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Insulin-like growth factor-1 protects preimplantation embryos from anti-developmental actions of menadione

  • Reproductive Toxicology
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Abstract

Menadione is a naphthoquinone used as a vitamin K source in animal feed that can generate reactive oxygen species (ROS) and cause apoptosis. Here, we examined whether menadione reduces development of preimplantation bovine embryos in a ROS-dependent process and tested the hypothesis that actions of menadione would be reduced by insulin-like growth factor-1 (IGF-1). Menadione caused a concentration-dependent decrease in the proportion of embryos that became blastocysts. All concentrations tested (1, 2.5, and 5.0 μM) inhibited development. Treatment with 100 ng/ml IGF-1 reduced the magnitude of the anti-developmental effects of the two lowest menadione concentrations. Menadione also caused a concentration-dependent increase in the percent of cells positive for the TUNEL reaction. The response was lower for IGF-1-treated embryos. The effects of menadione were mediated by ROS because (1) the anti-developmental effect of menadione was blocked by the antioxidants dithiothreitol and Trolox and (2) menadione caused an increase in ROS generation. Treatment with IGF-1 did not reduce ROS formation in menadione-treated embryos. In conclusion, concentrations of menadione as low as 1.0 μM can compromise development of bovine preimplantation embryos to the blastocyst stage of development in a ROS-dependent mechanism. Anti-developmental actions of menadione can be blocked by IGF-1 through effects downstream of ROS generation.

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Acknowledgments

This project was supported by National Research Initiative Competitive Grant no. 2007-35203-18073 from the USDA Cooperative State Research, Education, and Extension Service. The authors thank William Rembert, for invaluable technical assistance, Marshall, Adam, and Alex Chernin and employees of Central Beef Packing Co. (Center Hill, FL) for providing ovaries; and Scott A. Randell of Southeastern Semen Services (Wellborn, FL) for donating semen.

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Authors and Affiliations

  1. Department of Animal Sciences, University of Florida, PO Box 110910, Gainesville, FL, 32611-0910, USA

    James I. Moss, Eduardo Pontes & Peter James Hansen

  2. Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil

    Eduardo Pontes

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  1. James I. Moss
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  2. Eduardo Pontes
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  3. Peter James Hansen
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Correspondence to Peter James Hansen.

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Moss, J.I., Pontes, E. & Hansen, P.J. Insulin-like growth factor-1 protects preimplantation embryos from anti-developmental actions of menadione. Arch Toxicol 83, 1001–1007 (2009). https://doi.org/10.1007/s00204-009-0458-3

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  • DOI: https://doi.org/10.1007/s00204-009-0458-3

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