 1988
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Thier, R., Delbanco, E., Wiebel, F. et al. Determination of glutathione transferase (GSTT1-1) activities in different tissues based on formation of radioactive metabolites using 35S-glutathione. Arch Toxicol 72, 811–815 (1998). https://doi.org/10.1007/s002040050578
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DOI: https://doi.org/10.1007/s002040050578