Abstract
1,3-β-Glucan was a major cell wall component of fungus. The existing studies showed that 1,3-β-glucan exposure could induce lung inflammation that involved both Th1 and Th2 cytokines. Regulatory T cells (Treg cells) played a critical role in regulating immune homeostasis by adjusting the Th1/Th2 balance. The role of Treg cells and regulatory mechanism in 1,3-β-glucan-induced lung inflammation is still unclear. In our study, mice were exposed to 1,3-β-glucan by intratracheal instillation. To investigate the role of Treg cells in response to 1,3-β-glucan, we generated Treg-depleted mice by intraperitoneal administration of anti-CD25 mAb. The Treg-depleted mice showed more inflammatory cells and severer pathological inflammatory change in lung tissue. Depletion of Treg cells led to increased Th1 cytokines and decreased Th2 cytokines. Treg-depleted mice showed a decreased expression of anti-inflammation cytokine and lower-level expression of CTLA-4. In all, our study indicated that Treg cells participated in regulating the 1,3-β-glucan-induced lung inflammation. Depletion of Treg cells aggravated the 1,3-β-glucan-induced lung inflammation, regulated the Th1/Th2 balance by enhancing Th1 response. Treg cells exerted their modulation function depending on both direct and indirect mechanism during the 1,3-β-glucan-induced lung inflammation.
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Acknowledgments
We appreciate Professor Yongjun Jiang and Associate Professor Zining Zhang, key laboratory of AIDS Immunology of Ministry of Health, China Medical University, for their excellent help with the immunological knowledge and great technical support. And we thank Professor Yuhua Chen and Associate Professor Weidong Zhao, Department of Developmental Biology, China Medical University, for their professional guidance with the molecular biology experiments. This work was supported by a grant from the National Natural Science Foundation of China (No. 30771791).
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Liu, F., Weng, D., Chen, Y. et al. Depletion of CD4+CD25+Foxp3+ regulatory T cells with anti-CD25 antibody may exacerbate the 1,3-β-glucan-induced lung inflammatory response in mice. Arch Toxicol 85, 1383–1394 (2011). https://doi.org/10.1007/s00204-011-0673-6
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DOI: https://doi.org/10.1007/s00204-011-0673-6