Abstract
Chronic exposure to inorganic arsenic (iAs), a common drinking water and food contaminant, has been associated with an increased risk of type 2 diabetes in population studies worldwide. Several mechanisms underlying the diabetogenic effects of iAs have been proposed through laboratory investigations. We have previously shown that exposure to arsenite (iAs(III)) or its methylated trivalent metabolites, methylarsonite (MAs(III)) and dimethylarsinite (DMAs(III)), inhibits glucose-stimulated insulin secretion (GSIS) in pancreatic islets, without significant effects on insulin expression or insulin content. The goal of the present study was to determine if iAs(III) and/or its metabolites inhibit Ca2+ influx, an essential mechanism that regulates the release of insulin from β cells in response to glucose. We found that in vitro exposures for 48 h to non-cytotoxic concentrations of iAs(III), MAs(III), and DMAs(III) impaired Ca2+ influx in isolated murine pancreatic islets stimulated with glucose. MAs(III) and DMAs(III) were more potent inhibitors of Ca2+ influx than iAs(III). These arsenicals also inhibited Ca2+ influx and GSIS in islets treated with depolarizing levels of potassium chloride in the absence of glucose. Treatment with Bay K8644, a Cav1.2 channel agonist, did not restore insulin secretion in arsenical-exposed islets. Tolbutamide, a KATP channel blocker, prevented inhibition of insulin secretion in MAs(III)- and DMAs(III)-exposed islets, but only marginally in islets exposed to iAs(III). Our findings suggest that iAs(III), MAs(III), and DMAs(III) inhibit glucose-stimulated Ca2+ influx in pancreatic islets, possibly by interfering with KATP and/or Cav1.2 channel function. Notably, the mechanisms underlying inhibition of GSIS by iAs(III) may differ from those of its trivalent methylated metabolites.
Similar content being viewed by others
References
Ashfield R, Gribble FM, Ashcroft SJ, Ashcroft FM (1999) Identification of the high-affinity tolbutamide site on the SUR1 subunit of the KATP channel. Diabetes 48:1341–1347
Atlas D (2001) Functional and physical coupling of voltage-sensitive calcium channels with exocytotic proteins: ramifications for the secretion mechanism. J Neurochem 77:972–985
Babenko AP, Gonzalez G, Bryan J (1999) The tolbutamide site of SUR1 and a mechanism for its functional coupling to KATP channel closure. FEBS Lett 459:367–376
Barg S, Renström E, Berggren PO, Bertorello A, Bokvist K, Braun M, Eliasson L, Holmes WE, Köhler M, Rorsman P et al (1999) The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein. Proc Natl Acad Sci USA 96:5539–5544
Barg S, Ma X, Eliasson L, Galvanovskis J, Göpel SO, Obermüller S, Platzer J, Renström E, Trus M, Atlas D et al (2001) Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells. Biophys J 81:3308–3323
Catalucci D, Zhang DH, DeSantiago J et al (2009) Akt regulates L-type Ca2+ channel activity by modulating CaValpha1 protein stability. J Cell Biol 184(6):923–933
Chen L, Magliano DJ, Zimmet PZ (2012) The worldwide epidemiology of type 2 diabetes mellitus–present and future perspectives. Nat Rev Endocrinol 8:228–236
Cui X, Yang G, Pan M, Zhang X-N, Yang S-N (2012) Akt signals upstream of L-type calcium channels to optimize insulin secretion. Pancreas 41:15–21
Dadi PK, Vierra NC, Ustione A, Piston DW, Colbran RJ, Jacobson DA (2014) Inhibition of pancreatic β-Cell Ca2+/calmodulin-dependent protein kinase II reduces glucose-stimulated calcium influx and insulin secretion, impairing glucose tolerance. J Biol Chem 289:12435–12445
