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Effect of acute and repeated exposure to low doses of hydrazine on hepatic microsomal enzymes and biochemical parameters in vivo

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Abstract

A single dose of hydrazine (3 mg·kg−1 i.p.) caused hepatic accumulation of triglycerides and depletion of ATP in rats after 9 h. Repeated exposure of rats to hydrazine (≊2.5 mg·kg−1 per day) for 10 days resulted in depletion of hepatic reduced glutathione (GSH) and triglycerides. Repeated exposure to hydrazine also caused a significant (time dependent) induction ofp-nitrophenol hydroxylase (NPH) activity together with changes in other hepatic microsomal enzymes. These included 7-pentoxyresorufinO-deethylase (PROD) and 7-ethoxyresorufin O-de ethylase (EROD) activity, total cytochrome P450, cytochrome b5 and cytochrome P450 reductase activity. Repeated exposure to lower levels of hydrazine (≊0.250 mg·kg−1 per day) caused no significant hepatic biochemical or microsomal changes after 5 or 10 days except for an increase in NPH activity (17%) and liver ATP (15%) after 5 days.

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Authors and Affiliations

  1. Toxicology Department, School of Pharmacy, University of London, Brunswick Square, WC1N 1AX, London, UK

    Andrew M. Jenner & John A. Timbrell

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  1. Andrew M. Jenner
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  2. John A. Timbrell
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Jenner, A.M., Timbrell, J.A. Effect of acute and repeated exposure to low doses of hydrazine on hepatic microsomal enzymes and biochemical parameters in vivo. Arch Toxicol 68, 240–245 (1994). https://doi.org/10.1007/s002040050063

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  • DOI: https://doi.org/10.1007/s002040050063

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