Abstract
Quantum Dots (QDs) have emerged as versatile nanomaterials with origins spanning organic, inorganic, and natural sources, revolutionizing various biomedical applications, particularly in combating pathogenic biofilm formation. Biofilms, complex structures formed by microbial communities enveloped in exopolysaccharide matrices, pose formidable challenges to traditional antibiotics due to their high tolerance and resistance, exacerbating inefficacy issues in antibiotic treatments. QDs offer a promising solution, employing physical mechanisms like photothermal or photodynamic therapy to disrupt biofilms. Their efficacy is noteworthy, with lower susceptibility to resistance development and broad-spectrum action as compared to conventional antibiotic methods. The stability and durability of QDs ensure sustained biofilm activity, even in challenging environmental conditions. This comprehensive review delves into the synthesis, properties, and applications of Carbon Quantum Dots (CQDs), most widely used QDs, showcasing groundbreaking developments that position these nanomaterials at the forefront of cutting-edge research and innovation. These nanomaterials exhibit multifaceted mechanisms, disrupting cell walls and membranes, generating reactive oxygen species (ROS), and binding to nucleic materials, effectively inhibiting microbial proliferation. This opens transformative possibilities for healthcare interventions by providing insights into biofilm dynamics. However, challenges in size control necessitate ongoing research to refine fabrication techniques, ensure defect-free surfaces, and optimize biological activity. QDs emerge as microscopic yet potent tools, promising to contribute to a brighter future where quantum wonders shape innovative solutions to persistently challenging issues posed by pathogenic biofilms. Henceforth, this review aims to explore QDs as potential agents for inhibiting pathogenic microbial biofilms, elucidating the underlying mechanisms, addressing the current challenges, and highlighting their promising future potential.
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References
Abdel-Salam M, Omran B, Whitehead K, Baek K-H (2020) Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots. Molecules 25:4486. https://doi.org/10.3390/molecules25194486
Ashengroph M, Khaledi A, Bolbanabad EM (2020) Extracellular biosynthesis of cadmium sulphide quantum dot using cell-free extract of Pseudomonas chlororaphis CHR05 and its antibacterial activity. Process Biochem 89:63–70. https://doi.org/10.1016/j.procbio.2019.10.028
Białowąs W, Boudjemaa R, Steenkeste K et al (2024) Reactive oxygen species production by photoexcited (CuInS2)x(ZnS)1-x quantum dots and their phototoxicity towards Staphylococcus aureus bacteria. J Photochem Photobiol Chem 446:115165. https://doi.org/10.1016/j.jphotochem.2023.115165
Bing W, Sun H, Yan Z et al (2016) Programmed Bacteria Death Induced by Carbon dots with different surface charge. Small 12:4713–4718. https://doi.org/10.1002/smll.201600294
Boobalan T, Sethupathi M, Sengottuvelan N et al (2020) Mushroom-Derived Carbon Dots for Toxic Metal Ion Detection and as Antibacterial and Anticancer agents. ACS Appl Nano Mater 3:5910–5919. https://doi.org/10.1021/acsanm.0c01058
Brooks J, Lefebvre DD (2017) Optimization of conditions for cadmium selenide quantum dot biosynthesis in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 101:2735–2745. https://doi.org/10.1007/s00253-016-8056-9
Burlacchini G, Sandri A, Papetti A et al (2024) Evaluation of Antibacterial and Antibiofilm Activity of Rice Husk Extract against Staphylococcus aureus. Pathogens 13:80. https://doi.org/10.3390/pathogens13010080