Del Razo LM, García-Vargas GG, Valenzuela OL, Castellanos EH, Sánchez-Peña LC, Currier JM, Drobná Z, Loomis D, Stýblo M (2011) Exposure to arsenic in drinking water is associated with increased prevalence of diabetes: a cross-sectional study in the Zimapán and Lagunera regions in Mexico. Environ Health 10:73
Díaz-Villaseñor A, Burns AL, Salazar AM, Sordo M, Hiriart M, Cebrián ME, Ostrosky-Wegman P (2008) Arsenite reduces insulin secretion in rat pancreatic beta-cells by decreasing the calcium-dependent calpain-10 proteolysis of SNAP-25. Toxicol Appl Pharmacol 231:291–299
Dolphin AC (2012) Calcium channel auxiliary α2δ and β subunits: trafficking and one step beyond. Nat Rev Neurosci 13:542–555
Douillet C, Currier J, Saunders J, Bodnar WM, Matousek T, Stýblo M (2013) Methylated trivalent arsenicals are potent inhibitors of glucose stimulated insulin secretion by murine pancreatic islets. Toxicol Appl Pharmacol 267:11–15
Dover EN, Beck R, Huang MC, Douillet C, Wang Z, Klett EL, Stýblo M (2017) Arsenite and methylarsonite inhibit mitochondrial metabolism and glucose-stimulated insulin secretion in INS-1 832/13 β cells. Arch, Toxicol
Eliasson L, Ma X, Renström E, Barg S, Berggren P-O, Galvanovskis J, Gromada J, Jing X, Lundquist I, Salehi A et al (2003) SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells. J Gen Physiol 121:181–197
Fu J, Woods CG, Yehuda-Shnaidman E, Zhang Q, Wong V, Collins S, Sun G, Andersen ME, Pi J (2010) Low-level arsenic impairs glucose-stimulated insulin secretion in pancreatic beta cells: involvement of cellular adaptive response to oxidative stress. Environ Health Perspect 118:864–870
Gao B, Sekido Y, Maximov A, Saad M, Forgacs E, Latif F, Wei MH, Lerman M, Lee JH, Perez-Reyes E et al (2000) Functional properties of a new voltage-dependent calcium channel alpha(2)delta auxiliary subunit gene (CACNA2D2). J Biol Chem 275:12237–12242
Gribble MO, Howard BV, Umans JG, Shara NM, Francesconi KA, Goessler W, Crainiceanu CM, Silbergeld EK, Guallar E, Navas-Acien A (2012) Arsenic exposure, diabetes prevalence, and diabetes control in the Strong Heart Study. Am J Epidemiol 176:865–874
Henquin JC (2000) Triggering and amplifying pathways of regulation of insulin secretion by glucose. Diabetes 49:1751–1760
Henquin JC (2009) Regulation of insulin secretion: a matter of phase control and amplitude modulation. Diabetologia 52:739
Izdebska M, Gagat M, Grzanka D, Grzanka A (2013) Ultrastructural localization of F-actin using phalloidin and quantum dots in HL-60 promyelocytic leukemia cell line after cell death induction by arsenic trioxide. Acta Histochem 115:487–495
Izdebska M, Klimaszewska-Wiśniewska A, Lewandowski D, Nowak JM, Gagat M, Grzanka A (2014) Arsenic trioxide preferentially induces nonapoptotic cell deaths as well as actin cytoskeleton rearrangement in the CHO AA8 cell line. Postępy Higieny i Medycyny Doświadczalnej 68:1492–1500
Kuo C-C, Moon KA, Wang S-L, Silbergeld E, Navas-Acien A (2017) The association of arsenic metabolism with cancer, cardiovascular disease, and diabetes: a systematic review of the epidemiological evidence. Environ Health Perspect 125:087001
Lau A, Whitman SA, Jaramillo MC, Zhang DD (2013) Arsenic-mediated activation of the Nrf2-Keap1 antioxidant pathway. J Biochem Mol Toxicol 27:99–105
Martin EM, Stýblo M, Fry RC (2017) Genetic and epigenetic mechanisms underlying arsenic-associated diabetes mellitus: a perspective of the current evidence. Epigenomics 9:701–710
Maull EA, Ahsan H, Edwards J, Longnecker MP, Navas-Acien A, Pi J, Silbergeld EK, Stýblo M, Tseng C-H, Thayer KA et al (2012) Evaluation of the association between arsenic and diabetes: a National Toxicology Program Workshop Review. Environ Health Perspect 120:1658–1670
