Abstract
Alveolar macrophages (AM) have an important role in clearing particles from the lungs. In response to different stimuli they can release reactive oxygen species (ROS) and inflammatory mediators and promote pulmonary inflammation. We exposed rat AM to carbon black (CB) particles (0.63–20 μg/ml) and measured the generation of ROS by using the fluorescent probe 2′,7′-dichlorofluorescein diacetate. Fluorescence was elevated in a concentration dependent manner in the AM exposed to CB. Follow-up experiments using a series of enzyme inhibitors indicate that the ERK MAP kinase pathway and the p38 MAP kinase pathway may be involved in the formation of ROS.
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Acknowledgments
The authors acknowledge support by VISTA (The Norwegian Academy of Science and Letters/Statoil).
This investigation was carried out in accordance with the law and rules for experimental animals in Norway.
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Aam, B.B., Fonnum, F. Carbon black particles increase reactive oxygen species formation in rat alveolar macrophages in vitro. Arch Toxicol 81, 441–446 (2007). https://doi.org/10.1007/s00204-006-0164-3
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DOI: https://doi.org/10.1007/s00204-006-0164-3