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Fluoride-induced histopathology and synthesis of stress protein in liver and kidney of mice

  • Organ Toxicity and Mechanisms
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Abstract

Selective low (15 mg sodium fluoride (NaF)/L) and relatively high (150 mg NaF/L) doses of in vivo fluoride (F) treatment to Swiss albino mice through drinking water elicited organ-specific toxicological response. All the F-exposed groups showed severe alterations in both liver and kidney architectures, but there was no significant change in the rate of water consumption and body weight. Vacuolar degeneration, micronecrotic foci in the hepatocytes, and hepatocellular hypertrophy were evident in the mice exposed to low dose (15 mg NaF/L for 30 days) while sinusoidal dilation with enlarged central vein surrounded by deep-blue erythrocytes were preponderant when treated with the same dose for a period of 90 days. Blood filled spaces, disintegration of tubular epithelium, and atrophy of glomeruli were also recorded in the kidney of the same treatment group. Change in reduced glutathione level (GSH), glutathione-s-transferase (GST) activity, malondialdehyde (MDA) production in both liver and kidney, disturbances in liver function, induction of heat shock protein 70 (Hsp 70) expression in kidney and its down regulation in liver were positively correlated with histopathological lesion.

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Acknowledgments

Authors acknowledge Professor. S.K. Maitra for his help in analyzing the histological changes. Authors are grateful to Inter University Accelerator Centre (IUAC), New Delhi for the Project Fund (UFUP-40316) to AC and University Grants Commission (UGC), New Delhi for the Centre for Advanced Studies (CAS) grant to the Department of Zoology. SP is grateful for a CAS Project Fellowship, and SA is grateful to CSIR for the award of Senior Research Fellowship.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Radiation Genetics and Chemical Mutagenesis Laboratory, Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan, 731 235, India

    Ansuman Chattopadhyay & Santosh Podder

  2. Environmental Toxicology Laboratory, Department of Zoology, Centre for Advanced Studies, Visva-Bharati University, Santiniketan, 731 235, India

    Soumik Agarwal & Shelley Bhattacharya

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  1. Ansuman Chattopadhyay
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  2. Santosh Podder
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  3. Soumik Agarwal
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  4. Shelley Bhattacharya
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Correspondence to Ansuman Chattopadhyay.

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Chattopadhyay, A., Podder, S., Agarwal, S. et al. Fluoride-induced histopathology and synthesis of stress protein in liver and kidney of mice. Arch Toxicol 85, 327–335 (2011). https://doi.org/10.1007/s00204-010-0588-7

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  • DOI: https://doi.org/10.1007/s00204-010-0588-7

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