Cao K, Chen M-M, Chang F-Y et al (2020) The biosynthesis of cadmium selenide quantum dots by Rhodotorula mucilaginosa PA-1 for photocatalysis. Biochem Eng J 156:107497. https://doi.org/10.1016/j.bej.2020.107497
Celebi O, Celebi D, Baser S et al (2024) Antibacterial activity of Boron compounds against Biofilm-forming pathogens. Biol Trace Elem Res 202:346–359. https://doi.org/10.1007/s12011-023-03768-z
Cui F, Ye Y, Ping J, Sun X (2020) Carbon dots: current advances in pathogenic bacteria monitoring and prospect applications. Biosens Bioelectron 156:112085. https://doi.org/10.1016/j.bios.2020.112085
Dassanayake RS, Wansapura PT, Tran P et al (2019) Cotton Cellulose-CdTe Quantum dots Composite films with inhibition of Biofilm-Forming S. Aureus. Fibers 7:57. https://doi.org/10.3390/fib7060057
Dong X, Overton CM, Tang Y et al (2021) Visible light-activated Carbon dots for inhibiting Biofilm formation and inactivating Biofilm-Associated bacterial cells. Front Bioeng Biotechnol 9:786077. https://doi.org/10.3389/fbioe.2021.786077
Dsouza FP, Dinesh S, Sharma S (2024) Understanding the intricacies of microbial biofilm formation and its endurance in chronic infections: a key to advancing biofilm-targeted therapeutic strategies. Arch Microbiol 206:85. https://doi.org/10.1007/s00203-023-03802-7
Dwitya SS, Hsueh Y-H, Wang SS-S, Lin K-S (2023) Ultrafine nitrogen-doped graphene quantum dot structure and antibacterial activities against Bacillus subtilis 3610. Mater Chem Phys 295:127135. https://doi.org/10.1016/j.matchemphys.2022.127135
Fawaz W, Hasian J, Alghoraibi I (2023) Synthesis and physicochemical characterization of carbon quantum dots produced from folic acid. Sci Rep 13:18641. https://doi.org/10.1038/s41598-023-46084-1
Gattu R, Ramesh SS, Ramesh S (2024) Role of small molecules and nanoparticles in effective inhibition of microbial biofilms: a ray of hope in combating microbial resistance. Microb Pathog 188:106543. https://doi.org/10.1016/j.micpath.2024.106543
Ghirardello M, Ramos-Soriano J, Galan MC (2021) Carbon dots as an Emergent Class of Antimicrobial agents. Nanomaterials 11:1877. https://doi.org/10.3390/nano11081877
Ghorbani M, Tajik H, Moradi M et al (2022) One-pot microbial approach to synthesize carbon dots from baker’s yeast-derived compounds for the preparation of antimicrobial membrane. J Environ Chem Eng 10:107525. https://doi.org/10.1016/j.jece.2022.107525
Giordano MG, Seganti G, Bartoli M, Tagliaferro A (2023) An overview on Carbon Quantum dots Optical and Chemical features. Molecules 28:2772. https://doi.org/10.3390/molecules28062772
Hussain S, Packirisamy G, Misra K et al (2022) Editorial: Quantum dots for Biological Applications. Front Bioeng Biotechnol 10:930213. https://doi.org/10.3389/fbioe.2022.930213
Jia Q-Y, Jia R, Chen C-M, Wang L (2023) Characterization of CdSe QDs biosynthesized by a recombinant Rhodopseudomonas palustris. Biochem Eng J 191:108771. https://doi.org/10.1016/j.bej.2022.108771
Jian H-J, Yu J, Li Y-J et al (2020) Highly adhesive carbon quantum dots from biogenic amines for prevention of biofilm formation. Chem Eng J 386:123913. https://doi.org/10.1016/j.cej.2019.123913
Kadyan P, Thillai Arasu P, Kataria SK (2024) Graphene Quantum dots: Green Synthesis, characterization, and antioxidant and antimicrobial potential. Int J Biomater 2024:1–11. https://doi.org/10.1155/2024/2626006
Kansay V, Sharma VD, Chandan G et al (2023) Sustainable synthesis of nitrogen-doped fluorescent carbon quantum dots derived from Cissus quadrangularis for biomarker applications. Mater Chem Phys 296:127237. https://doi.org/10.1016/j.matchemphys.2022.127237
Kumar VB, Lahav M, Gazit E (2024) Preventing biofilm formation and eradicating pathogenic bacteria by Zn doped histidine derived carbon quantum dots. J Mater Chem B 10.https://doi.org/10.1039/D3TB02488A. .