Panten U, Zielmann S, Schrader M-T, Lenzen S (1985) The dihydropyridine derivative, Bay K 8644, enhances insulin secretion by isolated pancreatic islets. Naunyn-Schmiedeberg’s Arch Pharmacol 328:351–353
Paul DS, Harmon AW, Devesa V, Thomas DJ, Stýblo M (2007) Molecular mechanisms of the diabetogenic effects of arsenic: inhibition of insulin signaling by arsenite and methylarsonous acid. Environ Health Perspect 115:734–742
Peng Q, Harlow SD, Park SK (2015) Urinary arsenic and insulin resistance in US Adolescents. Int J Hyg Environ Health 218:407–413
Prentki M, Nolan CJ (2006) Islet beta cell failure in type 2 diabetes. J Clin. Invest 116:1802–1812
Qian Y, Liu KJ, Chen Y, Flynn DC, Castranova V, Shi X (2005) Cdc42 regulates arsenic-induced NADPH oxidase activation and cell migration through actin filament reorganization. J Biol Chem 280:3875–3884
Rhee SY, Hwang Y-C, Woo J, Chin SO, Chon S, Kim YS (2013) Arsenic exposure and prevalence of diabetes mellitus in Korean Adults. J Korean Med Sci 28:861–868
Santulli G, Pagano G, Sardu C, Xie W, Reiken S, D’Ascia SL, Cannone M, Marziliano N, Trimarco B, Guise TA et al (2015) Calcium release channel RyR2 regulates insulin release and glucose homeostasis. J Clin Invest 125:1968–1978
Stýblo M, Drobná Z, Jaspers I, Lin S, Thomas DJ (2002) The role of biomethylation in toxicity and carcinogenicity of arsenic: a research update. Environ Health Perspect 110:767–771
Thayer KA, Heindel JJ, Bucher JR, Gallo MA (2012) Role of environmental chemicals in diabetes and obesity: a National Toxicology Program Workshop Review. Environ Health Perspect 120:779–789
Viard P, Butcher AJ, Halet G et al (2004) PI3 K promotes voltage-dependent calcium channel trafficking to the plasma membrane. Nat Neurosci 7:939–994
Wang Z, Thurmond DC (2009) Mechanisms of biphasic insulin-granule exocytosis—roles of the cytoskeleton, small GTPases and SNARE proteins. J Cell Sci 122:893–903
Wiser O, Bennett MK, Atlas D (1996) Functional interaction of syntaxin and SNAP-25 with voltage sensitive L- and N-type Ca2 + channels. EMBO J 15:4100–4110
Wiser O, Trus M, Hernández A, Renström E, Barg S, Rorsman P, Atlas D (1999) The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery. Proc Natl Acad Sci USA 96:248–253
Wollheim CB, Sharp GW (1981) Regulation of insulin release by calcium. Physiol Rev 61:914–973
World Health Organization (2016) Global report on diabetes. WHO Library Cataloguing-in-Publication Data. http://apps.who.int/iris/bitstream/10665/204871/1/9789241565257_eng.pdf
Yang S-N, Berggren P-O (2006) The role of voltage-gated calcium channels in pancreatic β-cell physiology and pathophysiology. Endocr Rev 27:621–676
Zhang C, Fennel EMJ, Douillet C, Stýblo M (2017) Exposures to arsenite and methylarsonite produce insulin resistance and impair insulin-dependent glycogen metabolism in hepatocytes. Arch Toxicol 91:3811–3821
Funding
This work was supported by a grant from the National Institutes of Environmental Health sciences [R01ES022697] to M.S., a National Research Service Award from the National Institute of Environmental Health Sciences [T32 ES007126] to M.H., and the UNC Nutrition Obesity Research Center funded by the National Institute of Diabetes and Digestive and Kidney Diseases [DK056350].
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflicts of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Huang, M., Douillet, C. & Stýblo, M. Arsenite and its trivalent methylated metabolites inhibit glucose-stimulated calcium influx and insulin secretion in murine pancreatic islets. Arch Toxicol 93, 2525–2533 (2019). https://doi.org/10.1007/s00204-019-02526-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00204-019-02526-2