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Modulation of doxorubicin-induced clastogenesis in Wistar rat bone marrow cells by vitamin B6

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Abstract

Vitamin B6 has shown to be a potentially effective antioxidant agent, and dietary antioxidants are also frequently valuable inhibitors of clastogenesis and carcinogenesis. The purpose of the present work was to study the clastogenicity of different doses of vitamin B6 and to examine the possible modulating effect of this vitamin on chromosomal damage induced by the antitumor agent doxorubicin in Wistar rats. Experimental groups were set up for pre- and simultaneous treatment with vitamin B6 alone or in combination with DXR. The data obtained from administering different doses of vitamin B6 (12.5–100 mg/kg b.w.) showed no significant increase in total chromosomal aberrations when compared with the negative control. The administration of two doses of 25 mg/kg b.w. or one dose of 50 mg/kg b.w. of vitamin B6 before doxorubicin injection seemed equally effective in protecting cells against doxorubicin clastogenicity. The anticlastogenic effect of vitamin B6 on DXR-induced chromosomal damage could be ascribed to its antioxidant properties. Vitamin B6 was not clastogenic or cytotoxic in rat bone marrow cells and it plays a role in inhibiting the clastogenicity induced by DXR.

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Abbreviations

Vit B6 :

Vitamin B6

DXR:

Doxorubicin

IM:

Mitotic index

NC:

Negative control

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Acknowledgments

The authors are grateful to Luiz Augusto da Costa Junior and Sueli Aparecida Neves for their technical assistance. This work was sponsored by FAPESP, CNPq and CAPES.

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Authors and Affiliations

  1. Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Paula Lumy Takeuchi & Catarina Satie Takahashi

  2. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Catarina Satie Takahashi

  3. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. Do Café, s/n, Ribeirão Preto, 14041-903, Brazil

    Lusânia Maria Greggi Antunes

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  1. Paula Lumy Takeuchi
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  2. Lusânia Maria Greggi Antunes
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  3. Catarina Satie Takahashi
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Correspondence to Lusânia Maria Greggi Antunes.

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Takeuchi, P.L., Antunes, L.M.G. & Takahashi, C.S. Modulation of doxorubicin-induced clastogenesis in Wistar rat bone marrow cells by vitamin B6 . Arch Toxicol 82, 869–873 (2008). https://doi.org/10.1007/s00204-008-0308-8

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  • DOI: https://doi.org/10.1007/s00204-008-0308-8

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