Lei Y, Zhu G, Dan J et al (2024) Synthesis, optical properties, cytotoxicity and aspergillus oryzae application of bio-synthesized ZnS quantum dots. Ceram Int S0272884224000488. https://doi.org/10.1016/j.ceramint.2024.01.048
Li P, Yu M, Ke X et al (2022) Cytocompatible Amphipathic Carbon Quantum Dots as Potent membrane-active Antibacterial agents with Low Drug Resistance and Effective Inhibition of Biofilm formation. ACS Appl Bio Mater 5:3290–3299. https://doi.org/10.1021/acsabm.2c00292
Liang G, Shi H, Qi Y et al (2020) Specific anti-biofilm activity of Carbon Quantum dots by destroying P. Gingivalis Biofilm related genes. Int J Nanomed Volume 15:5473–5489. https://doi.org/10.2147/IJN.S253416
Lin F, Li C, Chen Z (2018) Bacteria-derived Carbon dots inhibit biofilm formation of Escherichia coli without affecting cell growth. Front Microbiol 9:259. https://doi.org/10.3389/fmicb.2018.00259
Liu M, Huang L, Xu X et al (2022) Copper Doped Carbon dots for addressing bacterial biofilm formation, wound infection, and tooth staining. ACS Nano 16:9479–9497. https://doi.org/10.1021/acsnano.2c02518
Liu X, Yao H, Zhao X, Ge C (2023) Biofilm formation and control of Foodborne pathogenic Bacteria. Molecules 28:2432. https://doi.org/10.3390/molecules28062432
Lu F, Ma Y, Wang H et al (2021) Water-solvable carbon dots derived from curcumin and citric acid with enhanced broad-spectrum antibacterial and antibiofilm activity. Mater Today Commun 26:102000. https://doi.org/10.1016/j.mtcomm.2020.102000
Mareeswari P, Brijitta J, Harikrishna Etti S et al (2016) Rhizopus stolonifer mediated biosynthesis of biocompatible cadmium chalcogenide quantum dots. Enzyme Microb Technol 95:225–229. https://doi.org/10.1016/j.enzmictec.2016.08.016
Oliva-Arancibia B, Órdenes-Aenishanslins N, Bruna N et al (2017) Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440. J Biotechnol 264:29–37. https://doi.org/10.1016/j.jbiotec.2017.10.013
Ozyurt D, Kobaisi MA, Hocking RK, Fox B (2023) Properties, synthesis, and applications of carbon dots: a review. Carbon Trends 12:100276. https://doi.org/10.1016/j.cartre.2023.100276
Pang L, Lin H, Yang F, Deng D (2023) Editorial: mechanisms of biofilm development and antibiofilm strategies. Front Microbiol 14:1190611. https://doi.org/10.3389/fmicb.2023.1190611
Parambil AM, Prasad A, Tomar AK et al (2024) Biogenic carbon dots: a novel mechanistic approach to combat multidrug-resistant critical pathogens on the global priority list. J Mater Chem B 12:202–221. https://doi.org/10.1039/D3TB02374E
Priyadarshini E, Rawat K, Bohidar HB (2018) Quantum Dots-Based Nano-Coatings for Inhibition of Microbial Biofilms: A Mini Review. In: Stavrou VN (ed) Nonmagnetic and Magnetic Quantum Dots. InTech
Qin Z, Yue Q, Liang Y et al (2018) Extracellular biosynthesis of biocompatible cadmium sulfide quantum dots using Trametes Versicolor. J Biotechnol 284:52–56. https://doi.org/10.1016/j.jbiotec.2018.08.004
Rajendiran K, Zhao Z, Pei D-S, Fu A (2019) Antimicrobial activity and mechanism of Functionalized Quantum dots. Polymers 11:1670. https://doi.org/10.3390/polym11101670
Ran H-H, Cheng X, Bao Y-W et al (2019) Multifunctional quaternized carbon dots with enhanced biofilm penetration and eradication efficiencies. J Mater Chem B 7:5104–5114. https://doi.org/10.1039/C9TB00681H
Saadh MJ, Al-dolaimy F, Alamir HTA et al (2024) Emerging pathways in environmentally friendly synthesis of carbon-based quantum dots for exploring antibacterial resistance. Inorg Chem Commun 161:112012. https://doi.org/10.1016/j.inoche.2023.112012
Seth S, Karthikeyan, Rathinasabapathi P et al (2023) Quantum dots as antibacterial agents. Carbon and Graphene Quantum Dots for Biomedical Applications. Elsevier, pp 119–128
Sukmarini L, Atikana A, Hertiani T (2024) Antibiofilm activity of marine microbial natural products: potential peptide- and polyketide-derived molecules from marine microbes toward targeting biofilm-forming pathogens. J Nat Med 78:1–20. https://doi.org/10.1007/s11418-023-01754-2
Sun J, Liu F, Yu W et al (2021) Visualization of Vaccine Dynamics with Quantum dots for Immunotherapy. Angew Chem Int Ed 60:24275–24283. https://doi.org/10.1002/anie.202111093
