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Gene expression profiling in lung tissues from rats exposed to formaldehyde

  • Organ Toxicity and Mechanisms
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Abstract

Formaldehyde is a ubiquitous toxic organic compound recently classified as a carcinogen by the International Agency for Research on Cancer and one of the major factors causing sick building syndrome. In this study, we have investigated the effects of formaldehyde on mRNA expression in rat lung tissues by applying genomics. Rats were exposed to ambient air and two different concentrations of formaldehyde (0, 5, 10 ppm) for 2 weeks at 6 h/day and 5 days/week in an inhalation chamber. Malondialdehyde (MDA) assay and carbonyl spectrometric assay were conducted to determine lipid peroxidation and protein oxidation levels and Comet assays were used for genotoxicity evaluation. Level of MDA, carbonyl insertion and DNA damage in the lungs of rats exposed to FA were found to be dose dependently increased. Gene expression was evaluated by using a bio-array hybridization analysis. A total of 21 (2 up- and 19 down-regulated) genes were identified as biomarkers for formaldehyde effects. Several differentiated gene groups were found. Genes involved in apoptosis, immunity, metabolism, signal transduction, transportation, coagulation and oncogenesis were found to be up- and down-regulated. Among these genes, the mRNA expressions of cytochrome P450, hydroxymethylbilane synthase, glutathione reductase, carbonic anhydrase 2, natriuretic peptide receptor 3, lysosomal associated protein transmembrane 5, regulator of G-protein signaling 3, olfactomedin related ER localized protein, and poly (ADP-ribose) polymerase-1 were confirmed by quantitative RT-PCR analysis. In summary, the MDA lipid peroxidation and the carbonyl protein oxidation assays showed that cytotoxic effects increased with increasing formaldehyde levels. Genomic analysis showed that 21 genes were up- or down-regulated. Of these genes, nine were confirmed by quantitative RT-PCR and could be potential biomarkers for human diseases associated with formaldehyde exposure.

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Acknowledgments

This research was supported by the grant of Medical Research Center for Environmental Toxico-Genomics and Proteomics and funded by Korea Science and Engineering Foundations and ministry of Science and Technology and by the Ministry of Environment as “The Eco-Technopia 21 project”.

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Authors and Affiliations

  1. Graduate School of Medicine, Korea University, Anamdong 5 Ka, Sungbukku, Seoul, 136-701, South Korea

    Donggeun Sul, Eunha Oh, Sohee Phark, Eunkyung Cho & Seonyoung Choi

  2. MyGene Bioscience Institute, 202-16, Nonhyundong, Sungok Bldg. 5th floor, Kangnamku, Seoul, South Korea

    Hyunsook Kim & Woon-Won Jung

  3. School of Biological Sciences and Technology, Chonnam University, 300 Yongbongdong, Bukku, Kwangju, 500-757, South Korea

    Hyung-Sik Kang & Eun-Mi Kim

  4. Department of Immunology, College of Pharmacy, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul, 156-756, South Korea

    Kwang-Woo Hwang

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  1. Donggeun Sul
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  2. Hyunsook Kim
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  3. Eunha Oh
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  4. Sohee Phark
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  5. Eunkyung Cho
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  7. Hyung-Sik Kang
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  8. Eun-Mi Kim
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  10. Woon-Won Jung
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Correspondence to Donggeun Sul.

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Sul, D., Kim, H., Oh, E. et al. Gene expression profiling in lung tissues from rats exposed to formaldehyde. Arch Toxicol 81, 589–597 (2007). https://doi.org/10.1007/s00204-007-0182-9

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  • DOI: https://doi.org/10.1007/s00204-007-0182-9

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