Abstract
A strictly anaerobic, motile bacterium, designated as strain Ai-910T, was isolated from the sludge of an anaerobic digestion tank in China. Cells were Gram-stain-negative rods. Optimal growth was observed at 38 °C (growth range 25–42 °C), pH 8.5 (growth range 5.5–10.5), and under a NaCl concentration of 0.06% (w/v) (range 0–2.0%). Major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The respiratory quinone was MK-7. Using xylose as the growth substrate, succinate was produced as the fermentation product. Phylogenetic analysis based on the 16 S rRNA gene sequences indicated that strain Ai-910T formed a distinct phylogenetic lineage that reflects a new genus in the family Marinilabiliaceae, sharing high similarities to Alkaliflexus imshenetskii Z-7010T (92.78%), Alkalitalea saponilacus SC/BZ-SP2T (92.51%), and Geofilum rubicundum JAM-BA0501T (92.36%). Genomic similarity (average nucleotide identity and digital DNA-DNA hybridization) values between strain Ai-910T and its phylogenetic neighbors were below 65.27 and 16.90%, respectively, indicating that strain Ai-910T represented a novel species. The average amino acid identity between strain Ai-910T and other related members of the family Marinilabiliaceae were below 69.41%, supporting that strain Ai-910T was a member of a new genus within the family Marinilabiliaceae. Phylogenetic, genomic, and phenotypic analysis revealed that strain Ai-910T was distinguished from other phylogenetic relatives within the family Marinilabiliaceae. The genome size was 3.10 Mbp, and the DNA G + C content of the isolate was 42.8 mol%. Collectively, differences of the phenotypic and phylogenetic features of strain Ai-910T from its close relatives suggest that strain Ai-910T represented a novel species in a new genus of the family Marinilabiliaceae, for which the name Xiashengella succiniciproducens gen. nov., sp. nov. was proposed. The type strain of Xiashengella succiniciproducens is Ai-910T (= CGMCC 1.17893T = KCTC 25,304T).
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Abbreviations
- NJ:
-
neighbor-joining
- MP:
-
maximum-parsimony
- ML:
-
maximum-likelihood
- TEM:
-
transmission electron microscopy
- GC-MS:
-
gas chromatography- mass spectrometry
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Acknowledgements
We thank Hong-Can Liu for carrying out the fatty acid measurements, Chun-Li Li and Jing-Nan Liang for carrying out scanning electron microscopy and transmission electron microscopy. We particularly thank Prof. Aharon Oren for the constructive comments on the nomenclatures of the strain Ai-910T.
Funding
This work was supported by the National Basic Research Program of China (2019YFC1905000), the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700), the CAS-NSTDA Joint Research Program (NO. 153211KYSB20200039), National Natural Science Foundation of China General Program 32270084, grants of the CAS Engineering Laboratory for Advanced Microbial Technology of Agriculture, Chinese Academy of Sciences (KFJ-PTXM-016).
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CY Jiang, SJ Liu and Y Jiang designed and coordinated the study. HJ Huang, X Zhang, XW Sun, B Chen, and R. Abdugheni identified physiologic and chemotaxonomic characteristics. HJ Huang and N Zhou isolated the strain. HJ Huang, XT Li and Y Deng analyzed the genome. HJ Huang, TJ Zhang and Y Liu collected the samples. HJ Huang wrote the manuscript.
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Huang, HJ., Zhang, X., Sun, XW. et al. Xiashengella succiniciproducens gen. nov., sp. nov., a succinate-producing bacterium isolated from an anaerobic digestion tank in the family Marinilabiliaceae of the order Bacteroidales. Arch Microbiol 206, 141 (2024). https://doi.org/10.1007/s00203-024-03909-5
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DOI: https://doi.org/10.1007/s00203-024-03909-5