Sun P, Xing Z, Li Z, Zhou W (2023) Recent advances in quantum dots photocatalysts. Chem Eng J 458:141399. https://doi.org/10.1016/j.cej.2023.141399
Tang S, Zhang H, Mei L et al (2022) Fucoidan-derived carbon dots against Enterococcus faecalis biofilm and infected dentinal tubules for the treatment of persistent endodontic infections. J Nanobiotechnol 20:321. https://doi.org/10.1186/s12951-022-01501-x
Tariq M, Singh A, Varshney N et al (2022) Biomass-derived carbon dots as an emergent antibacterial agent. Mater Today Commun 33:104347. https://doi.org/10.1016/j.mtcomm.2022.104347
Tenkayala NK, Katari NK, Gundla R et al (2024) Sustainable approach to synthesis of carbon Dot/ silver nanoparticles for biological evaluation as antimicrobial agent. Mater Res Express 11:015005. https://doi.org/10.1088/2053-1591/ad1128
Venegas FA, Saona LA, Monrás JP et al (2017) Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H 2 S release from cellular thiols. RSC Adv 7:40270–40278. https://doi.org/10.1039/C7RA03578K
Wang X, Feng Y, Dong P, Huang J (2019) A Mini Review on Carbon Quantum dots: Preparation, Properties, and Electrocatalytic Application. Front Chem 7:671. https://doi.org/10.3389/fchem.2019.00671
Wang H, Zhang M, Ma Y et al (2020) Selective inactivation of Gram-negative bacteria by carbon dots derived from natural biomass: Artemisia argyi leaves. J Mater Chem B 8:2666–2672. https://doi.org/10.1039/C9TB02735A
Wang W, Xu W, Zhang J et al (2024a) One-stop Integrated Nanoagent for Bacterial Biofilm Eradication and Wound Disinfection. ACS Nano 18:4089–4103. https://doi.org/10.1021/acsnano.3c08054
Wang X, Wang D, Lu H et al (2024b) Strategies to promote the Journey of Nanoparticles against Biofilm-Associated Infections. https://doi.org/10.1002/smll.202305988. Small 2305988
Wu Y, Van Der Mei HC, Busscher HJ, Ren Y (2020) Enhanced bacterial killing by Vancomycin in staphylococcal biofilms disrupted by novel, DMMA-modified carbon dots depends on EPS production. Colloids Surf B Biointerfaces 193:111114. https://doi.org/10.1016/j.colsurfb.2020.111114
Wu Y, Li C, Van Der Mei HC et al (2021a) Carbon Quantum dots Derived from different Carbon sources for Antibacterial Applications. Antibiotics 10:623. https://doi.org/10.3390/antibiotics10060623
Wu Z, Li X, Zhao Y et al (2021b) Organic Semiconductor/Carbon Dot Composites for Highly Efficient Hydrogen and Hydrogen Peroxide Coproduction from Water Photosplitting. ACS Appl Mater Interfaces 13:60561–60570. https://doi.org/10.1021/acsami.1c14735
Wu X, Abbas K, Yang Y et al (2022) Photodynamic Anti-bacteria by Carbon dots and their Nano-composites. Pharmaceuticals 15:487. https://doi.org/10.3390/ph15040487
Yang H-L, Bai L-F, Geng Z-R et al (2023) Carbon quantum dots: Preparation, optical properties, and biomedical applications. Mater Today Adv 18:100376. https://doi.org/10.1016/j.mtadv.2023.100376
Yao Y, Zhang T, Tang M (2023) The DNA damage potential of quantum dots: toxicity, mechanism and challenge. Environ Pollut 317:120676. https://doi.org/10.1016/j.envpol.2022.120676
Zhu X, Zhou Y, Yan S et al (2024) Herbal medicine-inspired Carbon Quantum dots with Antibiosis and Hemostasis effects for promoting Wound Healing. ACS Appl Mater Interfaces Acsami. https://doi.org/10.1021/acsami.3c18418. .3c18418
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Khyati Koul: conceptualization, visualization, writing— original draft. Ishwerpreet Kaur Jawanda: conceptualization, visualization, writing— original draftThomson Soni: formal analysis, writing-review and editingPranjali Singh: writing-review and editing Divyani Sharma: writing-review and editing Seema Kumari: conceptualization, Investigation, formal analysis, writing-review and editing.
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Koul, K., Jawanda, I.K., Soni, T. et al. Quantum dots: a next generation approach for pathogenic microbial biofilm inhibition; mechanistic insights, existing challenges, and future potential. Arch Microbiol 206, 158 (2024). https://doi.org/10.1007/s00203-024-03919-3
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DOI: https://doi.org/10.1007/s00203-024-03919-3