Abstract
On the basis of an already established suspension organ culture system of mouse palate anlagen, we developed a corresponding culture system for rat palate anlagen. In order to optimize the culture results we systematically studied the influence of main “culture conditions” such as dissection technique, rotation speed, gassing schedule, and developmental stage at the onset of culture for mice and rat palate anlagen. This system allows culturing rat palate anlagen from day 15 of gestation to day 18+8 h (80 h) under serum- and antibiotic-free conditions using a chemically defined medium, resulting in 90% fused palates. The explants, containing the maxillary vault and the palatal shelves, were cultured in siliconized culture flasks at a rotation speed of 12 rpm and a temperature of 37°C (Table 1).
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References
Babiarz B, Wee EL, Zimmerman EF (1979) Palate morphogenesis. III. Changes in cell shape and orientation during shelf elevation. Teratology 20: 249–278
Barrach HJ, Neubert D (1980) Significance of organ culture techniques for evaluation of prenatal toxicity. Arch Toxicol 45: 161–187
Biggers JD, Gwatkin RBL, Heynes S (1961) Growth of embryonic avian and mammalian tibiae on a relatively simple chemically defined medium. Exp Cell Res 25: 41–58
Birch JR, Pirt SJ (1970) Improvements in a chemically defined medium for the growth of mouse cells (strain LS) in suspension. J Cell Sci 7: 661–670
Brinkley L (1980) In vitro studies of palatal shelf elevation. In: Pratt RM, Christiansen RL (eds) Current research trends in craniofacial development. Elsevier, North-Holland, pp 203–220
Brinkley L (1984) Changes in cell distribution during mouse secondary palate closure in vivo and in vitro. I. Epithelial cells. Dev Biol 102: 216–227
Brinkley L, Morris-Wiman J (1984) The role of extracellular matrices in palatal shelf closure. In: Moscona AA, Monroy A (eds.) Current topics in developmental biology. Academic Press, pp 17–36
Brinkley L, Basehoar G, Branch A, Avery A (1975) A new in vitro system for studying secondary palate development. J Embryol Exp Morphol 34: 485–495
Brinkley L, Basehoar G, Avery J (1978) Effects of craniofacial structures on mouse palatal closure in vitro. J Dent Res 57[2]: 402–411
Comper WD, Laurent TC (1978) Physiological function of connective tissue polysaccharides. Physiol Rev 58: 255–315
Diewert VM (1974) A cephalometric study of orofacial structures during secondary palate closure in the rat. Arch Oral Biol 19: 303–314
Diewert VM (1976) Graphic reconstructions of craniofacial structures during secondary palate development in rats. Teratology 14: 291–314
Diewert VM (1979) Experimental induction of premature movement of rat palatal shelves in vivo. J Anat 129: 597–601
Diewert VM (1980) The role of craniofacial growth in palatal shelf elevation. In: Pratt RM, Christiansen RL (eds) Current research trends in craniofacial development. Elsevier, North-Holland, pp 165–186
Ferguson MWJ (1977) The mechanisms of palatal shelf elevation and the pathogenesis of cleft palate. Virchows Arch Pathol Anat Histol 375: 97–113
Ferguson MWJ (1978) Palatal shelf elevation in the Wistar rat fetus. J Anat 125: 555–577
Goss AN, Bodner JW, Avery JK (1970a) In vitro fusion of cleft palate shelves. Cleft Palate J 7: 737–747
Goss AN, Bodner JW, Avery JK (1970b) Fusion capability of rat embryonic oral tissue in vitro. Arch Oral Biol 15: 797–804
Hassell JR, Orkin RW (1976) Synthesis and distribution of collagen in the rat palate during shelf elevation. Dev Biol 49: 80–88
Hudson CD, Shapiro BL (1973) A radioautographic study of DNA synthesis in embryonic rat palatal shelf epithelium with reference to the concept of programmed cell death. Arch Oral Biol 18: 77–84
Lahti A, Antila A, Saxen I (1972) The effect of hydrocortisone on the closure of the palatal shelves in two inbred strains of mice in vitro and in vivo. Teratology 9: 37–42
Long SY, Karsson KS, Lohmander S (1973) Cell proliferation in the cranial base of A/J mice with 6-AN-induced cleft palate. Teratology 8: 137–138
Merchant DJ, Hellmann KB, Schneider H, Muirhead EE (1962) Protection of animal cells with methylcellulose. Bacteriol Proc 1962: 141
Moriarty T, Weinstein S, Gibson R (1963) The development in vitro and in vivo of fusion of the palatal processes of rat embryos. J Embryol Exp Morphol 11: 605–619
Myers GS, Petrakis NL, Lee M (1967) Short communication: Cultivation of embryonic rat palate in defined and semi-defined media. Arch Oral Biol 12: 565–567
Neubert D, Merker HJ, Tapken S (1974) Comparative studies on the prenatal development of mosue extremities in vivo and in organ culture. Arch Pharmacol 286: 251–270
Pourtois M (1966) Onset of the acquired potentiality for fusion in the palatal shelves of rats. J Embryol Exp Morphol 16: 171–182
Pourtois M (1972) Morphogenesis of the primary and secondary palate. In: Slavkin HC, Bavetta S (eds) Developmental aspects of oral biology. Academic Press, New York, pp 81–108
Pratt RM, Goggins JF, Wilk AL, King CTG (1973) Acid mucopolysaccharide synthesis in the secondary palate of the developing rat at the time of rotation and fusion. Dev Biol 32: 230–237
Reeve W, Porter K, Levkovitz W (1966) In vitro closure of the rat palate. J Dent Res 45: 1375–1380
Ross LM, Walker BE (1967) Movement in palatine shelves in untreated and teratogen-treated mouse embryos. Am J Anat 121: 509–521
Shiota K, Kosazuma T, Klug S, Neubert D (1990) Development of the fetal mouse palate in suspension organ culture. Acta Anat 137: 59–64
Smiley GR, Koch WE (1975) A comparison of secondary palate development with different in vitro techniques. Anat Rec 181: 711–724
Thompson J, Schweisthal M (1969) Study of the closure of the embryonic rat palate in vitro with the effect of certain chemicals. J Dent Res 48: 568–572
Verrusio A (1970) A mechanism for closure of the secondary palate. Teratology 3: 17–20
Walker BE, Fraser FC (1956) Closure of the secondary palate in three strains of mice. J Embryol Exp Morphol 4: 176–189
Walker BE, Patterson A (1978) Palate development after fetal tongue removal in cortisone-treated mice. Teratology 17: 51–56
Wragg LE, Diewert VM, Klein M (1972) Spatial relations in the oral cavity and the mechanism of secondary palate closure in the rat. Arch Oral Biol 17: 683–690
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Al-Obaidi, N., Kastner, U., Merker, H.J. et al. Development of a suspension organ culture of the fetal rat palate. Arch Toxicol 69, 472–479 (1995). https://doi.org/10.1007/s002040050201
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DOI: https://doi.org/10.1007/s002040050201